Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.

Isoprenoids, which are a large group of natural and chemical compounds with a variety of applications as e.g. fragrances, pharmaceuticals and potential biofuels, are produced via two different metabolic pathways, the mevalonate (MVA) pathway and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway....

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Autores principales:	Siavash Partow, Verena Siewers, Laurent Daviet, Michel Schalk, Jens Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89ee30ee2a284ea4acadc4de6974e029
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spelling	oai:doaj.org-article:89ee30ee2a284ea4acadc4de6974e0292021-11-18T08:03:20ZReconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0052498https://doaj.org/article/89ee30ee2a284ea4acadc4de6974e0292012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285068/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Isoprenoids, which are a large group of natural and chemical compounds with a variety of applications as e.g. fragrances, pharmaceuticals and potential biofuels, are produced via two different metabolic pathways, the mevalonate (MVA) pathway and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Here, we attempted to replace the endogenous MVA pathway in Saccharomyces cerevisiae by a synthetic bacterial MEP pathway integrated into the genome to benefit from its superior properties in terms of energy consumption and productivity at defined growth conditions. It was shown that the growth of a MVA pathway deficient S. cerevisiae strain could not be restored by the heterologous MEP pathway even when accompanied by the co-expression of genes erpA, hISCA1 and CpIscA involved in the Fe-S trafficking routes leading to maturation of IspG and IspH and E. coli genes fldA and fpr encoding flavodoxin and flavodoxin reductase believed to be responsible for electron transfer to IspG and IspH.Siavash PartowVerena SiewersLaurent DavietMichel SchalkJens NielsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52498 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Siavash Partow Verena Siewers Laurent Daviet Michel Schalk Jens Nielsen Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.
description	Isoprenoids, which are a large group of natural and chemical compounds with a variety of applications as e.g. fragrances, pharmaceuticals and potential biofuels, are produced via two different metabolic pathways, the mevalonate (MVA) pathway and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Here, we attempted to replace the endogenous MVA pathway in Saccharomyces cerevisiae by a synthetic bacterial MEP pathway integrated into the genome to benefit from its superior properties in terms of energy consumption and productivity at defined growth conditions. It was shown that the growth of a MVA pathway deficient S. cerevisiae strain could not be restored by the heterologous MEP pathway even when accompanied by the co-expression of genes erpA, hISCA1 and CpIscA involved in the Fe-S trafficking routes leading to maturation of IspG and IspH and E. coli genes fldA and fpr encoding flavodoxin and flavodoxin reductase believed to be responsible for electron transfer to IspG and IspH.
format	article
author	Siavash Partow Verena Siewers Laurent Daviet Michel Schalk Jens Nielsen
author_facet	Siavash Partow Verena Siewers Laurent Daviet Michel Schalk Jens Nielsen
author_sort	Siavash Partow
title	Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.
title_short	Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.
title_full	Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.
title_fullStr	Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.
title_full_unstemmed	Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.
title_sort	reconstruction and evaluation of the synthetic bacterial mep pathway in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/89ee30ee2a284ea4acadc4de6974e029
work_keys_str_mv	AT siavashpartow reconstructionandevaluationofthesyntheticbacterialmeppathwayinsaccharomycescerevisiae AT verenasiewers reconstructionandevaluationofthesyntheticbacterialmeppathwayinsaccharomycescerevisiae AT laurentdaviet reconstructionandevaluationofthesyntheticbacterialmeppathwayinsaccharomycescerevisiae AT michelschalk reconstructionandevaluationofthesyntheticbacterialmeppathwayinsaccharomycescerevisiae AT jensnielsen reconstructionandevaluationofthesyntheticbacterialmeppathwayinsaccharomycescerevisiae
_version_	1718422614994583552

Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae.

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