Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania
The presence ofTaenia solium DNA from eggs in soils around the households in four Tanzanian villages in Kongwa district were analysed in relation to seasonal fluctuations and infection risk implications. A total of 192 pooled soil samples from five sampling points per household were examined by drop...
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Taylor & Francis Group
2020
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oai:doaj.org-article:8943de92afbd432daaea5f09215290472021-11-04T15:00:43ZSoil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania2000-868610.1080/20008686.2020.1772668https://doaj.org/article/8943de92afbd432daaea5f09215290472020-01-01T00:00:00Zhttp://dx.doi.org/10.1080/20008686.2020.1772668https://doaj.org/toc/2000-8686The presence ofTaenia solium DNA from eggs in soils around the households in four Tanzanian villages in Kongwa district were analysed in relation to seasonal fluctuations and infection risk implications. A total of 192 pooled soil samples from five sampling points per household were examined by droplet digital Polymerase Chain Reaction (ddPCR) from 96 pig-keeping households both during the dry and rainy seasons. The pooled samples were first processed by a flotation-double sieving technique, followed by screening for worm DNA employing universal primers targeting the mitochondrial cytochrome c oxidase subunit I (cox1) gene of human taeniid species and some other helminths. All DNA positive samples were later confirmed by a specific ddPCR probe assay targeting the mitochondrial cox1 gene of T. solium. A total of 17.2% (n = 33) samples were positive with the universal ddPCR, whereas T. solium DNA was confirmed by the specific ddPCR only in 3.1% (n = 3) of the surveyed households. The detection of T. solium DNA in this study spells out a low risk of exposure to T. solium eggs from contaminated household soil. Based on our results, ddPCR seems to be a promising technology for screening T. solium eggs in soil.Justine Daudi MaganiraWinifrida KidimaChacha John MwitaPeter HalvarssonJohan HöglundTaylor & Francis Grouparticletaenia soliumeggssoil contaminationddpcrkongwatanzaniaInfectious and parasitic diseasesRC109-216ENInfection Ecology & Epidemiology, Vol 10, Iss 1 (2020) |
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taenia solium eggs soil contamination ddpcr kongwa tanzania Infectious and parasitic diseases RC109-216 |
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taenia solium eggs soil contamination ddpcr kongwa tanzania Infectious and parasitic diseases RC109-216 Justine Daudi Maganira Winifrida Kidima Chacha John Mwita Peter Halvarsson Johan Höglund Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania |
| description |
The presence ofTaenia solium DNA from eggs in soils around the households in four Tanzanian villages in Kongwa district were analysed in relation to seasonal fluctuations and infection risk implications. A total of 192 pooled soil samples from five sampling points per household were examined by droplet digital Polymerase Chain Reaction (ddPCR) from 96 pig-keeping households both during the dry and rainy seasons. The pooled samples were first processed by a flotation-double sieving technique, followed by screening for worm DNA employing universal primers targeting the mitochondrial cytochrome c oxidase subunit I (cox1) gene of human taeniid species and some other helminths. All DNA positive samples were later confirmed by a specific ddPCR probe assay targeting the mitochondrial cox1 gene of T. solium. A total of 17.2% (n = 33) samples were positive with the universal ddPCR, whereas T. solium DNA was confirmed by the specific ddPCR only in 3.1% (n = 3) of the surveyed households. The detection of T. solium DNA in this study spells out a low risk of exposure to T. solium eggs from contaminated household soil. Based on our results, ddPCR seems to be a promising technology for screening T. solium eggs in soil. |
| format |
article |
| author |
Justine Daudi Maganira Winifrida Kidima Chacha John Mwita Peter Halvarsson Johan Höglund |
| author_facet |
Justine Daudi Maganira Winifrida Kidima Chacha John Mwita Peter Halvarsson Johan Höglund |
| author_sort |
Justine Daudi Maganira |
| title |
Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania |
| title_short |
Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania |
| title_full |
Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania |
| title_fullStr |
Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania |
| title_full_unstemmed |
Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania |
| title_sort |
soil contamination by taenia solium egg dna in rural villages in kongwa district, tanzania |
| publisher |
Taylor & Francis Group |
| publishDate |
2020 |
| url |
https://doaj.org/article/8943de92afbd432daaea5f0921529047 |
| work_keys_str_mv |
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