A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels

A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels

Abstract Here we describe a homogeneous bioluminescent immunoassay based on the interaction between Fc-tagged SARS-CoV-2 Spike RBD and human ACE2, and its detection by secondary antibodies labeled with NanoLuc luciferase fragments LgBit and SmBit. The assay utility for the discovery of novel inhibit...

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Autores principales: Juliano Alves, Laurie Engel, Renata de Vasconcelos Cabral, Eduardo L. Rodrigues, Liane de Jesus Ribeiro, Luiza M. Higa, Orlando da Costa Ferreira Júnior, Terezinha Marta P. P. Castiñeiras, Isabela de Carvalho Leitão, Amilcar Tanuri, Said A. Goueli, Hicham Zegzouti
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/896cb46d47394f0b9df32dc086b1c970
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