Key biomarkers within the colorectal cancer related inflammatory microenvironment

Abstract Therapeutic approaches focused on the inflammatory microenvironment are currently gaining more support, as biomolecules involved in the inflammatory colorectal cancer (CRC) tumor microenvironment are being explored. We analyzed tumor and paired normal tissue samples from CRC patients (n = 2...

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Autores principales:	Valentin Calu, Adriana Ionescu, Loredana Stanca, Ovidiu Ionut Geicu, Florin Iordache, Aurelia Magdalena Pisoschi, Andreea Iren Serban, Liviu Bilteanu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89b0d65bf69e4fa8ac063c617d7c2fa2
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spelling	oai:doaj.org-article:89b0d65bf69e4fa8ac063c617d7c2fa22021-12-02T14:26:25ZKey biomarkers within the colorectal cancer related inflammatory microenvironment10.1038/s41598-021-86941-52045-2322https://doaj.org/article/89b0d65bf69e4fa8ac063c617d7c2fa22021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86941-5https://doaj.org/toc/2045-2322Abstract Therapeutic approaches focused on the inflammatory microenvironment are currently gaining more support, as biomolecules involved in the inflammatory colorectal cancer (CRC) tumor microenvironment are being explored. We analyzed tumor and paired normal tissue samples from CRC patients (n = 22) whom underwent tumor resection surgery. We assessed 39 inflammation-involved biomolecules (multiplex magnetic bead-based immunoassay), CEA and CA19-9 (ELISA assay) and the tissue expression levels of occludin and also pErk, STAT1 and STAT3 transcriptional factors (western blot). Tumor staging has been established by histopathological evaluation of HE stained tumor tissue sections. We report 32 biomarkers displaying statistically significant differences in tumor vs. control. Additionally, positive statistical biomarker correlations were found between MMP2–IL8 and BAFF–IL8 (Pearson correlation coefficients > 0.751), while APRIL–MMP2, APRIL–BAFF and APRIL–IL8 were negatively correlated (correlation coefficients < − 0.650). While APRIL, BAFF, IL8 and MMP2 did not modulate with tumor stage, they were inversely related to the immune infiltrate level and CD163 tissue expression. We conclude that the significantly decreased APRIL and increased BAFF, IL8 and MMP2 expression were tumor-specific and deserve consideration in the development of new treatments. Also, the positive correlation between Chitinase 3-like 1 and IL8 (0.57) or MMP2 (0.50) suggest a role in tumor growth and metastasis pathways.Valentin CaluAdriana IonescuLoredana StancaOvidiu Ionut GeicuFlorin IordacheAurelia Magdalena PisoschiAndreea Iren SerbanLiviu BilteanuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentin Calu Adriana Ionescu Loredana Stanca Ovidiu Ionut Geicu Florin Iordache Aurelia Magdalena Pisoschi Andreea Iren Serban Liviu Bilteanu Key biomarkers within the colorectal cancer related inflammatory microenvironment
description	Abstract Therapeutic approaches focused on the inflammatory microenvironment are currently gaining more support, as biomolecules involved in the inflammatory colorectal cancer (CRC) tumor microenvironment are being explored. We analyzed tumor and paired normal tissue samples from CRC patients (n = 22) whom underwent tumor resection surgery. We assessed 39 inflammation-involved biomolecules (multiplex magnetic bead-based immunoassay), CEA and CA19-9 (ELISA assay) and the tissue expression levels of occludin and also pErk, STAT1 and STAT3 transcriptional factors (western blot). Tumor staging has been established by histopathological evaluation of HE stained tumor tissue sections. We report 32 biomarkers displaying statistically significant differences in tumor vs. control. Additionally, positive statistical biomarker correlations were found between MMP2–IL8 and BAFF–IL8 (Pearson correlation coefficients > 0.751), while APRIL–MMP2, APRIL–BAFF and APRIL–IL8 were negatively correlated (correlation coefficients < − 0.650). While APRIL, BAFF, IL8 and MMP2 did not modulate with tumor stage, they were inversely related to the immune infiltrate level and CD163 tissue expression. We conclude that the significantly decreased APRIL and increased BAFF, IL8 and MMP2 expression were tumor-specific and deserve consideration in the development of new treatments. Also, the positive correlation between Chitinase 3-like 1 and IL8 (0.57) or MMP2 (0.50) suggest a role in tumor growth and metastasis pathways.
format	article
author	Valentin Calu Adriana Ionescu Loredana Stanca Ovidiu Ionut Geicu Florin Iordache Aurelia Magdalena Pisoschi Andreea Iren Serban Liviu Bilteanu
author_facet	Valentin Calu Adriana Ionescu Loredana Stanca Ovidiu Ionut Geicu Florin Iordache Aurelia Magdalena Pisoschi Andreea Iren Serban Liviu Bilteanu
author_sort	Valentin Calu
title	Key biomarkers within the colorectal cancer related inflammatory microenvironment
title_short	Key biomarkers within the colorectal cancer related inflammatory microenvironment
title_full	Key biomarkers within the colorectal cancer related inflammatory microenvironment
title_fullStr	Key biomarkers within the colorectal cancer related inflammatory microenvironment
title_full_unstemmed	Key biomarkers within the colorectal cancer related inflammatory microenvironment
title_sort	key biomarkers within the colorectal cancer related inflammatory microenvironment
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/89b0d65bf69e4fa8ac063c617d7c2fa2
work_keys_str_mv	AT valentincalu keybiomarkerswithinthecolorectalcancerrelatedinflammatorymicroenvironment AT adrianaionescu keybiomarkerswithinthecolorectalcancerrelatedinflammatorymicroenvironment AT loredanastanca keybiomarkerswithinthecolorectalcancerrelatedinflammatorymicroenvironment AT ovidiuionutgeicu keybiomarkerswithinthecolorectalcancerrelatedinflammatorymicroenvironment AT floriniordache keybiomarkerswithinthecolorectalcancerrelatedinflammatorymicroenvironment AT aureliamagdalenapisoschi keybiomarkerswithinthecolorectalcancerrelatedinflammatorymicroenvironment AT andreeairenserban keybiomarkerswithinthecolorectalcancerrelatedinflammatorymicroenvironment AT liviubilteanu keybiomarkerswithinthecolorectalcancerrelatedinflammatorymicroenvironment
_version_	1718391323669561344

Key biomarkers within the colorectal cancer related inflammatory microenvironment

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