4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes

We designed and synthesized RNAs containing 4′-C-aminoethoxy-2′-O-methyluridine (4′-AEoU), and compared their properties with those containing 4′-C-aminopropyl-2′-O-methyluridine (4′-APU). The RNA duplexes containing 4′-AEoU showed higher melting temperatures than those containing 4′-APU. The 4′-AEo...

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Autores principales:	Ryo Tsukimura, Ryohei Kajino, Yujun Zhou, Akash Chandela, Yoshihito Ueno
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Oligonucleotide RNA Small interfering RNA Aminoethoxy modification Thermal stability Nuclease resistance Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/89b5d49529b4487bb53b1ebe44d6c8cf
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spelling	oai:doaj.org-article:89b5d49529b4487bb53b1ebe44d6c8cf2021-11-12T04:32:16Z4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes2211-715610.1016/j.rechem.2021.100231https://doaj.org/article/89b5d49529b4487bb53b1ebe44d6c8cf2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211715621001363https://doaj.org/toc/2211-7156We designed and synthesized RNAs containing 4′-C-aminoethoxy-2′-O-methyluridine (4′-AEoU), and compared their properties with those containing 4′-C-aminopropyl-2′-O-methyluridine (4′-APU). The RNA duplexes containing 4′-AEoU showed higher melting temperatures than those containing 4′-APU. The 4′-AEoU modification enhanced the stability of the RNAs in buffer containing serum. Furthermore, it was found that the incorporation of 4′-AEoU into the passenger strand of small interfering RNAs (siRNAs) is tolerated for the RNA interference (RNAi) activity of the siRNAs.Ryo TsukimuraRyohei KajinoYujun ZhouAkash ChandelaYoshihito UenoElsevierarticleOligonucleotideRNASmall interfering RNAAminoethoxy modificationThermal stabilityNuclease resistanceChemistryQD1-999ENResults in Chemistry, Vol 3, Iss , Pp 100231- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Oligonucleotide RNA Small interfering RNA Aminoethoxy modification Thermal stability Nuclease resistance Chemistry QD1-999
spellingShingle	Oligonucleotide RNA Small interfering RNA Aminoethoxy modification Thermal stability Nuclease resistance Chemistry QD1-999 Ryo Tsukimura Ryohei Kajino Yujun Zhou Akash Chandela Yoshihito Ueno 4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes
description	We designed and synthesized RNAs containing 4′-C-aminoethoxy-2′-O-methyluridine (4′-AEoU), and compared their properties with those containing 4′-C-aminopropyl-2′-O-methyluridine (4′-APU). The RNA duplexes containing 4′-AEoU showed higher melting temperatures than those containing 4′-APU. The 4′-AEoU modification enhanced the stability of the RNAs in buffer containing serum. Furthermore, it was found that the incorporation of 4′-AEoU into the passenger strand of small interfering RNAs (siRNAs) is tolerated for the RNA interference (RNAi) activity of the siRNAs.
format	article
author	Ryo Tsukimura Ryohei Kajino Yujun Zhou Akash Chandela Yoshihito Ueno
author_facet	Ryo Tsukimura Ryohei Kajino Yujun Zhou Akash Chandela Yoshihito Ueno
author_sort	Ryo Tsukimura
title	4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes
title_short	4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes
title_full	4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes
title_fullStr	4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes
title_full_unstemmed	4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes
title_sort	4′-c-aminoethoxy modification enhanced nuclease resistance of rnas and improved thermal stability of rna duplexes
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/89b5d49529b4487bb53b1ebe44d6c8cf
work_keys_str_mv	AT ryotsukimura 4caminoethoxymodificationenhancednucleaseresistanceofrnasandimprovedthermalstabilityofrnaduplexes AT ryoheikajino 4caminoethoxymodificationenhancednucleaseresistanceofrnasandimprovedthermalstabilityofrnaduplexes AT yujunzhou 4caminoethoxymodificationenhancednucleaseresistanceofrnasandimprovedthermalstabilityofrnaduplexes AT akashchandela 4caminoethoxymodificationenhancednucleaseresistanceofrnasandimprovedthermalstabilityofrnaduplexes AT yoshihitoueno 4caminoethoxymodificationenhancednucleaseresistanceofrnasandimprovedthermalstabilityofrnaduplexes
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4′-C-Aminoethoxy modification enhanced nuclease resistance of RNAs and improved thermal stability of RNA duplexes

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