Probing amphotericin B single channel activity by membrane dipole modifiers.

The effects of dipole modifiers and their structural analogs on the single channel activity of amphotericin B in sterol-containing planar phosphocholine membranes are studied. It is shown that the addition of phloretin in solutions bathing membranes containing cholesterol or ergosterol decreases the...

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Autores principales:	Olga S Ostroumova, Svetlana S Efimova, Ludmila V Schagina
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89a480a8db7442dd8b359875b061c41b
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spelling	oai:doaj.org-article:89a480a8db7442dd8b359875b061c41b2021-11-18T07:29:49ZProbing amphotericin B single channel activity by membrane dipole modifiers.1932-620310.1371/journal.pone.0030261https://doaj.org/article/89a480a8db7442dd8b359875b061c41b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22276169/?tool=EBIhttps://doaj.org/toc/1932-6203The effects of dipole modifiers and their structural analogs on the single channel activity of amphotericin B in sterol-containing planar phosphocholine membranes are studied. It is shown that the addition of phloretin in solutions bathing membranes containing cholesterol or ergosterol decreases the conductance of single amphotericin B channels. Quercetin decreases the channel conductance in cholesterol-containing bilayers while it does not affect the channel conductance in ergosterol-containing membranes. It is demonstrated that the insertion of styryl dyes, such as RH 421, RH 237 or RH 160, in bilayers with either cholesterol or ergosterol leads to the increase of the current amplitude of amphotericin B pores. Introduction of 5α-androstan-3β-ol into a membrane-forming solution increases the amphotericin B channel conductance in a concentration-dependent manner. All the effects are likely to be attributed to the influence of the membrane dipole potential on the conductance of single amphotericin B channels. However, specific interactions of some dipole modifiers with polyene-sterol complexes might also contribute to the activity of single amphotericin B pores. It has been shown that the channel dwell time increases with increasing sterol concentration, and it is higher for cholesterol-containing membranes than for bilayers including ergosterol, 6-ketocholestanol, 7-ketocholestanol or 5α-androstan-3β-ol. These findings suggest that the processes of association/dissociation of channel forming molecules depend on the membrane fluidity.Olga S OstroumovaSvetlana S EfimovaLudmila V SchaginaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30261 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olga S Ostroumova Svetlana S Efimova Ludmila V Schagina Probing amphotericin B single channel activity by membrane dipole modifiers.
description	The effects of dipole modifiers and their structural analogs on the single channel activity of amphotericin B in sterol-containing planar phosphocholine membranes are studied. It is shown that the addition of phloretin in solutions bathing membranes containing cholesterol or ergosterol decreases the conductance of single amphotericin B channels. Quercetin decreases the channel conductance in cholesterol-containing bilayers while it does not affect the channel conductance in ergosterol-containing membranes. It is demonstrated that the insertion of styryl dyes, such as RH 421, RH 237 or RH 160, in bilayers with either cholesterol or ergosterol leads to the increase of the current amplitude of amphotericin B pores. Introduction of 5α-androstan-3β-ol into a membrane-forming solution increases the amphotericin B channel conductance in a concentration-dependent manner. All the effects are likely to be attributed to the influence of the membrane dipole potential on the conductance of single amphotericin B channels. However, specific interactions of some dipole modifiers with polyene-sterol complexes might also contribute to the activity of single amphotericin B pores. It has been shown that the channel dwell time increases with increasing sterol concentration, and it is higher for cholesterol-containing membranes than for bilayers including ergosterol, 6-ketocholestanol, 7-ketocholestanol or 5α-androstan-3β-ol. These findings suggest that the processes of association/dissociation of channel forming molecules depend on the membrane fluidity.
format	article
author	Olga S Ostroumova Svetlana S Efimova Ludmila V Schagina
author_facet	Olga S Ostroumova Svetlana S Efimova Ludmila V Schagina
author_sort	Olga S Ostroumova
title	Probing amphotericin B single channel activity by membrane dipole modifiers.
title_short	Probing amphotericin B single channel activity by membrane dipole modifiers.
title_full	Probing amphotericin B single channel activity by membrane dipole modifiers.
title_fullStr	Probing amphotericin B single channel activity by membrane dipole modifiers.
title_full_unstemmed	Probing amphotericin B single channel activity by membrane dipole modifiers.
title_sort	probing amphotericin b single channel activity by membrane dipole modifiers.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/89a480a8db7442dd8b359875b061c41b
work_keys_str_mv	AT olgasostroumova probingamphotericinbsinglechannelactivitybymembranedipolemodifiers AT svetlanasefimova probingamphotericinbsinglechannelactivitybymembranedipolemodifiers AT ludmilavschagina probingamphotericinbsinglechannelactivitybymembranedipolemodifiers
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Probing amphotericin B single channel activity by membrane dipole modifiers.

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