Functional characterization of the human mariner transposon Hsmar2.

DNA transposons are mobile elements with the ability to mobilize and transport genetic information between different chromosomal loci. Unfortunately, most transposons copies are currently inactivated, little is known about mariner elements in humans despite their role in the evolution of the human g...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Estel Gil, Assumpcio Bosch, David Lampe, Jose M Lizcano, Jose C Perales, Olivier Danos, Miguel Chillon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89aa1b2975ed46929838190c38e31688
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:89aa1b2975ed46929838190c38e31688
record_format	dspace
spelling	oai:doaj.org-article:89aa1b2975ed46929838190c38e316882021-11-18T08:55:49ZFunctional characterization of the human mariner transposon Hsmar2.1932-620310.1371/journal.pone.0073227https://doaj.org/article/89aa1b2975ed46929838190c38e316882013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24039890/?tool=EBIhttps://doaj.org/toc/1932-6203DNA transposons are mobile elements with the ability to mobilize and transport genetic information between different chromosomal loci. Unfortunately, most transposons copies are currently inactivated, little is known about mariner elements in humans despite their role in the evolution of the human genome, even though the Hsmar2 transposon is associated to hotspots for homologous recombination involved in human genetic disorders as Charcot-Marie-Tooth, Prader-Willi/Angelman, and Williams syndromes. This manuscript describes the functional characterization of the human HSMAR2 transposase generated from fossil sequences and shows that the native HSMAR2 is active in human cells, but also in bacteria, with an efficiency similar to other mariner elements. We observe that the sub-cellular localization of HSMAR2 is dependent on the host cell type, and is cytotoxic when overexpressed in HeLa cells. Finally, we also demonstrate that the binding of HSMAR2 to its own ITRs is specific, and that the excision reaction leaves non-canonical footprints both in bacteria and eukaryotic cells.Estel GilAssumpcio BoschDavid LampeJose M LizcanoJose C PeralesOlivier DanosMiguel ChillonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73227 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Estel Gil Assumpcio Bosch David Lampe Jose M Lizcano Jose C Perales Olivier Danos Miguel Chillon Functional characterization of the human mariner transposon Hsmar2.
description	DNA transposons are mobile elements with the ability to mobilize and transport genetic information between different chromosomal loci. Unfortunately, most transposons copies are currently inactivated, little is known about mariner elements in humans despite their role in the evolution of the human genome, even though the Hsmar2 transposon is associated to hotspots for homologous recombination involved in human genetic disorders as Charcot-Marie-Tooth, Prader-Willi/Angelman, and Williams syndromes. This manuscript describes the functional characterization of the human HSMAR2 transposase generated from fossil sequences and shows that the native HSMAR2 is active in human cells, but also in bacteria, with an efficiency similar to other mariner elements. We observe that the sub-cellular localization of HSMAR2 is dependent on the host cell type, and is cytotoxic when overexpressed in HeLa cells. Finally, we also demonstrate that the binding of HSMAR2 to its own ITRs is specific, and that the excision reaction leaves non-canonical footprints both in bacteria and eukaryotic cells.
format	article
author	Estel Gil Assumpcio Bosch David Lampe Jose M Lizcano Jose C Perales Olivier Danos Miguel Chillon
author_facet	Estel Gil Assumpcio Bosch David Lampe Jose M Lizcano Jose C Perales Olivier Danos Miguel Chillon
author_sort	Estel Gil
title	Functional characterization of the human mariner transposon Hsmar2.
title_short	Functional characterization of the human mariner transposon Hsmar2.
title_full	Functional characterization of the human mariner transposon Hsmar2.
title_fullStr	Functional characterization of the human mariner transposon Hsmar2.
title_full_unstemmed	Functional characterization of the human mariner transposon Hsmar2.
title_sort	functional characterization of the human mariner transposon hsmar2.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/89aa1b2975ed46929838190c38e31688
work_keys_str_mv	AT estelgil functionalcharacterizationofthehumanmarinertransposonhsmar2 AT assumpciobosch functionalcharacterizationofthehumanmarinertransposonhsmar2 AT davidlampe functionalcharacterizationofthehumanmarinertransposonhsmar2 AT josemlizcano functionalcharacterizationofthehumanmarinertransposonhsmar2 AT josecperales functionalcharacterizationofthehumanmarinertransposonhsmar2 AT olivierdanos functionalcharacterizationofthehumanmarinertransposonhsmar2 AT miguelchillon functionalcharacterizationofthehumanmarinertransposonhsmar2
_version_	1718421152069582848

Functional characterization of the human mariner transposon Hsmar2.

Ejemplares similares