Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis

The circ_UBR4 (hsa_circ_0010283) is a novel abnormally overexpressed circRNA in oxidized low-density lipoprotein (ox-LDL)-induced model of atherosclerosis (AS) in human vascular smooth muscle cells (VSMCs). However, its role in the dysfunction of VSMCs remains to be further investigated. Here, we at...

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Autores principales:	Zhang Ying, Zhang Cheng, Chen Zongwei, Wang Meilan
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	circ_ubr4 mir-107 rock1 atherosclerosis vsmcs Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8985149e3f8e432abf261b4f0acfe9c1
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spelling	oai:doaj.org-article:8985149e3f8e432abf261b4f0acfe9c12021-12-05T14:10:41ZBlocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis2391-541210.1515/biol-2021-0044https://doaj.org/article/8985149e3f8e432abf261b4f0acfe9c12021-04-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0044https://doaj.org/toc/2391-5412The circ_UBR4 (hsa_circ_0010283) is a novel abnormally overexpressed circRNA in oxidized low-density lipoprotein (ox-LDL)-induced model of atherosclerosis (AS) in human vascular smooth muscle cells (VSMCs). However, its role in the dysfunction of VSMCs remains to be further investigated. Here, we attempted to explore its role in ox-LDL-induced excessive proliferation and migration in VSMCs by regulating Rho/Rho-associated coiled-coil containing kinase 1 (ROCK1), a therapeutic target of AS. Expression of circ_UBR4 and ROCK1 was upregulated, whereas miR-107 was downregulated in human AS serum and ox-LDL-induced VSMCs. Depletion of circ_UBR4 arrested cell cycle, suppressed cell viability, colony-forming ability, and migration ability, and depressed expression of proliferating cell nuclear antigen and matrix metalloproteinase 2 in VSMCs in spite of the opposite effects of ox-LDL. Notably, ROCK1 upregulation mediated by plasmid transfection or miR-107 deletion could counteract the suppressive role of circ_UBR4 knockdown in ox-LDL-induced VSMCs proliferation, migration, and cell cycle progression. In mechanism, miR-107 was identified as a target of circ_UBR4 to mediate the regulatory effect of circ_UBR4 on ROCK1. circ_UBR4 might be a contributor in human AS partially by regulating VSMCs’ cell proliferation, migration, and cell cycle progression via circ_UBR4/miR-107/ROCK1 pathway.Zhang YingZhang ChengChen ZongweiWang MeilanDe Gruyterarticlecirc_ubr4mir-107rock1atherosclerosisvsmcsBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 419-430 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circ_ubr4 mir-107 rock1 atherosclerosis vsmcs Biology (General) QH301-705.5
spellingShingle	circ_ubr4 mir-107 rock1 atherosclerosis vsmcs Biology (General) QH301-705.5 Zhang Ying Zhang Cheng Chen Zongwei Wang Meilan Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
description	The circ_UBR4 (hsa_circ_0010283) is a novel abnormally overexpressed circRNA in oxidized low-density lipoprotein (ox-LDL)-induced model of atherosclerosis (AS) in human vascular smooth muscle cells (VSMCs). However, its role in the dysfunction of VSMCs remains to be further investigated. Here, we attempted to explore its role in ox-LDL-induced excessive proliferation and migration in VSMCs by regulating Rho/Rho-associated coiled-coil containing kinase 1 (ROCK1), a therapeutic target of AS. Expression of circ_UBR4 and ROCK1 was upregulated, whereas miR-107 was downregulated in human AS serum and ox-LDL-induced VSMCs. Depletion of circ_UBR4 arrested cell cycle, suppressed cell viability, colony-forming ability, and migration ability, and depressed expression of proliferating cell nuclear antigen and matrix metalloproteinase 2 in VSMCs in spite of the opposite effects of ox-LDL. Notably, ROCK1 upregulation mediated by plasmid transfection or miR-107 deletion could counteract the suppressive role of circ_UBR4 knockdown in ox-LDL-induced VSMCs proliferation, migration, and cell cycle progression. In mechanism, miR-107 was identified as a target of circ_UBR4 to mediate the regulatory effect of circ_UBR4 on ROCK1. circ_UBR4 might be a contributor in human AS partially by regulating VSMCs’ cell proliferation, migration, and cell cycle progression via circ_UBR4/miR-107/ROCK1 pathway.
format	article
author	Zhang Ying Zhang Cheng Chen Zongwei Wang Meilan
author_facet	Zhang Ying Zhang Cheng Chen Zongwei Wang Meilan
author_sort	Zhang Ying
title	Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
title_short	Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
title_full	Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
title_fullStr	Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
title_full_unstemmed	Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
title_sort	blocking circ_ubr4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/8985149e3f8e432abf261b4f0acfe9c1
work_keys_str_mv	AT zhangying blockingcircubr4suppressedproliferationmigrationandcellcycleprogressionofhumanvascularsmoothmusclecellsinatherosclerosis AT zhangcheng blockingcircubr4suppressedproliferationmigrationandcellcycleprogressionofhumanvascularsmoothmusclecellsinatherosclerosis AT chenzongwei blockingcircubr4suppressedproliferationmigrationandcellcycleprogressionofhumanvascularsmoothmusclecellsinatherosclerosis AT wangmeilan blockingcircubr4suppressedproliferationmigrationandcellcycleprogressionofhumanvascularsmoothmusclecellsinatherosclerosis
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Blocking circ_UBR4 suppressed proliferation, migration, and cell cycle progression of human vascular smooth muscle cells in atherosclerosis

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