Preharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes

‘Honeycrisp’ (<i>Malus domestica</i> Borkh.), a premium applecultivar, is highly susceptible to bitter pit and decline in quality during long-term storage. In order to enhance the quality, an aqueous composition containing hexanal was applied as a preharvest spray. The effects of hexanal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Karthika Sriskantharajah, Walid El Kayal, Murali Mohan Ayyanath, Praveen K. Saxena, Alan J. Sullivan, Gopinadhan Paliyath, Jayasankar Subramanian
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bitter pit calcium sensor proteins ethylene Harvista<sup>TM</sup> hexanal phospholipase D Botany QK1-989
Acceso en línea:	https://doaj.org/article/89b6672991644348926b0687929c055c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:89b6672991644348926b0687929c055c
record_format	dspace
spelling	oai:doaj.org-article:89b6672991644348926b0687929c055c2021-11-25T18:45:26ZPreharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes10.3390/plants101123322223-7747https://doaj.org/article/89b6672991644348926b0687929c055c2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2332https://doaj.org/toc/2223-7747‘Honeycrisp’ (<i>Malus domestica</i> Borkh.), a premium applecultivar, is highly susceptible to bitter pit and decline in quality during long-term storage. In order to enhance the quality, an aqueous composition containing hexanal was applied as a preharvest spray. The effects of hexanal were assessed on the treated fruit and compared with Harvista<sup>TM</sup> (a sprayable 1-Methylcyclopropene based commercial formulation) applied and control fruit under both cold (2.5 °C; four months) and cold after room temperature storage (20 °C; 14 days) conditions. Color, firmness, and total soluble solids (TSS) did not show a significant change in response to any treatment at harvest, while abscisic acid (ABA) significantly reduced and tryptophan increased in response to hexanal, compared to Harvista<sup>TM</sup> and control. The treatment effects on quality traits were observed during storage. Both hexanal and Harvista<sup>TM</sup> sprayed apples had higher TSS under both cold and room temperature storage. In addition, both sprays enhanced firmness at room temperature storage. However, the effects of sprays on other quality traits showed a different pattern. Apples sprayed with hexanal had lower phospholipase D enzyme (PLD) activity, lower incidence of bitter pit, and decreased expression of <i>MdPLDα1</i> compared to Harvista<sup>TM</sup> and control. On the other hand, Harvista<sup>TM</sup> treated fruit produced lower ethylene. Both sprays decreased the expression of <i>MdPLDα4, MdCaM2, MdCaM4</i> and <i>MdCML18</i> genes. Generally, PLD alpha has a direct role in promoting fruit senescence, whereas the calcium senor proteins (CaM/CMLs) may involve in fruit ripening process via calcium and ethylene interactions. Therefore, improved postharvest qualities, including the lower incidence of bitter pit in hexanal treated ‘Honeycrisp’, may be associated with lower membrane damage due to lower PLD enzyme activity and decreased expression of <i>MdPLDα1</i> and <i>MdPLDα4</i> genes throughout the storage period.Karthika SriskantharajahWalid El KayalMurali Mohan AyyanathPraveen K. SaxenaAlan J. SullivanGopinadhan PaliyathJayasankar SubramanianMDPI AGarticlebitter pitcalcium sensor proteinsethyleneHarvista<sup>TM</sup>hexanalphospholipase DBotanyQK1-989ENPlants, Vol 10, Iss 2332, p 2332 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bitter pit calcium sensor proteins ethylene Harvista<sup>TM</sup> hexanal phospholipase D Botany QK1-989
spellingShingle	bitter pit calcium sensor proteins ethylene Harvista<sup>TM</sup> hexanal phospholipase D Botany QK1-989 Karthika Sriskantharajah Walid El Kayal Murali Mohan Ayyanath Praveen K. Saxena Alan J. Sullivan Gopinadhan Paliyath Jayasankar Subramanian Preharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes
description	‘Honeycrisp’ (<i>Malus domestica</i> Borkh.), a premium applecultivar, is highly susceptible to bitter pit and decline in quality during long-term storage. In order to enhance the quality, an aqueous composition containing hexanal was applied as a preharvest spray. The effects of hexanal were assessed on the treated fruit and compared with Harvista<sup>TM</sup> (a sprayable 1-Methylcyclopropene based commercial formulation) applied and control fruit under both cold (2.5 °C; four months) and cold after room temperature storage (20 °C; 14 days) conditions. Color, firmness, and total soluble solids (TSS) did not show a significant change in response to any treatment at harvest, while abscisic acid (ABA) significantly reduced and tryptophan increased in response to hexanal, compared to Harvista<sup>TM</sup> and control. The treatment effects on quality traits were observed during storage. Both hexanal and Harvista<sup>TM</sup> sprayed apples had higher TSS under both cold and room temperature storage. In addition, both sprays enhanced firmness at room temperature storage. However, the effects of sprays on other quality traits showed a different pattern. Apples sprayed with hexanal had lower phospholipase D enzyme (PLD) activity, lower incidence of bitter pit, and decreased expression of <i>MdPLDα1</i> compared to Harvista<sup>TM</sup> and control. On the other hand, Harvista<sup>TM</sup> treated fruit produced lower ethylene. Both sprays decreased the expression of <i>MdPLDα4, MdCaM2, MdCaM4</i> and <i>MdCML18</i> genes. Generally, PLD alpha has a direct role in promoting fruit senescence, whereas the calcium senor proteins (CaM/CMLs) may involve in fruit ripening process via calcium and ethylene interactions. Therefore, improved postharvest qualities, including the lower incidence of bitter pit in hexanal treated ‘Honeycrisp’, may be associated with lower membrane damage due to lower PLD enzyme activity and decreased expression of <i>MdPLDα1</i> and <i>MdPLDα4</i> genes throughout the storage period.
format	article
author	Karthika Sriskantharajah Walid El Kayal Murali Mohan Ayyanath Praveen K. Saxena Alan J. Sullivan Gopinadhan Paliyath Jayasankar Subramanian
author_facet	Karthika Sriskantharajah Walid El Kayal Murali Mohan Ayyanath Praveen K. Saxena Alan J. Sullivan Gopinadhan Paliyath Jayasankar Subramanian
author_sort	Karthika Sriskantharajah
title	Preharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes
title_short	Preharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes
title_full	Preharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes
title_fullStr	Preharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes
title_full_unstemmed	Preharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes
title_sort	preharvest spray hexanal formulation enhances postharvest quality in ‘honeycrisp’ apples by regulating phospholipase d and calcium sensor proteins genes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/89b6672991644348926b0687929c055c
work_keys_str_mv	AT karthikasriskantharajah preharvestsprayhexanalformulationenhancespostharvestqualityinhoneycrispapplesbyregulatingphospholipasedandcalciumsensorproteinsgenes AT walidelkayal preharvestsprayhexanalformulationenhancespostharvestqualityinhoneycrispapplesbyregulatingphospholipasedandcalciumsensorproteinsgenes AT muralimohanayyanath preharvestsprayhexanalformulationenhancespostharvestqualityinhoneycrispapplesbyregulatingphospholipasedandcalciumsensorproteinsgenes AT praveenksaxena preharvestsprayhexanalformulationenhancespostharvestqualityinhoneycrispapplesbyregulatingphospholipasedandcalciumsensorproteinsgenes AT alanjsullivan preharvestsprayhexanalformulationenhancespostharvestqualityinhoneycrispapplesbyregulatingphospholipasedandcalciumsensorproteinsgenes AT gopinadhanpaliyath preharvestsprayhexanalformulationenhancespostharvestqualityinhoneycrispapplesbyregulatingphospholipasedandcalciumsensorproteinsgenes AT jayasankarsubramanian preharvestsprayhexanalformulationenhancespostharvestqualityinhoneycrispapplesbyregulatingphospholipasedandcalciumsensorproteinsgenes
_version_	1718410722995601408

Preharvest Spray Hexanal Formulation Enhances Postharvest Quality in ‘Honeycrisp’ Apples by Regulating Phospholipase D and Calcium Sensor Proteins Genes

Ejemplares similares