Diagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)

Aim of this study is to report a laboratory investigation performed following the isolation of <i>Brucella ovis</i>, causing ovine epididymitis, in a traditional sheep farm in Sicily (South Italy). This disease represents a newly emerging risk for Italian livestock and is listed among di...

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Autores principales:	Paola Galluzzo, Sergio Migliore, Silvana Cascio, Santino Barreca, Marilena Alfano, Antonina Tagliarini, Anna Candela, Chiara Piraino, Lucia Galuppo, Lucia Condorelli, Hany A. Hussein, Manuela Tittarelli, Giuseppina Chiarenza
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Brucella ovis</i> ovine epididymitis rams ewes diagnosis Medicine R
Acceso en línea:	https://doaj.org/article/895ec2300f2342228fec34a0a1beefa6
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spelling	oai:doaj.org-article:895ec2300f2342228fec34a0a1beefa62021-11-25T18:38:31ZDiagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)10.3390/pathogens101114722076-0817https://doaj.org/article/895ec2300f2342228fec34a0a1beefa62021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1472https://doaj.org/toc/2076-0817Aim of this study is to report a laboratory investigation performed following the isolation of <i>Brucella ovis</i>, causing ovine epididymitis, in a traditional sheep farm in Sicily (South Italy). This disease represents a newly emerging risk for Italian livestock and is listed among diseases of EU priority (EU Reg 2016/429). Blood samples from 56 rams and 143 ewes were analyzed by both Enzyme-Linked Immunosorbent Assay (ELISA) and Complement Fixation Test (CFT). Genital swabs from all rams and 15 lactating ewes were collected to perform real-time PCR. Eighteen serologically positive rams were slaughtered and postmortem-inspected. Samples of testicle, epididymis, lymph nodes, and urine were also collected in order to perform microbiological, molecular, and histopathological analysis. Twelve slaughtered rams showed anatomo-pathological lesions. Real-time PCR for <i>B. ovis</i> BOV_A0504 gene was positive for 13 testicles and epididymis and 11 urine while <i>B. ovis</i> was isolated from epididymis and testicles of 7 slaughtered rams. This is the first exhaustive laboratory report of a microbiological, molecular, and serological pattern of the disease in sheep in Italy. Despite the impact on health and animal welfare, the epidemiology of <i>B. ovis</i> infection is still unknown, particularly in our country where the disease is considered endemic.Paola GalluzzoSergio MiglioreSilvana CascioSantino BarrecaMarilena AlfanoAntonina TagliariniAnna CandelaChiara PirainoLucia GaluppoLucia CondorelliHany A. HusseinManuela TittarelliGiuseppina ChiarenzaMDPI AGarticle<i>Brucella ovis</i>ovine epididymitisramsewesdiagnosisMedicineRENPathogens, Vol 10, Iss 1472, p 1472 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Brucella ovis</i> ovine epididymitis rams ewes diagnosis Medicine R
spellingShingle	<i>Brucella ovis</i> ovine epididymitis rams ewes diagnosis Medicine R Paola Galluzzo Sergio Migliore Silvana Cascio Santino Barreca Marilena Alfano Antonina Tagliarini Anna Candela Chiara Piraino Lucia Galuppo Lucia Condorelli Hany A. Hussein Manuela Tittarelli Giuseppina Chiarenza Diagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)
description	Aim of this study is to report a laboratory investigation performed following the isolation of <i>Brucella ovis</i>, causing ovine epididymitis, in a traditional sheep farm in Sicily (South Italy). This disease represents a newly emerging risk for Italian livestock and is listed among diseases of EU priority (EU Reg 2016/429). Blood samples from 56 rams and 143 ewes were analyzed by both Enzyme-Linked Immunosorbent Assay (ELISA) and Complement Fixation Test (CFT). Genital swabs from all rams and 15 lactating ewes were collected to perform real-time PCR. Eighteen serologically positive rams were slaughtered and postmortem-inspected. Samples of testicle, epididymis, lymph nodes, and urine were also collected in order to perform microbiological, molecular, and histopathological analysis. Twelve slaughtered rams showed anatomo-pathological lesions. Real-time PCR for <i>B. ovis</i> BOV_A0504 gene was positive for 13 testicles and epididymis and 11 urine while <i>B. ovis</i> was isolated from epididymis and testicles of 7 slaughtered rams. This is the first exhaustive laboratory report of a microbiological, molecular, and serological pattern of the disease in sheep in Italy. Despite the impact on health and animal welfare, the epidemiology of <i>B. ovis</i> infection is still unknown, particularly in our country where the disease is considered endemic.
format	article
author	Paola Galluzzo Sergio Migliore Silvana Cascio Santino Barreca Marilena Alfano Antonina Tagliarini Anna Candela Chiara Piraino Lucia Galuppo Lucia Condorelli Hany A. Hussein Manuela Tittarelli Giuseppina Chiarenza
author_facet	Paola Galluzzo Sergio Migliore Silvana Cascio Santino Barreca Marilena Alfano Antonina Tagliarini Anna Candela Chiara Piraino Lucia Galuppo Lucia Condorelli Hany A. Hussein Manuela Tittarelli Giuseppina Chiarenza
author_sort	Paola Galluzzo
title	Diagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)
title_short	Diagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)
title_full	Diagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)
title_fullStr	Diagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)
title_full_unstemmed	Diagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)
title_sort	diagnostic findings in a confirmed outbreak of <i>brucella ovis</i> infection in a traditional sheep farm in sicily (south-italy)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/895ec2300f2342228fec34a0a1beefa6
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Diagnostic Findings in a Confirmed Outbreak of <i>Brucella ovis</i> Infection in a Traditional Sheep Farm in Sicily (South-Italy)

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