4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes
We designed and synthesized RNAs containing 4′-C-aminoethoxy-2′-O-methyluridine (4′-AEoU), and compared their properties with those containing 4′-C-aminopropyl-2′-O-methyluridine (4′-APU). The RNA duplexes containing 4′-AEoU showed higher melting temperatures than those containing 4′-APU. The 4′-AEo...
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Autores principales: | , , , , |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/89b5d49529b4487bb53b1ebe44d6c8cf |
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Sumario: | We designed and synthesized RNAs containing 4′-C-aminoethoxy-2′-O-methyluridine (4′-AEoU), and compared their properties with those containing 4′-C-aminopropyl-2′-O-methyluridine (4′-APU). The RNA duplexes containing 4′-AEoU showed higher melting temperatures than those containing 4′-APU. The 4′-AEoU modification enhanced the stability of the RNAs in buffer containing serum. Furthermore, it was found that the incorporation of 4′-AEoU into the passenger strand of small interfering RNAs (siRNAs) is tolerated for the RNA interference (RNAi) activity of the siRNAs. |
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