Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection

Abstract Neutrophils are innate immune cells involved in the elimination of pathogens and can also induce adaptive immune responses. Nα and Nβ neutrophils have been described with distinct in vitro capacity to generate antigen-specific CD8 T-cell responses. However, how these cell types exert their...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mauro Di Pilato, Miguel Palomino-Segura, Ernesto Mejías-Pérez, Carmen E. Gómez, Andrea Rubio-Ponce, Rocco D’Antuono, Diego Ulisse Pizzagalli, Patricia Pérez, Raphael Kfuri-Rubens, Alberto Benguría, Ana Dopazo, Iván Ballesteros, Carlos Oscar S. Sorzano, Andrés Hidalgo, Mariano Esteban, Santiago F. Gonzalez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/89b84f82228e4bf9963a98f0174b37ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:89b84f82228e4bf9963a98f0174b37ad
record_format	dspace
spelling	oai:doaj.org-article:89b84f82228e4bf9963a98f0174b37ad2021-12-02T14:30:51ZNeutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection10.1038/s41541-021-00314-72059-0105https://doaj.org/article/89b84f82228e4bf9963a98f0174b37ad2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00314-7https://doaj.org/toc/2059-0105Abstract Neutrophils are innate immune cells involved in the elimination of pathogens and can also induce adaptive immune responses. Nα and Nβ neutrophils have been described with distinct in vitro capacity to generate antigen-specific CD8 T-cell responses. However, how these cell types exert their role in vivo and how manipulation of Nβ/Nα ratio influences vaccine-mediated immune responses are not known. In this study, we find that these neutrophil subtypes show distinct migratory and motility patterns and different ability to interact with CD8 T cells in the spleen following vaccinia virus (VACV) infection. Moreover, after analysis of adhesion, inflammatory, and migration markers, we observe that Nβ neutrophils overexpress the α4β1 integrin compared to Nα. Finally, by inhibiting α4β1 integrin, we increase the Nβ/Nα ratio and enhance CD8 T-cell responses to HIV VACV-delivered antigens. These findings provide significant advancements in the comprehension of neutrophil-based control of adaptive immune system and their relevance in vaccine design.Mauro Di PilatoMiguel Palomino-SeguraErnesto Mejías-PérezCarmen E. GómezAndrea Rubio-PonceRocco D’AntuonoDiego Ulisse PizzagalliPatricia PérezRaphael Kfuri-RubensAlberto BenguríaAna DopazoIván BallesterosCarlos Oscar S. SorzanoAndrés HidalgoMariano EstebanSantiago F. GonzalezNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Mauro Di Pilato Miguel Palomino-Segura Ernesto Mejías-Pérez Carmen E. Gómez Andrea Rubio-Ponce Rocco D’Antuono Diego Ulisse Pizzagalli Patricia Pérez Raphael Kfuri-Rubens Alberto Benguría Ana Dopazo Iván Ballesteros Carlos Oscar S. Sorzano Andrés Hidalgo Mariano Esteban Santiago F. Gonzalez Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection
description	Abstract Neutrophils are innate immune cells involved in the elimination of pathogens and can also induce adaptive immune responses. Nα and Nβ neutrophils have been described with distinct in vitro capacity to generate antigen-specific CD8 T-cell responses. However, how these cell types exert their role in vivo and how manipulation of Nβ/Nα ratio influences vaccine-mediated immune responses are not known. In this study, we find that these neutrophil subtypes show distinct migratory and motility patterns and different ability to interact with CD8 T cells in the spleen following vaccinia virus (VACV) infection. Moreover, after analysis of adhesion, inflammatory, and migration markers, we observe that Nβ neutrophils overexpress the α4β1 integrin compared to Nα. Finally, by inhibiting α4β1 integrin, we increase the Nβ/Nα ratio and enhance CD8 T-cell responses to HIV VACV-delivered antigens. These findings provide significant advancements in the comprehension of neutrophil-based control of adaptive immune system and their relevance in vaccine design.
format	article
author	Mauro Di Pilato Miguel Palomino-Segura Ernesto Mejías-Pérez Carmen E. Gómez Andrea Rubio-Ponce Rocco D’Antuono Diego Ulisse Pizzagalli Patricia Pérez Raphael Kfuri-Rubens Alberto Benguría Ana Dopazo Iván Ballesteros Carlos Oscar S. Sorzano Andrés Hidalgo Mariano Esteban Santiago F. Gonzalez
author_facet	Mauro Di Pilato Miguel Palomino-Segura Ernesto Mejías-Pérez Carmen E. Gómez Andrea Rubio-Ponce Rocco D’Antuono Diego Ulisse Pizzagalli Patricia Pérez Raphael Kfuri-Rubens Alberto Benguría Ana Dopazo Iván Ballesteros Carlos Oscar S. Sorzano Andrés Hidalgo Mariano Esteban Santiago F. Gonzalez
author_sort	Mauro Di Pilato
title	Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection
title_short	Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection
title_full	Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection
title_fullStr	Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection
title_full_unstemmed	Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection
title_sort	neutrophil subtypes shape hiv-specific cd8 t-cell responses after vaccinia virus infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/89b84f82228e4bf9963a98f0174b37ad
work_keys_str_mv	AT maurodipilato neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT miguelpalominosegura neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT ernestomejiasperez neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT carmenegomez neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT andrearubioponce neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT roccodantuono neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT diegoulissepizzagalli neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT patriciaperez neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT raphaelkfurirubens neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT albertobenguria neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT anadopazo neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT ivanballesteros neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT carlososcarssorzano neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT andreshidalgo neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT marianoesteban neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection AT santiagofgonzalez neutrophilsubtypesshapehivspecificcd8tcellresponsesaftervacciniavirusinfection
_version_	1718391204188520448

Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection

Ejemplares similares