Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

Abstract Irisin is a new muscular regulatory factor that is generated by the cleavage of its precursor protein fibronectin type III domain-containing protein 5 (FNDC5). Irisin promotes fat consumption due to its stimulatory role in the browning of the adipocytes in mice. Currently, there is no repor...

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Autores principales:	Chunbo Cai, Gaojun Xiao, Lili Qian, Shengwang Jiang, Biao Li, Shanshan Xie, Ting Gao, Xiaorong An, Wentao Cui, Kui Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/897409faf60c44d398b6b6f65707db35
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spelling	oai:doaj.org-article:897409faf60c44d398b6b6f65707db352021-12-02T16:08:24ZGene Location, Expression, and Function of FNDC5 in Meishan Pigs10.1038/s41598-017-08406-y2045-2322https://doaj.org/article/897409faf60c44d398b6b6f65707db352017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08406-yhttps://doaj.org/toc/2045-2322Abstract Irisin is a new muscular regulatory factor that is generated by the cleavage of its precursor protein fibronectin type III domain-containing protein 5 (FNDC5). Irisin promotes fat consumption due to its stimulatory role in the browning of the adipocytes in mice. Currently, there is no report on FNDC5 functions in pigs as model animals. In this study, we investigated the expression patterns and functions of FNDC5 in Meishan pigs. Our results showed that FNDC5 gene in Meishan pigs contains five transcripts, all of which can be translated into functional intact irisin proteins. Porcine FNDC5 is mainly expressed in skeletal muscle, with the expression level being significantly higher during the embryonic and juvenile periods than in the adulthood stage. In vitro study showed that FNDC5 stimulates the proliferation and adipogenic differentiation of primary adipocytes isolated from Meishan pigs, and FNDC5 enhances the expression of browning marker genes during adipogenic differentiation. Our study was the first report on FNDC5 expression patterns and functions in pigs. Data from this study provide valuable information related to the study on FNDC5 functions and future development of novel treatment for obesity.Chunbo CaiGaojun XiaoLili QianShengwang JiangBiao LiShanshan XieTing GaoXiaorong AnWentao CuiKui LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chunbo Cai Gaojun Xiao Lili Qian Shengwang Jiang Biao Li Shanshan Xie Ting Gao Xiaorong An Wentao Cui Kui Li Gene Location, Expression, and Function of FNDC5 in Meishan Pigs
description	Abstract Irisin is a new muscular regulatory factor that is generated by the cleavage of its precursor protein fibronectin type III domain-containing protein 5 (FNDC5). Irisin promotes fat consumption due to its stimulatory role in the browning of the adipocytes in mice. Currently, there is no report on FNDC5 functions in pigs as model animals. In this study, we investigated the expression patterns and functions of FNDC5 in Meishan pigs. Our results showed that FNDC5 gene in Meishan pigs contains five transcripts, all of which can be translated into functional intact irisin proteins. Porcine FNDC5 is mainly expressed in skeletal muscle, with the expression level being significantly higher during the embryonic and juvenile periods than in the adulthood stage. In vitro study showed that FNDC5 stimulates the proliferation and adipogenic differentiation of primary adipocytes isolated from Meishan pigs, and FNDC5 enhances the expression of browning marker genes during adipogenic differentiation. Our study was the first report on FNDC5 expression patterns and functions in pigs. Data from this study provide valuable information related to the study on FNDC5 functions and future development of novel treatment for obesity.
format	article
author	Chunbo Cai Gaojun Xiao Lili Qian Shengwang Jiang Biao Li Shanshan Xie Ting Gao Xiaorong An Wentao Cui Kui Li
author_facet	Chunbo Cai Gaojun Xiao Lili Qian Shengwang Jiang Biao Li Shanshan Xie Ting Gao Xiaorong An Wentao Cui Kui Li
author_sort	Chunbo Cai
title	Gene Location, Expression, and Function of FNDC5 in Meishan Pigs
title_short	Gene Location, Expression, and Function of FNDC5 in Meishan Pigs
title_full	Gene Location, Expression, and Function of FNDC5 in Meishan Pigs
title_fullStr	Gene Location, Expression, and Function of FNDC5 in Meishan Pigs
title_full_unstemmed	Gene Location, Expression, and Function of FNDC5 in Meishan Pigs
title_sort	gene location, expression, and function of fndc5 in meishan pigs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/897409faf60c44d398b6b6f65707db35
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Gene Location, Expression, and Function of FNDC5 in Meishan Pigs

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