Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells

Wei Chen,1 Qilin Sun,1 Jing Ju,1 Wenjie Chen,1 Xuelan Zhao,1 Yu Zhang,1 Yehong Yang2 1Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai 200040, China; 2Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai 200040, China Introduction: Oxidative stress plays an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen W, Sun Q, Ju J, Zhao X, Zhang Y, Yang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	SOD2 oxidative stress apoptosis oxidation H9C2 cell siRNA high glucose Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/894c57fa4d4343498c516e1e44a70d63
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:894c57fa4d4343498c516e1e44a70d63
record_format	dspace
spelling	oai:doaj.org-article:894c57fa4d4343498c516e1e44a70d632021-12-02T04:03:14ZAstragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells1178-7007https://doaj.org/article/894c57fa4d4343498c516e1e44a70d632018-10-01T00:00:00Zhttps://www.dovepress.com/astragalus-polysaccharides-inhibit-oxidation-in-high-glucose-challenge-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Wei Chen,1 Qilin Sun,1 Jing Ju,1 Wenjie Chen,1 Xuelan Zhao,1 Yu Zhang,1 Yehong Yang2 1Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai 200040, China; 2Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai 200040, China Introduction: Oxidative stress plays an important role in the development of diabetic cardiomyopathy (DCM). Previously, we reported that Astragalus polysaccharides (APS) improved DCM by inhibition of cardiac oxidative stress. In this study, we evaluated the beneficial effect of APS on high glucose-induced oxidative stress in cardiomyocytes in vitro. Materials and methods: H9C2 cells were cultured in the presence of high concentration of glucose or transfected with siRNASOD2, followed by APS treatment. The cellular mitochondrial ultrastructure was observed using a transmission electron microscope. Cell apoptosis was detected using hairpin oligonucleotide probes and quantified by flow cytometry analysis. Superoxide production was determined by immunohistochemistry using the fluorescent dye dihydroethidium (DHE). Nitrotyrosine and 8-OH-dG antibodies were employed to detect oxidative damage to cytoplasmic proteins and oxidative stress in the nuclei, respectively. Superoxide dismutase (SOD) activity was measured utilizing the SOD Assay Kit, and SOD protein levels were analyzed by Western blotting. Results: APS treatment protected cellular mitochondrial ultrastructure, reduced cell apoptosis (hairpin-1), inhibited cellular superoxide production (DHE), and reduced oxidative damage to cytoplasmic proteins (nitrotyrosine) and oxidative stress in the nuclei (8-OH-dG) in high glucose-induced and/or SOD2-silenced H9C2 cells, together with induction of SOD2 enzyme activity and increase of protein levels. Conclusion: Our findings indicated the beneficial effect of APS on high glucose-challenged H9C2 cells, which was associated with inhibition of oxidative stress in vitro. Keywords: superoxide dismutases, oxidative stress, apoptosis, cardiomyocyte, ROS, diabetic cardiomyopathyChen WSun QJu JChen WZhao XZhang YYang YDove Medical PressarticleSOD2oxidative stressapoptosisoxidationH9C2 cellsiRNAhigh glucoseSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 11, Pp 673-681 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	SOD2 oxidative stress apoptosis oxidation H9C2 cell siRNA high glucose Specialties of internal medicine RC581-951
spellingShingle	SOD2 oxidative stress apoptosis oxidation H9C2 cell siRNA high glucose Specialties of internal medicine RC581-951 Chen W Sun Q Ju J Chen W Zhao X Zhang Y Yang Y Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells
description	Wei Chen,1 Qilin Sun,1 Jing Ju,1 Wenjie Chen,1 Xuelan Zhao,1 Yu Zhang,1 Yehong Yang2 1Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai 200040, China; 2Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai 200040, China Introduction: Oxidative stress plays an important role in the development of diabetic cardiomyopathy (DCM). Previously, we reported that Astragalus polysaccharides (APS) improved DCM by inhibition of cardiac oxidative stress. In this study, we evaluated the beneficial effect of APS on high glucose-induced oxidative stress in cardiomyocytes in vitro. Materials and methods: H9C2 cells were cultured in the presence of high concentration of glucose or transfected with siRNASOD2, followed by APS treatment. The cellular mitochondrial ultrastructure was observed using a transmission electron microscope. Cell apoptosis was detected using hairpin oligonucleotide probes and quantified by flow cytometry analysis. Superoxide production was determined by immunohistochemistry using the fluorescent dye dihydroethidium (DHE). Nitrotyrosine and 8-OH-dG antibodies were employed to detect oxidative damage to cytoplasmic proteins and oxidative stress in the nuclei, respectively. Superoxide dismutase (SOD) activity was measured utilizing the SOD Assay Kit, and SOD protein levels were analyzed by Western blotting. Results: APS treatment protected cellular mitochondrial ultrastructure, reduced cell apoptosis (hairpin-1), inhibited cellular superoxide production (DHE), and reduced oxidative damage to cytoplasmic proteins (nitrotyrosine) and oxidative stress in the nuclei (8-OH-dG) in high glucose-induced and/or SOD2-silenced H9C2 cells, together with induction of SOD2 enzyme activity and increase of protein levels. Conclusion: Our findings indicated the beneficial effect of APS on high glucose-challenged H9C2 cells, which was associated with inhibition of oxidative stress in vitro. Keywords: superoxide dismutases, oxidative stress, apoptosis, cardiomyocyte, ROS, diabetic cardiomyopathy
format	article
author	Chen W Sun Q Ju J Chen W Zhao X Zhang Y Yang Y
author_facet	Chen W Sun Q Ju J Chen W Zhao X Zhang Y Yang Y
author_sort	Chen W
title	Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells
title_short	Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells
title_full	Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells
title_fullStr	Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells
title_full_unstemmed	Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells
title_sort	astragalus polysaccharides inhibit oxidation in high glucose-challenged or sod2-silenced h9c2 cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/894c57fa4d4343498c516e1e44a70d63
work_keys_str_mv	AT chenw astragaluspolysaccharidesinhibitoxidationinhighglucosechallengedorsod2silencedh9c2cells AT sunq astragaluspolysaccharidesinhibitoxidationinhighglucosechallengedorsod2silencedh9c2cells AT juj astragaluspolysaccharidesinhibitoxidationinhighglucosechallengedorsod2silencedh9c2cells AT chenw astragaluspolysaccharidesinhibitoxidationinhighglucosechallengedorsod2silencedh9c2cells AT zhaox astragaluspolysaccharidesinhibitoxidationinhighglucosechallengedorsod2silencedh9c2cells AT zhangy astragaluspolysaccharidesinhibitoxidationinhighglucosechallengedorsod2silencedh9c2cells AT yangy astragaluspolysaccharidesinhibitoxidationinhighglucosechallengedorsod2silencedh9c2cells
_version_	1718401443913793536

Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells

Ejemplares similares