Controlling for Contaminants in Low-Biomass 16S rRNA Gene Sequencing Experiments

ABSTRACT Microbial communities are commonly studied using culture-independent methods, such as 16S rRNA gene sequencing. However, one challenge in accurately characterizing microbial communities is exogenous bacterial DNA contamination, particularly in low-microbial-biomass niches. Computational app...

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Autores principales: Lisa Karstens, Mark Asquith, Sean Davin, Damien Fair, W. Thomas Gregory, Alan J. Wolfe, Jonathan Braun, Shannon McWeeney
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2019
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Acceso en línea:https://doaj.org/article/89ae3b9ffc60409b9f8dc40bafba3212
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