Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases

Summary: Early steps of cancer initiation and metastasis, while critical for understanding disease mechanisms, are difficult to visualize and study. Here, we describe an approach to study the processes of initiation, progression, and metastasis of prostate cancer (PC) in a genetically engineered Rap...

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Autores principales:	Julian Taranda, Grinu Mathew, Kaitlin Watrud, Nour El-Amine, Matthew F. Lee, Corey Elowsky, Anastasiia Bludova, Sintia Escobar Avelar, Dawid G. Nowak, Tse-Luen Wee, John E. Wilkinson, Lloyd C. Trotman, Pavel Osten
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	prostate cancer serial two-photon tomography whole-organ imaging early metastasis single-cell resolution Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/89cbad6d853b4185be074d68ba31ccb4
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spelling	oai:doaj.org-article:89cbad6d853b4185be074d68ba31ccb42021-11-18T04:48:03ZCombined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases2211-124710.1016/j.celrep.2021.110027https://doaj.org/article/89cbad6d853b4185be074d68ba31ccb42021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211124721015096https://doaj.org/toc/2211-1247Summary: Early steps of cancer initiation and metastasis, while critical for understanding disease mechanisms, are difficult to visualize and study. Here, we describe an approach to study the processes of initiation, progression, and metastasis of prostate cancer (PC) in a genetically engineered RapidCaP mouse model, which combines whole-organ imaging by serial two-photon tomography (STPT) and post hoc thick-section immunofluorescent (IF) analysis. STPT enables the detection of single tumor-initiating cells within the entire prostate, and consequent IF analysis reveals a transition from normal to transformed epithelial tissue and cell escape from the tumor focus. STPT imaging of the liver and brain reveal the distribution of multiple metastatic foci in the liver and an early-stage metastatic cell invasion in the brain. This imaging and data analysis pipeline can be readily applied to other mouse models of cancer, offering a highly versatile whole-organ platform to study in situ mechanisms of cancer initiation and progression.Julian TarandaGrinu MathewKaitlin WatrudNour El-AmineMatthew F. LeeCorey ElowskyAnastasiia BludovaSintia Escobar AvelarDawid G. NowakTse-Luen WeeJohn E. WilkinsonLloyd C. TrotmanPavel OstenElsevierarticleprostate cancerserial two-photon tomographywhole-organ imagingearly metastasissingle-cell resolutionBiology (General)QH301-705.5ENCell Reports, Vol 37, Iss 7, Pp 110027- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	prostate cancer serial two-photon tomography whole-organ imaging early metastasis single-cell resolution Biology (General) QH301-705.5
spellingShingle	prostate cancer serial two-photon tomography whole-organ imaging early metastasis single-cell resolution Biology (General) QH301-705.5 Julian Taranda Grinu Mathew Kaitlin Watrud Nour El-Amine Matthew F. Lee Corey Elowsky Anastasiia Bludova Sintia Escobar Avelar Dawid G. Nowak Tse-Luen Wee John E. Wilkinson Lloyd C. Trotman Pavel Osten Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
description	Summary: Early steps of cancer initiation and metastasis, while critical for understanding disease mechanisms, are difficult to visualize and study. Here, we describe an approach to study the processes of initiation, progression, and metastasis of prostate cancer (PC) in a genetically engineered RapidCaP mouse model, which combines whole-organ imaging by serial two-photon tomography (STPT) and post hoc thick-section immunofluorescent (IF) analysis. STPT enables the detection of single tumor-initiating cells within the entire prostate, and consequent IF analysis reveals a transition from normal to transformed epithelial tissue and cell escape from the tumor focus. STPT imaging of the liver and brain reveal the distribution of multiple metastatic foci in the liver and an early-stage metastatic cell invasion in the brain. This imaging and data analysis pipeline can be readily applied to other mouse models of cancer, offering a highly versatile whole-organ platform to study in situ mechanisms of cancer initiation and progression.
format	article
author	Julian Taranda Grinu Mathew Kaitlin Watrud Nour El-Amine Matthew F. Lee Corey Elowsky Anastasiia Bludova Sintia Escobar Avelar Dawid G. Nowak Tse-Luen Wee John E. Wilkinson Lloyd C. Trotman Pavel Osten
author_facet	Julian Taranda Grinu Mathew Kaitlin Watrud Nour El-Amine Matthew F. Lee Corey Elowsky Anastasiia Bludova Sintia Escobar Avelar Dawid G. Nowak Tse-Luen Wee John E. Wilkinson Lloyd C. Trotman Pavel Osten
author_sort	Julian Taranda
title	Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
title_short	Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
title_full	Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
title_fullStr	Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
title_full_unstemmed	Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
title_sort	combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/89cbad6d853b4185be074d68ba31ccb4
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Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases

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