Investigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation

Biao-Qi Chen,1 Ranjith Kumar Kankala,1,2 Ai-Zheng Chen,1,2 Ding-Zhu Yang,1 Xiao-Xia Cheng,1 Ni-Na Jiang,1,2 Kai Zhu,3,4 Shi-Bin Wang1,2 1Institute of Biomaterials and Tissue Engineering, 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian, 3Department of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen BQ, Kankala RK, Chen AZ, Yang DZ, Cheng XX, Jiang NN, Zhu K, Wang SB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	poly(L-lactic acid) topography silk fibroin cellular activity osteogenic differentiation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/897847ad7ef24f6789677587ae131978
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:897847ad7ef24f6789677587ae131978
record_format	dspace
spelling	oai:doaj.org-article:897847ad7ef24f6789677587ae1319782021-12-02T00:20:48ZInvestigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation1178-2013https://doaj.org/article/897847ad7ef24f6789677587ae1319782017-03-01T00:00:00Zhttps://www.dovepress.com/investigation-of-silk-fibroin-nanoparticle-decorated-polyl-lactic-acid-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Biao-Qi Chen,1 Ranjith Kumar Kankala,1,2 Ai-Zheng Chen,1,2 Ding-Zhu Yang,1 Xiao-Xia Cheng,1 Ni-Na Jiang,1,2 Kai Zhu,3,4 Shi-Bin Wang1,2 1Institute of Biomaterials and Tissue Engineering, 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian, 3Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, 4Shanghai Institute of Cardiovascular Disease, Shanghai, People’s Republic of China Abstract: Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly(L-lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO2). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment. The in vitro cytotoxicity test of SF/PLLA scaffolds in L929 mouse fibroblast cells indicated good biocompatibility. Then, the in vitro interplay between mouse preosteoblast cell (MC3T3-E1) and various topological structures and biochemical cues were evaluated. The cell adhesion, proliferation, osteogenic differentiation and their relationship with the structures as well as SF content were explored. The SF/PLLA weight ratio (2:8) significantly affected the MC3T3-E1 cells by improving the expression of key players in the regulation of bone formation, ie, alkaline phosphatase (ALP), osteocalcin (OC) and collagen 1 (COL-1). These results suggest not only the importance of surface topography and biochemical cues but also the potential of applying SF/PLLA composite scaffolds as biomaterials in bone tissue engineering. Keywords: super critical fluids, surface topography, bone engineering, cellular adhesion, alkaline phosphataseChen BQKankala RKChen AZYang DZCheng XXJiang NNZhu KWang SBDove Medical Pressarticlepoly(L-lactic acid)topographysilk fibroincellular activityosteogenic differentiationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1877-1890 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	poly(L-lactic acid) topography silk fibroin cellular activity osteogenic differentiation Medicine (General) R5-920
spellingShingle	poly(L-lactic acid) topography silk fibroin cellular activity osteogenic differentiation Medicine (General) R5-920 Chen BQ Kankala RK Chen AZ Yang DZ Cheng XX Jiang NN Zhu K Wang SB Investigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation
description	Biao-Qi Chen,1 Ranjith Kumar Kankala,1,2 Ai-Zheng Chen,1,2 Ding-Zhu Yang,1 Xiao-Xia Cheng,1 Ni-Na Jiang,1,2 Kai Zhu,3,4 Shi-Bin Wang1,2 1Institute of Biomaterials and Tissue Engineering, 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian, 3Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, 4Shanghai Institute of Cardiovascular Disease, Shanghai, People’s Republic of China Abstract: Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly(L-lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO2). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment. The in vitro cytotoxicity test of SF/PLLA scaffolds in L929 mouse fibroblast cells indicated good biocompatibility. Then, the in vitro interplay between mouse preosteoblast cell (MC3T3-E1) and various topological structures and biochemical cues were evaluated. The cell adhesion, proliferation, osteogenic differentiation and their relationship with the structures as well as SF content were explored. The SF/PLLA weight ratio (2:8) significantly affected the MC3T3-E1 cells by improving the expression of key players in the regulation of bone formation, ie, alkaline phosphatase (ALP), osteocalcin (OC) and collagen 1 (COL-1). These results suggest not only the importance of surface topography and biochemical cues but also the potential of applying SF/PLLA composite scaffolds as biomaterials in bone tissue engineering. Keywords: super critical fluids, surface topography, bone engineering, cellular adhesion, alkaline phosphatase
format	article
author	Chen BQ Kankala RK Chen AZ Yang DZ Cheng XX Jiang NN Zhu K Wang SB
author_facet	Chen BQ Kankala RK Chen AZ Yang DZ Cheng XX Jiang NN Zhu K Wang SB
author_sort	Chen BQ
title	Investigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation
title_short	Investigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation
title_full	Investigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation
title_fullStr	Investigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation
title_full_unstemmed	Investigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation
title_sort	investigation of silk fibroin nanoparticle-decorated poly(l-lactic acid) composite scaffolds for osteoblast growth and differentiation
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/897847ad7ef24f6789677587ae131978
work_keys_str_mv	AT chenbq investigationofsilkfibroinnanoparticledecoratedpolyllacticacidcompositescaffoldsforosteoblastgrowthanddifferentiation AT kankalark investigationofsilkfibroinnanoparticledecoratedpolyllacticacidcompositescaffoldsforosteoblastgrowthanddifferentiation AT chenaz investigationofsilkfibroinnanoparticledecoratedpolyllacticacidcompositescaffoldsforosteoblastgrowthanddifferentiation AT yangdz investigationofsilkfibroinnanoparticledecoratedpolyllacticacidcompositescaffoldsforosteoblastgrowthanddifferentiation AT chengxx investigationofsilkfibroinnanoparticledecoratedpolyllacticacidcompositescaffoldsforosteoblastgrowthanddifferentiation AT jiangnn investigationofsilkfibroinnanoparticledecoratedpolyllacticacidcompositescaffoldsforosteoblastgrowthanddifferentiation AT zhuk investigationofsilkfibroinnanoparticledecoratedpolyllacticacidcompositescaffoldsforosteoblastgrowthanddifferentiation AT wangsb investigationofsilkfibroinnanoparticledecoratedpolyllacticacidcompositescaffoldsforosteoblastgrowthanddifferentiation
_version_	1718403813472206848

Investigation of silk fibroin nanoparticle-decorated poly(L-lactic acid) composite scaffolds for osteoblast growth and differentiation

Ejemplares similares