Icariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy

Yuelong Huang,1 Rui Shi,2 Min Gong,3 Jingshuang Zhang,2 Weiyang Li,2 Qingpeng Song,1 Chengai Wu,2 Wei Tian1 1Department of Spine Surgery of Beijing Jishuitan Hospital, The Fourth Clinical Medical College of Peking University, Beijing 100035, China; 2Institute of Traumatology and Orthopaedics, Beiji...

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Autores principales:	Huang YL, Shi R, Gong M, Zhang JS, Li WY, Song QP, Wu CA, Tian W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Icariin Polycaprolactone /gelatin Electrospun Epidural adhesion Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/89bdecbb5f61497f8ed28083482e5b53
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spelling	oai:doaj.org-article:89bdecbb5f61497f8ed28083482e5b532021-12-02T08:57:25ZIcariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy1178-2013https://doaj.org/article/89bdecbb5f61497f8ed28083482e5b532018-08-01T00:00:00Zhttps://www.dovepress.com/icariin-loaded-electrospun-pclgelatin-sub-microfiber-mat-for-preventin-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yuelong Huang,1 Rui Shi,2 Min Gong,3 Jingshuang Zhang,2 Weiyang Li,2 Qingpeng Song,1 Chengai Wu,2 Wei Tian1 1Department of Spine Surgery of Beijing Jishuitan Hospital, The Fourth Clinical Medical College of Peking University, Beijing 100035, China; 2Institute of Traumatology and Orthopaedics, Beijing Laboratory of Biomedical Materials, Beijing Jishuitan Hospital, Beijing 100035, China; 3Beijing Laboratory of Biomedical Materials, State Key Laboratory of Organic–Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China Background: Epidural adhesion is one of the major reasons attributed to failed back surgery syndrome after a successful laminectomy, and results in serious clinical complications which require management from physicians. Therefore, there is an urgent demand within the field to develop biodegradable anti-adhesion membranes for the prevention of post-operative adhesion. Methods: In this study, icariin (ICA) was initially loaded into polycaprolactone (PCL)/gelatin fibers via electrospinning to fabricate nanofibrous membranes. The effects of the ICA content (0.5wt%, 2wt% and 5wt%) and the bioactivity of ICA in the nanofibrous membranes were investigated in vitro and in vivo. Results: The nanofibrous membranes showed suitable pore size and good properties that were unaffected by ICA concentration. Moreover, the ICA-loaded membranes exhibited an originally rapid and subsequently gradual sustained ICA release profile that could significantly prevent fibroblast adhesion and proliferation. In vivo studies with rabbit laminectomy models demonstrated that the ICA-loaded membranes effectively reduced epidural adhesion by gross observation, histology, and biochemical evaluation. The anti-adhesion mechanism of ICA was found to be via suppression of the TGF-β/Smad signaling proteins and down regulation of collage I/III and a-SMA expression for the first time. Conclusion: We believe that these ICA-loaded PCL/gelatin electrospun membranes provide a novel and promising strategy to resist adhesion formation following laminectomy in a clinical application. Keywords: icariin, polycaprolactone/gelatin, electrospun, epidural adhesionHuang YLShi RGong MZhang JSLi WYSong QPWu CATian WDove Medical PressarticleIcariinPolycaprolactone /gelatinElectrospunEpidural adhesionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4831-4844 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Icariin Polycaprolactone /gelatin Electrospun Epidural adhesion Medicine (General) R5-920
spellingShingle	Icariin Polycaprolactone /gelatin Electrospun Epidural adhesion Medicine (General) R5-920 Huang YL Shi R Gong M Zhang JS Li WY Song QP Wu CA Tian W Icariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy
description	Yuelong Huang,1 Rui Shi,2 Min Gong,3 Jingshuang Zhang,2 Weiyang Li,2 Qingpeng Song,1 Chengai Wu,2 Wei Tian1 1Department of Spine Surgery of Beijing Jishuitan Hospital, The Fourth Clinical Medical College of Peking University, Beijing 100035, China; 2Institute of Traumatology and Orthopaedics, Beijing Laboratory of Biomedical Materials, Beijing Jishuitan Hospital, Beijing 100035, China; 3Beijing Laboratory of Biomedical Materials, State Key Laboratory of Organic–Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China Background: Epidural adhesion is one of the major reasons attributed to failed back surgery syndrome after a successful laminectomy, and results in serious clinical complications which require management from physicians. Therefore, there is an urgent demand within the field to develop biodegradable anti-adhesion membranes for the prevention of post-operative adhesion. Methods: In this study, icariin (ICA) was initially loaded into polycaprolactone (PCL)/gelatin fibers via electrospinning to fabricate nanofibrous membranes. The effects of the ICA content (0.5wt%, 2wt% and 5wt%) and the bioactivity of ICA in the nanofibrous membranes were investigated in vitro and in vivo. Results: The nanofibrous membranes showed suitable pore size and good properties that were unaffected by ICA concentration. Moreover, the ICA-loaded membranes exhibited an originally rapid and subsequently gradual sustained ICA release profile that could significantly prevent fibroblast adhesion and proliferation. In vivo studies with rabbit laminectomy models demonstrated that the ICA-loaded membranes effectively reduced epidural adhesion by gross observation, histology, and biochemical evaluation. The anti-adhesion mechanism of ICA was found to be via suppression of the TGF-β/Smad signaling proteins and down regulation of collage I/III and a-SMA expression for the first time. Conclusion: We believe that these ICA-loaded PCL/gelatin electrospun membranes provide a novel and promising strategy to resist adhesion formation following laminectomy in a clinical application. Keywords: icariin, polycaprolactone/gelatin, electrospun, epidural adhesion
format	article
author	Huang YL Shi R Gong M Zhang JS Li WY Song QP Wu CA Tian W
author_facet	Huang YL Shi R Gong M Zhang JS Li WY Song QP Wu CA Tian W
author_sort	Huang YL
title	Icariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy
title_short	Icariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy
title_full	Icariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy
title_fullStr	Icariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy
title_full_unstemmed	Icariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy
title_sort	icariin-loaded electrospun pcl/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/89bdecbb5f61497f8ed28083482e5b53
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Icariin-loaded electrospun PCL/gelatin sub-microfiber mat for preventing epidural adhesions after laminectomy

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