Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells

Tetracycline-inducible gene expression systems have been used successfully to study gene function in vivo and in vitro renal epithelial models but the effects of the common inducing agent, doxycycline (DOX), on gene expression are not well appreciated. Here, we evaluated the DOX effects on the trans...

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Autores principales:	Hyun Jun Jung, Richard Coleman, Owen M. Woodward, Paul A. Welling
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	doxycycline RNA-seq transcriptional response cell proliferation mIMCD3 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/8977bd0e848e4b948ee8bed0357c0e64
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spelling	oai:doaj.org-article:8977bd0e848e4b948ee8bed0357c0e642021-11-05T09:56:59ZDoxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells1664-042X10.3389/fphys.2021.771691https://doaj.org/article/8977bd0e848e4b948ee8bed0357c0e642021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.771691/fullhttps://doaj.org/toc/1664-042XTetracycline-inducible gene expression systems have been used successfully to study gene function in vivo and in vitro renal epithelial models but the effects of the common inducing agent, doxycycline (DOX), on gene expression are not well appreciated. Here, we evaluated the DOX effects on the transcriptome of a widely used renal epithelial cell model, mIMCD3 cells, to establish a reference. Cells were grown on permeable filter supports in the absence and presence of DOX (3 or 6 days), and genome-wide transcriptome profiles were assessed using RNA-Seq. We found DOX significantly altered the transcriptome profile, changing the abundance of 1,549 transcripts at 3 days and 2,643 transcripts at 6 days. Within 3 days of treatment, DOX significantly decreased the expression of multiple signaling pathways (ERK, cAMP, and Notch) that are associated with cell proliferation and differentiation. Genes associated with cell cycle progression were subsequently downregulated in cells treated with DOX for 6 days, as were genes involved in cellular immune response processes and several cytokines and chemokines, correlating with a remarkable repression of genes encoding cell proliferation markers. The results provide new insight into responses of renal epithelial cells to DOX and a establish a resource for DOX-mediated gene expression systems.Hyun Jun JungRichard ColemanOwen M. WoodwardPaul A. WellingPaul A. WellingFrontiers Media S.A.articledoxycyclineRNA-seqtranscriptional responsecell proliferationmIMCD3PhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	doxycycline RNA-seq transcriptional response cell proliferation mIMCD3 Physiology QP1-981
spellingShingle	doxycycline RNA-seq transcriptional response cell proliferation mIMCD3 Physiology QP1-981 Hyun Jun Jung Richard Coleman Owen M. Woodward Paul A. Welling Paul A. Welling Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells
description	Tetracycline-inducible gene expression systems have been used successfully to study gene function in vivo and in vitro renal epithelial models but the effects of the common inducing agent, doxycycline (DOX), on gene expression are not well appreciated. Here, we evaluated the DOX effects on the transcriptome of a widely used renal epithelial cell model, mIMCD3 cells, to establish a reference. Cells were grown on permeable filter supports in the absence and presence of DOX (3 or 6 days), and genome-wide transcriptome profiles were assessed using RNA-Seq. We found DOX significantly altered the transcriptome profile, changing the abundance of 1,549 transcripts at 3 days and 2,643 transcripts at 6 days. Within 3 days of treatment, DOX significantly decreased the expression of multiple signaling pathways (ERK, cAMP, and Notch) that are associated with cell proliferation and differentiation. Genes associated with cell cycle progression were subsequently downregulated in cells treated with DOX for 6 days, as were genes involved in cellular immune response processes and several cytokines and chemokines, correlating with a remarkable repression of genes encoding cell proliferation markers. The results provide new insight into responses of renal epithelial cells to DOX and a establish a resource for DOX-mediated gene expression systems.
format	article
author	Hyun Jun Jung Richard Coleman Owen M. Woodward Paul A. Welling Paul A. Welling
author_facet	Hyun Jun Jung Richard Coleman Owen M. Woodward Paul A. Welling Paul A. Welling
author_sort	Hyun Jun Jung
title	Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells
title_short	Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells
title_full	Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells
title_fullStr	Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells
title_full_unstemmed	Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells
title_sort	doxycycline changes the transcriptome profile of mimcd3 renal epithelial cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8977bd0e848e4b948ee8bed0357c0e64
work_keys_str_mv	AT hyunjunjung doxycyclinechangesthetranscriptomeprofileofmimcd3renalepithelialcells AT richardcoleman doxycyclinechangesthetranscriptomeprofileofmimcd3renalepithelialcells AT owenmwoodward doxycyclinechangesthetranscriptomeprofileofmimcd3renalepithelialcells AT paulawelling doxycyclinechangesthetranscriptomeprofileofmimcd3renalepithelialcells AT paulawelling doxycyclinechangesthetranscriptomeprofileofmimcd3renalepithelialcells
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Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells

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