Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.

Heavy-metal-tolerant bacteria, GIMN1.004(T), was isolated from mine soils of Dabaoshan in South China, which were acidic (pH 2-4) and polluted with heavy metals. The isolation was Gram-negative, aerobic, non-spore-forming, and rod-shaped bacteria having a cellular width of 0.5-0.6 µm and a length of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Honghui Zhu, Jianhua Guo, Meibiao Chen, Guangda Feng, Qing Yao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89571326cfcf476982e4cd6379b86c1b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:89571326cfcf476982e4cd6379b86c1b
record_format	dspace
spelling	oai:doaj.org-article:89571326cfcf476982e4cd6379b86c1b2021-11-18T08:06:28ZBurkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.1932-620310.1371/journal.pone.0050225https://doaj.org/article/89571326cfcf476982e4cd6379b86c1b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226514/?tool=EBIhttps://doaj.org/toc/1932-6203Heavy-metal-tolerant bacteria, GIMN1.004(T), was isolated from mine soils of Dabaoshan in South China, which were acidic (pH 2-4) and polluted with heavy metals. The isolation was Gram-negative, aerobic, non-spore-forming, and rod-shaped bacteria having a cellular width of 0.5-0.6 µm and a length of 1.3-1.8 µm. They showed a normal growth pattern at pH 4.0-9.0 in a temperature ranging from 5 °C to 40 °C.The organism contained ubiquinone Q-8 as the predominant isoprenoid quinine, and C(16:0), summed feature 8 (C(18:1)ω7c and C(18:1)ω6c), C(18:0), summed feature 3 (C(16:1)ω7c or iso-C(15:0) 2-OH), C(17:0) cyclo, C(18:1)ω9c, C(19:0) cyclo ω8c, C(14:0) as major fatty acid. These profiles were similar to those reported for Burkholderia species. The DNA G+C % of this strain was 61.6%. Based on the similarity to 16S rRNA gene sequence, GIMN1.004(T) was considered to be in the genus Burkholderia. The similarities of 16S rRNA gene sequence between strain GIMN1.004(T) and members of the genus Burkholderia were 96-99.4%, indicating that this novel strain was phylogenetically related to members of that genus. The novel strain showed the highest sequence similarities to Burkholderia soli DSM 18235(T) (99.4%); Levels of DNA-DNA hybridization with DSM 18235(T) was 25%. Physiological and biochemical tests including cell wall composition analysis, differentiated phenotype of this strain from that closely related Burkholderia species. The isolation had great tolerance to cadmium with MIC of 22 mmol/L, and adsorbability of 144.94 mg/g cadmium,and it was found to exhibit antibiotic resistance characteristics. The adsorptive mechanism of GIMN1.004(T) for cadmium depended on the action of the amide,carboxy and phosphate of cell surface and producing low-molecular-weight (LMW) organic acids to complex or chelated Cd(2+).Therefore, the strain GIMN1.004(T) represented a new cadmium resistance species, which was tentatively named as Burkholderia dabaoshanensis sp. nov. The strain type is GIMN1.004(T) (= CCTCC M 209109(T) = NRRL B-59553(T)).Honghui ZhuJianhua GuoMeibiao ChenGuangda FengQing YaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e50225 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Honghui Zhu Jianhua Guo Meibiao Chen Guangda Feng Qing Yao Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.
description	Heavy-metal-tolerant bacteria, GIMN1.004(T), was isolated from mine soils of Dabaoshan in South China, which were acidic (pH 2-4) and polluted with heavy metals. The isolation was Gram-negative, aerobic, non-spore-forming, and rod-shaped bacteria having a cellular width of 0.5-0.6 µm and a length of 1.3-1.8 µm. They showed a normal growth pattern at pH 4.0-9.0 in a temperature ranging from 5 °C to 40 °C.The organism contained ubiquinone Q-8 as the predominant isoprenoid quinine, and C(16:0), summed feature 8 (C(18:1)ω7c and C(18:1)ω6c), C(18:0), summed feature 3 (C(16:1)ω7c or iso-C(15:0) 2-OH), C(17:0) cyclo, C(18:1)ω9c, C(19:0) cyclo ω8c, C(14:0) as major fatty acid. These profiles were similar to those reported for Burkholderia species. The DNA G+C % of this strain was 61.6%. Based on the similarity to 16S rRNA gene sequence, GIMN1.004(T) was considered to be in the genus Burkholderia. The similarities of 16S rRNA gene sequence between strain GIMN1.004(T) and members of the genus Burkholderia were 96-99.4%, indicating that this novel strain was phylogenetically related to members of that genus. The novel strain showed the highest sequence similarities to Burkholderia soli DSM 18235(T) (99.4%); Levels of DNA-DNA hybridization with DSM 18235(T) was 25%. Physiological and biochemical tests including cell wall composition analysis, differentiated phenotype of this strain from that closely related Burkholderia species. The isolation had great tolerance to cadmium with MIC of 22 mmol/L, and adsorbability of 144.94 mg/g cadmium,and it was found to exhibit antibiotic resistance characteristics. The adsorptive mechanism of GIMN1.004(T) for cadmium depended on the action of the amide,carboxy and phosphate of cell surface and producing low-molecular-weight (LMW) organic acids to complex or chelated Cd(2+).Therefore, the strain GIMN1.004(T) represented a new cadmium resistance species, which was tentatively named as Burkholderia dabaoshanensis sp. nov. The strain type is GIMN1.004(T) (= CCTCC M 209109(T) = NRRL B-59553(T)).
format	article
author	Honghui Zhu Jianhua Guo Meibiao Chen Guangda Feng Qing Yao
author_facet	Honghui Zhu Jianhua Guo Meibiao Chen Guangda Feng Qing Yao
author_sort	Honghui Zhu
title	Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.
title_short	Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.
title_full	Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.
title_fullStr	Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.
title_full_unstemmed	Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.
title_sort	burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from dabaoshan mining area soil in china.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/89571326cfcf476982e4cd6379b86c1b
work_keys_str_mv	AT honghuizhu burkholderiadabaoshanensisspnovaheavymetaltolerantbacteriaisolatedfromdabaoshanminingareasoilinchina AT jianhuaguo burkholderiadabaoshanensisspnovaheavymetaltolerantbacteriaisolatedfromdabaoshanminingareasoilinchina AT meibiaochen burkholderiadabaoshanensisspnovaheavymetaltolerantbacteriaisolatedfromdabaoshanminingareasoilinchina AT guangdafeng burkholderiadabaoshanensisspnovaheavymetaltolerantbacteriaisolatedfromdabaoshanminingareasoilinchina AT qingyao burkholderiadabaoshanensisspnovaheavymetaltolerantbacteriaisolatedfromdabaoshanminingareasoilinchina
_version_	1718422241599815680

Burkholderia dabaoshanensis sp. nov., a heavy-metal-tolerant bacteria isolated from Dabaoshan mining area soil in China.

Ejemplares similares