Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.

At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar material (PCM) and a robust increase in microtubule-organizing activity. CEP215 is one of the major PCM components which accumulates at the centrosome during mitosis. The...

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Autores principales:	Seongjae Kim, Kunsoo Rhee
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/893fa46ae3714133914273d4bd2de201
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spelling	oai:doaj.org-article:893fa46ae3714133914273d4bd2de2012021-11-18T08:36:17ZImportance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.1932-620310.1371/journal.pone.0087016https://doaj.org/article/893fa46ae3714133914273d4bd2de2012014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466316/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar material (PCM) and a robust increase in microtubule-organizing activity. CEP215 is one of the major PCM components which accumulates at the centrosome during mitosis. The depletion phenotypes indicate that CEP215 is essential for centrosome maturation and bipolar spindle formation. Here, we performed a series of knockdown-rescue experiments to link the protein-protein interaction properties of CEP215 to its biological functions. The results showed that CEP215 and pericentrin, another major PCM component, is interdependent for their accumulation at the spindle poles during mitosis. As a result, The CEP215-pericentrin interaction is required for centrosome maturation and subsequent bipolar spindle formation during mitosis. On the other hand, CEP215 interaction with γ-tubulin is dispensable for centrosome maturation. Our results provide an insight how PCM components are assembled to form a spindle pole during mitosis.Seongjae KimKunsoo RheePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87016 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seongjae Kim Kunsoo Rhee Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.
description	At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar material (PCM) and a robust increase in microtubule-organizing activity. CEP215 is one of the major PCM components which accumulates at the centrosome during mitosis. The depletion phenotypes indicate that CEP215 is essential for centrosome maturation and bipolar spindle formation. Here, we performed a series of knockdown-rescue experiments to link the protein-protein interaction properties of CEP215 to its biological functions. The results showed that CEP215 and pericentrin, another major PCM component, is interdependent for their accumulation at the spindle poles during mitosis. As a result, The CEP215-pericentrin interaction is required for centrosome maturation and subsequent bipolar spindle formation during mitosis. On the other hand, CEP215 interaction with γ-tubulin is dispensable for centrosome maturation. Our results provide an insight how PCM components are assembled to form a spindle pole during mitosis.
format	article
author	Seongjae Kim Kunsoo Rhee
author_facet	Seongjae Kim Kunsoo Rhee
author_sort	Seongjae Kim
title	Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.
title_short	Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.
title_full	Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.
title_fullStr	Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.
title_full_unstemmed	Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.
title_sort	importance of the cep215-pericentrin interaction for centrosome maturation during mitosis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/893fa46ae3714133914273d4bd2de201
work_keys_str_mv	AT seongjaekim importanceofthecep215pericentrininteractionforcentrosomematurationduringmitosis AT kunsoorhee importanceofthecep215pericentrininteractionforcentrosomematurationduringmitosis
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Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis.

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