Molecular characteristics and spatial distribution of adult human corneal cell subtypes

Abstract Bulk RNA sequencing of a tissue captures the gene expression profile from all cell types combined. Single-cell RNA sequencing identifies discrete cell-signatures based on transcriptomic identities. Six adult human corneas were processed for single-cell RNAseq and 16 cell clusters were bioin...

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Autores principales:	Ann J. Ligocki, Wen Fury, Christian Gutierrez, Christina Adler, Tao Yang, Min Ni, Yu Bai, Yi Wei, Guillermo L. Lehmann, Carmelo Romano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8931ed3b7ba64dc9b7451038923ca334
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spelling	oai:doaj.org-article:8931ed3b7ba64dc9b7451038923ca3342021-12-02T15:07:54ZMolecular characteristics and spatial distribution of adult human corneal cell subtypes10.1038/s41598-021-94933-82045-2322https://doaj.org/article/8931ed3b7ba64dc9b7451038923ca3342021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94933-8https://doaj.org/toc/2045-2322Abstract Bulk RNA sequencing of a tissue captures the gene expression profile from all cell types combined. Single-cell RNA sequencing identifies discrete cell-signatures based on transcriptomic identities. Six adult human corneas were processed for single-cell RNAseq and 16 cell clusters were bioinformatically identified. Based on their transcriptomic signatures and RNAscope results using representative cluster marker genes on human cornea cross-sections, these clusters were confirmed to be stromal keratocytes, endothelium, several subtypes of corneal epithelium, conjunctival epithelium, and supportive cells in the limbal stem cell niche. The complexity of the epithelial cell layer was captured by eight distinct corneal clusters and three conjunctival clusters. These were further characterized by enriched biological pathways and molecular characteristics which revealed novel groupings related to development, function, and location within the epithelial layer. Moreover, epithelial subtypes were found to reflect their initial generation in the limbal region, differentiation, and migration through to mature epithelial cells. The single-cell map of the human cornea deepens the knowledge of the cellular subsets of the cornea on a whole genome transcriptional level. This information can be applied to better understand normal corneal biology, serve as a reference to understand corneal disease pathology, and provide potential insights into therapeutic approaches.Ann J. LigockiWen FuryChristian GutierrezChristina AdlerTao YangMin NiYu BaiYi WeiGuillermo L. LehmannCarmelo RomanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ann J. Ligocki Wen Fury Christian Gutierrez Christina Adler Tao Yang Min Ni Yu Bai Yi Wei Guillermo L. Lehmann Carmelo Romano Molecular characteristics and spatial distribution of adult human corneal cell subtypes
description	Abstract Bulk RNA sequencing of a tissue captures the gene expression profile from all cell types combined. Single-cell RNA sequencing identifies discrete cell-signatures based on transcriptomic identities. Six adult human corneas were processed for single-cell RNAseq and 16 cell clusters were bioinformatically identified. Based on their transcriptomic signatures and RNAscope results using representative cluster marker genes on human cornea cross-sections, these clusters were confirmed to be stromal keratocytes, endothelium, several subtypes of corneal epithelium, conjunctival epithelium, and supportive cells in the limbal stem cell niche. The complexity of the epithelial cell layer was captured by eight distinct corneal clusters and three conjunctival clusters. These were further characterized by enriched biological pathways and molecular characteristics which revealed novel groupings related to development, function, and location within the epithelial layer. Moreover, epithelial subtypes were found to reflect their initial generation in the limbal region, differentiation, and migration through to mature epithelial cells. The single-cell map of the human cornea deepens the knowledge of the cellular subsets of the cornea on a whole genome transcriptional level. This information can be applied to better understand normal corneal biology, serve as a reference to understand corneal disease pathology, and provide potential insights into therapeutic approaches.
format	article
author	Ann J. Ligocki Wen Fury Christian Gutierrez Christina Adler Tao Yang Min Ni Yu Bai Yi Wei Guillermo L. Lehmann Carmelo Romano
author_facet	Ann J. Ligocki Wen Fury Christian Gutierrez Christina Adler Tao Yang Min Ni Yu Bai Yi Wei Guillermo L. Lehmann Carmelo Romano
author_sort	Ann J. Ligocki
title	Molecular characteristics and spatial distribution of adult human corneal cell subtypes
title_short	Molecular characteristics and spatial distribution of adult human corneal cell subtypes
title_full	Molecular characteristics and spatial distribution of adult human corneal cell subtypes
title_fullStr	Molecular characteristics and spatial distribution of adult human corneal cell subtypes
title_full_unstemmed	Molecular characteristics and spatial distribution of adult human corneal cell subtypes
title_sort	molecular characteristics and spatial distribution of adult human corneal cell subtypes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8931ed3b7ba64dc9b7451038923ca334
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Molecular characteristics and spatial distribution of adult human corneal cell subtypes

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