A new perspective on Listeria monocytogenes evolution.

Listeria monocytogenes is a model organism for cellular microbiology and host-pathogen interaction studies and an important food-borne pathogen widespread in the environment, thus representing an attractive model to study the evolution of virulence. The phylogenetic structure of L. monocytogenes was...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marie Ragon, Thierry Wirth, Florian Hollandt, Rachel Lavenir, Marc Lecuit, Alban Le Monnier, Sylvain Brisse
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/897b93a23715409a87644fc87f69c995
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:897b93a23715409a87644fc87f69c995
record_format	dspace
spelling	oai:doaj.org-article:897b93a23715409a87644fc87f69c9952021-11-25T05:47:29ZA new perspective on Listeria monocytogenes evolution.1553-73661553-737410.1371/journal.ppat.1000146https://doaj.org/article/897b93a23715409a87644fc87f69c9952008-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18773117/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Listeria monocytogenes is a model organism for cellular microbiology and host-pathogen interaction studies and an important food-borne pathogen widespread in the environment, thus representing an attractive model to study the evolution of virulence. The phylogenetic structure of L. monocytogenes was determined by sequencing internal portions of seven housekeeping genes (3,288 nucleotides) in 360 representative isolates. Fifty-eight of the 126 disclosed sequence types were grouped into seven well-demarcated clonal complexes (clones) that comprised almost 75% of clinical isolates. Each clone had a unique or dominant serotype (4b for clones 1, 2 and 4, 1/2b for clones 3 and 5, 1/2a for clone 7, and 1/2c for clone 9), with no association of clones with clinical forms of human listeriosis. Homologous recombination was extremely limited (r/m<1 for nucleotides), implying long-term genetic stability of multilocus genotypes over time. Bayesian analysis based on 438 SNPs recovered the three previously defined lineages, plus one unclassified isolate of mixed ancestry. The phylogenetic distribution of serotypes indicated that serotype 4b evolved once from 1/2b, the likely ancestral serotype of lineage I. Serotype 1/2c derived once from 1/2a, with reference strain EGDe (1/2a) likely representing an intermediate evolutionary state. In contrast to housekeeping genes, the virulence factor internalin (InlA) evolved by localized recombination resulting in a mosaic pattern, with convergent evolution indicative of natural selection towards a truncation of InlA protein. This work provides a reference evolutionary framework for future studies on L. monocytogenes epidemiology, ecology, and virulence.Marie RagonThierry WirthFlorian HollandtRachel LavenirMarc LecuitAlban Le MonnierSylvain BrissePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 9, p e1000146 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Marie Ragon Thierry Wirth Florian Hollandt Rachel Lavenir Marc Lecuit Alban Le Monnier Sylvain Brisse A new perspective on Listeria monocytogenes evolution.
description	Listeria monocytogenes is a model organism for cellular microbiology and host-pathogen interaction studies and an important food-borne pathogen widespread in the environment, thus representing an attractive model to study the evolution of virulence. The phylogenetic structure of L. monocytogenes was determined by sequencing internal portions of seven housekeeping genes (3,288 nucleotides) in 360 representative isolates. Fifty-eight of the 126 disclosed sequence types were grouped into seven well-demarcated clonal complexes (clones) that comprised almost 75% of clinical isolates. Each clone had a unique or dominant serotype (4b for clones 1, 2 and 4, 1/2b for clones 3 and 5, 1/2a for clone 7, and 1/2c for clone 9), with no association of clones with clinical forms of human listeriosis. Homologous recombination was extremely limited (r/m<1 for nucleotides), implying long-term genetic stability of multilocus genotypes over time. Bayesian analysis based on 438 SNPs recovered the three previously defined lineages, plus one unclassified isolate of mixed ancestry. The phylogenetic distribution of serotypes indicated that serotype 4b evolved once from 1/2b, the likely ancestral serotype of lineage I. Serotype 1/2c derived once from 1/2a, with reference strain EGDe (1/2a) likely representing an intermediate evolutionary state. In contrast to housekeeping genes, the virulence factor internalin (InlA) evolved by localized recombination resulting in a mosaic pattern, with convergent evolution indicative of natural selection towards a truncation of InlA protein. This work provides a reference evolutionary framework for future studies on L. monocytogenes epidemiology, ecology, and virulence.
format	article
author	Marie Ragon Thierry Wirth Florian Hollandt Rachel Lavenir Marc Lecuit Alban Le Monnier Sylvain Brisse
author_facet	Marie Ragon Thierry Wirth Florian Hollandt Rachel Lavenir Marc Lecuit Alban Le Monnier Sylvain Brisse
author_sort	Marie Ragon
title	A new perspective on Listeria monocytogenes evolution.
title_short	A new perspective on Listeria monocytogenes evolution.
title_full	A new perspective on Listeria monocytogenes evolution.
title_fullStr	A new perspective on Listeria monocytogenes evolution.
title_full_unstemmed	A new perspective on Listeria monocytogenes evolution.
title_sort	new perspective on listeria monocytogenes evolution.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/897b93a23715409a87644fc87f69c995
work_keys_str_mv	AT marieragon anewperspectiveonlisteriamonocytogenesevolution AT thierrywirth anewperspectiveonlisteriamonocytogenesevolution AT florianhollandt anewperspectiveonlisteriamonocytogenesevolution AT rachellavenir anewperspectiveonlisteriamonocytogenesevolution AT marclecuit anewperspectiveonlisteriamonocytogenesevolution AT albanlemonnier anewperspectiveonlisteriamonocytogenesevolution AT sylvainbrisse anewperspectiveonlisteriamonocytogenesevolution AT marieragon newperspectiveonlisteriamonocytogenesevolution AT thierrywirth newperspectiveonlisteriamonocytogenesevolution AT florianhollandt newperspectiveonlisteriamonocytogenesevolution AT rachellavenir newperspectiveonlisteriamonocytogenesevolution AT marclecuit newperspectiveonlisteriamonocytogenesevolution AT albanlemonnier newperspectiveonlisteriamonocytogenesevolution AT sylvainbrisse newperspectiveonlisteriamonocytogenesevolution
_version_	1718414468666359808

A new perspective on Listeria monocytogenes evolution.

Ejemplares similares