Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.

Trafficking of lung dendritic cells (DCs) to the draining lymph node (dLN) is a crucial step for the initiation of T cell responses upon pathogen challenge. However, little is known about the factors that regulate lung DC migration to the dLN. In this study, using a model of influenza infection, we...

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Autores principales:	Matheswaran Kandasamy, Poon C Ying, Adrian W S Ho, Hermi R Sumatoh, Andreas Schlitzer, Timothy R Hughes, David M Kemeny, B Paul Morgan, Florent Ginhoux, Baalasubramanian Sivasankar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8999718c41124fd0a4b58773d930607c
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spelling	oai:doaj.org-article:8999718c41124fd0a4b58773d930607c2021-11-18T06:06:10ZComplement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.1553-73661553-737410.1371/journal.ppat.1003115https://doaj.org/article/8999718c41124fd0a4b58773d930607c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23326231/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Trafficking of lung dendritic cells (DCs) to the draining lymph node (dLN) is a crucial step for the initiation of T cell responses upon pathogen challenge. However, little is known about the factors that regulate lung DC migration to the dLN. In this study, using a model of influenza infection, we demonstrate that complement component C3 is critically required for efficient emigration of DCs from the lung to the dLN. C3 deficiency affect lung DC-mediated viral antigen transport to the dLN, resulting in severely compromised priming of virus-specific T cell responses. Consequently, C3-deficient mice lack effector T cell response in the lungs that affected viral clearance and survival. We further show that direct signaling by C3a and C5a through C3aR and C5aR respectively expressed on lung DCs is required for their efficient trafficking. However, among lung DCs, only CD103(+) DCs make a significant contribution to lung C5a levels and exclusively produce high levels of C3 and C5 during influenza infection. Collectively, our findings show that complement has a profound impact on immune regulation by controlling tissue DC trafficking and highlights a potential utility for complement as an adjuvant in novel vaccine strategies.Matheswaran KandasamyPoon C YingAdrian W S HoHermi R SumatohAndreas SchlitzerTimothy R HughesDavid M KemenyB Paul MorganFlorent GinhouxBaalasubramanian SivasankarPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 1, p e1003115 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Matheswaran Kandasamy Poon C Ying Adrian W S Ho Hermi R Sumatoh Andreas Schlitzer Timothy R Hughes David M Kemeny B Paul Morgan Florent Ginhoux Baalasubramanian Sivasankar Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.
description	Trafficking of lung dendritic cells (DCs) to the draining lymph node (dLN) is a crucial step for the initiation of T cell responses upon pathogen challenge. However, little is known about the factors that regulate lung DC migration to the dLN. In this study, using a model of influenza infection, we demonstrate that complement component C3 is critically required for efficient emigration of DCs from the lung to the dLN. C3 deficiency affect lung DC-mediated viral antigen transport to the dLN, resulting in severely compromised priming of virus-specific T cell responses. Consequently, C3-deficient mice lack effector T cell response in the lungs that affected viral clearance and survival. We further show that direct signaling by C3a and C5a through C3aR and C5aR respectively expressed on lung DCs is required for their efficient trafficking. However, among lung DCs, only CD103(+) DCs make a significant contribution to lung C5a levels and exclusively produce high levels of C3 and C5 during influenza infection. Collectively, our findings show that complement has a profound impact on immune regulation by controlling tissue DC trafficking and highlights a potential utility for complement as an adjuvant in novel vaccine strategies.
format	article
author	Matheswaran Kandasamy Poon C Ying Adrian W S Ho Hermi R Sumatoh Andreas Schlitzer Timothy R Hughes David M Kemeny B Paul Morgan Florent Ginhoux Baalasubramanian Sivasankar
author_facet	Matheswaran Kandasamy Poon C Ying Adrian W S Ho Hermi R Sumatoh Andreas Schlitzer Timothy R Hughes David M Kemeny B Paul Morgan Florent Ginhoux Baalasubramanian Sivasankar
author_sort	Matheswaran Kandasamy
title	Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.
title_short	Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.
title_full	Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.
title_fullStr	Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.
title_full_unstemmed	Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.
title_sort	complement mediated signaling on pulmonary cd103(+) dendritic cells is critical for their migratory function in response to influenza infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8999718c41124fd0a4b58773d930607c
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Complement mediated signaling on pulmonary CD103(+) dendritic cells is critical for their migratory function in response to influenza infection.

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