Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma

Simultaneous quantification of multiple psychiatric drugs is important for the therapeutic drug monitoring of psychiatric patients. In addition, it would be highly advantageous if the method could be simple, straightforward, and not time-consuming. A 200 µl plasma sample was deproteinized, drugs wer...

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Autores principales:	Jeremić Aleksandra, Milosavljević Filip, Vladimirov Sandra, Batinić Bojan, Marković Bojan, Jukić Marin
Formato:	article
Lenguaje:	SR
Publicado:	Pharmaceutical Association of Serbia, Belgrade, Serbia 2021
Materias:	sertralin escitalopram risperidon paliperidon hplc-ms/ms Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/8997aeaab98343e5a4bb0f34940beb94
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spelling	oai:doaj.org-article:8997aeaab98343e5a4bb0f34940beb942021-12-05T18:01:41ZValidation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma0004-19632217-876710.5937/arhfarm71-31163https://doaj.org/article/8997aeaab98343e5a4bb0f34940beb942021-01-01T00:00:00Zhttps://scindeks-clanci.ceon.rs/data/pdf/0004-1963/2021/0004-19632105365J.pdfhttps://doaj.org/toc/0004-1963https://doaj.org/toc/2217-8767Simultaneous quantification of multiple psychiatric drugs is important for the therapeutic drug monitoring of psychiatric patients. In addition, it would be highly advantageous if the method could be simple, straightforward, and not time-consuming. A 200 µl plasma sample was deproteinized, drugs were separated by a ZORBAX Eclipse XDB-Phenyl column with the mobile phase composed of acetonitrile and 0.1 % formic acid in water (60:40, v/v), and recorded in the MRM mode by using a positive electrospray source with tandem mass spectrometry detection. The dynamic range was 2-256 ng/ml for all the analyzed drugs, except escitalopram (8-256 ng/ml). Quality control samples were prepared in quintuplicates in three relevant concentrations for each drug. Coefficients of determination (R2 ) were higher than 0.99, while the relative difference between nominal and measured concentrations (RE) and CV were lower than 15% for all targets. High performance liquid chromatography coupled with the mass detector (HPLC-MS/MS) method for simultaneous determination of sertraline, escitalopram, risperidone and paliperidone in human plasma was validated with respect to selectivity, linearity, accuracy, precision, matrix effect and stability. This method has significant advantages in terms of low sample volume (200 µl), short preparation time (3 hours) and short runtime per sample (4 minutes).Jeremić AleksandraMilosavljević FilipVladimirov SandraBatinić BojanMarković BojanJukić MarinPharmaceutical Association of Serbia, Belgrade, Serbiaarticlesertralinescitalopramrisperidonpaliperidonhplc-ms/msPharmacy and materia medicaRS1-441SRArhiv za farmaciju, Vol 71, Iss 5, Pp 365-377 (2021)
institution	DOAJ
collection	DOAJ
language	SR
topic	sertralin escitalopram risperidon paliperidon hplc-ms/ms Pharmacy and materia medica RS1-441
spellingShingle	sertralin escitalopram risperidon paliperidon hplc-ms/ms Pharmacy and materia medica RS1-441 Jeremić Aleksandra Milosavljević Filip Vladimirov Sandra Batinić Bojan Marković Bojan Jukić Marin Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma
description	Simultaneous quantification of multiple psychiatric drugs is important for the therapeutic drug monitoring of psychiatric patients. In addition, it would be highly advantageous if the method could be simple, straightforward, and not time-consuming. A 200 µl plasma sample was deproteinized, drugs were separated by a ZORBAX Eclipse XDB-Phenyl column with the mobile phase composed of acetonitrile and 0.1 % formic acid in water (60:40, v/v), and recorded in the MRM mode by using a positive electrospray source with tandem mass spectrometry detection. The dynamic range was 2-256 ng/ml for all the analyzed drugs, except escitalopram (8-256 ng/ml). Quality control samples were prepared in quintuplicates in three relevant concentrations for each drug. Coefficients of determination (R2 ) were higher than 0.99, while the relative difference between nominal and measured concentrations (RE) and CV were lower than 15% for all targets. High performance liquid chromatography coupled with the mass detector (HPLC-MS/MS) method for simultaneous determination of sertraline, escitalopram, risperidone and paliperidone in human plasma was validated with respect to selectivity, linearity, accuracy, precision, matrix effect and stability. This method has significant advantages in terms of low sample volume (200 µl), short preparation time (3 hours) and short runtime per sample (4 minutes).
format	article
author	Jeremić Aleksandra Milosavljević Filip Vladimirov Sandra Batinić Bojan Marković Bojan Jukić Marin
author_facet	Jeremić Aleksandra Milosavljević Filip Vladimirov Sandra Batinić Bojan Marković Bojan Jukić Marin
author_sort	Jeremić Aleksandra
title	Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma
title_short	Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma
title_full	Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma
title_fullStr	Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma
title_full_unstemmed	Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma
title_sort	validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma
publisher	Pharmaceutical Association of Serbia, Belgrade, Serbia
publishDate	2021
url	https://doaj.org/article/8997aeaab98343e5a4bb0f34940beb94
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Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma

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