Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity

Abstract To elucidate a mechanism for enhancing mung bean seedlings’ growth under microgravity conditions, we measured growth, gene expression, and enzyme activity under clinorotation (20 rpm), and compared data obtained to those grown under normal gravity conditions (control). An increase in fresh...

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Autores principales:	Shusaku Nakajima, Masayasu Nagata, Akifumi Ikehata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/895e66d448d24b5d9a6a6cf28ffca178
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spelling	oai:doaj.org-article:895e66d448d24b5d9a6a6cf28ffca1782021-12-02T17:01:14ZMechanism for enhancing the growth of mung bean seedlings under simulated microgravity10.1038/s41526-021-00156-62373-8065https://doaj.org/article/895e66d448d24b5d9a6a6cf28ffca1782021-07-01T00:00:00Zhttps://doi.org/10.1038/s41526-021-00156-6https://doaj.org/toc/2373-8065Abstract To elucidate a mechanism for enhancing mung bean seedlings’ growth under microgravity conditions, we measured growth, gene expression, and enzyme activity under clinorotation (20 rpm), and compared data obtained to those grown under normal gravity conditions (control). An increase in fresh weight, water content, and lengths were observed in the clinostat seedlings, compared to those of the control seedlings. Real-time PCR showed that aquaporin expression and the amylase gene were upregulated under clinorotation. Additionally, seedlings under clinorotation exhibited a significantly higher amylase activity. Near-infrared image showed that there was no restriction of water evaporation from the seedlings under clinorotation. Therefore, these results indicate that simulated microgravity could induce water uptake, resulting in enhanced amylase activity and seedling growth. Upregulated aquaporin expression could be the first trigger for enhanced growth under clinorotation. We speculated that the seedlings under clinorotation do not use energy against gravitational force and consumed surplus energy for enhanced growth.Shusaku NakajimaMasayasu NagataAkifumi IkehataNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 7, Iss 1, Pp 1-5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Shusaku Nakajima Masayasu Nagata Akifumi Ikehata Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
description	Abstract To elucidate a mechanism for enhancing mung bean seedlings’ growth under microgravity conditions, we measured growth, gene expression, and enzyme activity under clinorotation (20 rpm), and compared data obtained to those grown under normal gravity conditions (control). An increase in fresh weight, water content, and lengths were observed in the clinostat seedlings, compared to those of the control seedlings. Real-time PCR showed that aquaporin expression and the amylase gene were upregulated under clinorotation. Additionally, seedlings under clinorotation exhibited a significantly higher amylase activity. Near-infrared image showed that there was no restriction of water evaporation from the seedlings under clinorotation. Therefore, these results indicate that simulated microgravity could induce water uptake, resulting in enhanced amylase activity and seedling growth. Upregulated aquaporin expression could be the first trigger for enhanced growth under clinorotation. We speculated that the seedlings under clinorotation do not use energy against gravitational force and consumed surplus energy for enhanced growth.
format	article
author	Shusaku Nakajima Masayasu Nagata Akifumi Ikehata
author_facet	Shusaku Nakajima Masayasu Nagata Akifumi Ikehata
author_sort	Shusaku Nakajima
title	Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_short	Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_full	Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_fullStr	Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_full_unstemmed	Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
title_sort	mechanism for enhancing the growth of mung bean seedlings under simulated microgravity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/895e66d448d24b5d9a6a6cf28ffca178
work_keys_str_mv	AT shusakunakajima mechanismforenhancingthegrowthofmungbeanseedlingsundersimulatedmicrogravity AT masayasunagata mechanismforenhancingthegrowthofmungbeanseedlingsundersimulatedmicrogravity AT akifumiikehata mechanismforenhancingthegrowthofmungbeanseedlingsundersimulatedmicrogravity
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Mechanism for enhancing the growth of mung bean seedlings under simulated microgravity

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