Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients

Abstract Effective implementation of antibiotic stewardship, especially in critical care, is limited by a lack of direct comparative investigations on how different antibiotics impact the microbiota and antibiotic resistance rates. We investigated the impact of two commonly used antibiotics, third-g...

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Autores principales:	Hasinika K. A. H. Gamage, Carola Venturini, Sasha G. Tetu, Masrura Kabir, Vineet Nayyar, Andrew N. Ginn, Belinda Roychoudhry, Lee Thomas, Mitchell Brown, Andrew Holmes, Sally R. Partridge, Ian Seppelt, Ian T. Paulsen, Jonathan R. Iredell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89ef1323acf544d9a6dfaafa7d3554be
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spelling	oai:doaj.org-article:89ef1323acf544d9a6dfaafa7d3554be2021-12-02T14:25:21ZThird generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients10.1038/s41598-021-85946-42045-2322https://doaj.org/article/89ef1323acf544d9a6dfaafa7d3554be2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85946-4https://doaj.org/toc/2045-2322Abstract Effective implementation of antibiotic stewardship, especially in critical care, is limited by a lack of direct comparative investigations on how different antibiotics impact the microbiota and antibiotic resistance rates. We investigated the impact of two commonly used antibiotics, third-generation cephalosporins (3GC) and piperacillin/tazobactam (TZP) on the endotracheal, perineal and faecal microbiota of intensive care patients in Australia. Patients exposed to either 3GC, TZP, or no β-lactams (control group) were sampled over time and 16S rRNA amplicon sequencing was performed to examine microbiota diversity and composition. While neither treatment significantly affected diversity, numerous changes to microbiota composition were associated with each treatment. The shifts in microbiota composition associated with 3GC exposure differed from those observed with TZP, consistent with previous reports in animal models. This included a significant increase in Enterobacteriaceae and Enterococcaceae abundance in endotracheal and perineal microbiota for those administered 3GC compared to the control group. Culture-based analyses did not identify any significant changes in the prevalence of specific pathogenic or antibiotic-resistant bacteria. Exposure to clinical antibiotics has previously been linked to reduced microbiota diversity and increased antimicrobial resistance, but our results indicate that these effects may not be immediately apparent after short-term real-world exposures.Hasinika K. A. H. GamageCarola VenturiniSasha G. TetuMasrura KabirVineet NayyarAndrew N. GinnBelinda RoychoudhryLee ThomasMitchell BrownAndrew HolmesSally R. PartridgeIan SeppeltIan T. PaulsenJonathan R. IredellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hasinika K. A. H. Gamage Carola Venturini Sasha G. Tetu Masrura Kabir Vineet Nayyar Andrew N. Ginn Belinda Roychoudhry Lee Thomas Mitchell Brown Andrew Holmes Sally R. Partridge Ian Seppelt Ian T. Paulsen Jonathan R. Iredell Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients
description	Abstract Effective implementation of antibiotic stewardship, especially in critical care, is limited by a lack of direct comparative investigations on how different antibiotics impact the microbiota and antibiotic resistance rates. We investigated the impact of two commonly used antibiotics, third-generation cephalosporins (3GC) and piperacillin/tazobactam (TZP) on the endotracheal, perineal and faecal microbiota of intensive care patients in Australia. Patients exposed to either 3GC, TZP, or no β-lactams (control group) were sampled over time and 16S rRNA amplicon sequencing was performed to examine microbiota diversity and composition. While neither treatment significantly affected diversity, numerous changes to microbiota composition were associated with each treatment. The shifts in microbiota composition associated with 3GC exposure differed from those observed with TZP, consistent with previous reports in animal models. This included a significant increase in Enterobacteriaceae and Enterococcaceae abundance in endotracheal and perineal microbiota for those administered 3GC compared to the control group. Culture-based analyses did not identify any significant changes in the prevalence of specific pathogenic or antibiotic-resistant bacteria. Exposure to clinical antibiotics has previously been linked to reduced microbiota diversity and increased antimicrobial resistance, but our results indicate that these effects may not be immediately apparent after short-term real-world exposures.
format	article
author	Hasinika K. A. H. Gamage Carola Venturini Sasha G. Tetu Masrura Kabir Vineet Nayyar Andrew N. Ginn Belinda Roychoudhry Lee Thomas Mitchell Brown Andrew Holmes Sally R. Partridge Ian Seppelt Ian T. Paulsen Jonathan R. Iredell
author_facet	Hasinika K. A. H. Gamage Carola Venturini Sasha G. Tetu Masrura Kabir Vineet Nayyar Andrew N. Ginn Belinda Roychoudhry Lee Thomas Mitchell Brown Andrew Holmes Sally R. Partridge Ian Seppelt Ian T. Paulsen Jonathan R. Iredell
author_sort	Hasinika K. A. H. Gamage
title	Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients
title_short	Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients
title_full	Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients
title_fullStr	Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients
title_full_unstemmed	Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients
title_sort	third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/89ef1323acf544d9a6dfaafa7d3554be
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Third generation cephalosporins and piperacillin/tazobactam have distinct impacts on the microbiota of critically ill patients

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