Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.

HP1 is a major component of chromatin and regulates gene expression through its binding to methylated histone H3. Most eukaryotes express at least three isoforms of HP1 with similar domain architecture. However, despite the common specificity for methylated histone H3, the three HP1 isoforms bind to...

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Autores principales:	Jochen Abel, Ragnhild Eskeland, Grazia D Raffa, Elisabeth Kremmer, Axel Imhof
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89c9d7ccabd84b1db8d6fc50baf83cde
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spelling	oai:doaj.org-article:89c9d7ccabd84b1db8d6fc50baf83cde2021-11-25T06:16:17ZDrosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.1932-620310.1371/journal.pone.0005089https://doaj.org/article/89c9d7ccabd84b1db8d6fc50baf83cde2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19352434/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203HP1 is a major component of chromatin and regulates gene expression through its binding to methylated histone H3. Most eukaryotes express at least three isoforms of HP1 with similar domain architecture. However, despite the common specificity for methylated histone H3, the three HP1 isoforms bind to different regions of the genome. Most of the studies so far focused on the HP1a isoform and its role in transcriptional regulation. As HP1a requires additional factors to bind methylated chromatin in vitro, we wondered whether another isoform might also require additional targeting factors. Indeed, we found that HP1c interacts with the DNA binding factors Woc and Row and requires Woc to become targeted to chromatin in vivo. Moreover, we show that the interaction between HP1c and Woc constitutes a transcriptional feedback loop that operates to balance the concentration of HP1c within the cell. This regulation may prevent HP1c from binding to methylated heterochromatin.Jochen AbelRagnhild EskelandGrazia D RaffaElisabeth KremmerAxel ImhofPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e5089 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jochen Abel Ragnhild Eskeland Grazia D Raffa Elisabeth Kremmer Axel Imhof Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.
description	HP1 is a major component of chromatin and regulates gene expression through its binding to methylated histone H3. Most eukaryotes express at least three isoforms of HP1 with similar domain architecture. However, despite the common specificity for methylated histone H3, the three HP1 isoforms bind to different regions of the genome. Most of the studies so far focused on the HP1a isoform and its role in transcriptional regulation. As HP1a requires additional factors to bind methylated chromatin in vitro, we wondered whether another isoform might also require additional targeting factors. Indeed, we found that HP1c interacts with the DNA binding factors Woc and Row and requires Woc to become targeted to chromatin in vivo. Moreover, we show that the interaction between HP1c and Woc constitutes a transcriptional feedback loop that operates to balance the concentration of HP1c within the cell. This regulation may prevent HP1c from binding to methylated heterochromatin.
format	article
author	Jochen Abel Ragnhild Eskeland Grazia D Raffa Elisabeth Kremmer Axel Imhof
author_facet	Jochen Abel Ragnhild Eskeland Grazia D Raffa Elisabeth Kremmer Axel Imhof
author_sort	Jochen Abel
title	Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.
title_short	Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.
title_full	Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.
title_fullStr	Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.
title_full_unstemmed	Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.
title_sort	drosophila hp1c is regulated by an auto-regulatory feedback loop through its binding partner woc.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/89c9d7ccabd84b1db8d6fc50baf83cde
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Drosophila HP1c is regulated by an auto-regulatory feedback loop through its binding partner Woc.

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