Pathogenicity and Volatile Nematicidal Metabolites from Duddingtonia flagrans against Meloidogyne incognita

Plant parasitic nematodes, especially parasitic root-knot nematodes, are one of the most destructive plant pathogens worldwide. The control of plant root-knot nematodes is extremely challenging. Duddingtonia flagrans is a type of nematode-trapping fungi (NTF), which produces three...

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Autores principales:	Xiaoyu Mei, Xin Wang, Guohong Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nematode-trapping fungi <i>Duddingtonia flagrans</i> <i>Meloidogyne incognita</i> VOCs cyclohexanamine egg hatching Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/89eff4ea375d460d86738bc5a05b31f1
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spelling	oai:doaj.org-article:89eff4ea375d460d86738bc5a05b31f12021-11-25T18:24:45ZPathogenicity and Volatile Nematicidal Metabolites from <i>Duddingtonia flagrans</i> against <i>Meloidogyne incognita</i>10.3390/microorganisms91122682076-2607https://doaj.org/article/89eff4ea375d460d86738bc5a05b31f12021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2268https://doaj.org/toc/2076-2607Plant parasitic nematodes, especially parasitic root-knot nematodes, are one of the most destructive plant pathogens worldwide. The control of plant root-knot nematodes is extremely challenging. <i>Duddingtonia flagrans</i> is a type of nematode-trapping fungi (NTF), which produces three-dimensional adhesive networks to trap nematodes. In this study, the pathogenicity and volatile organic compounds (VOCs) of the NTF <i>D. flagrans</i> against the plant root-knot nematode, <i>Meloidogyne incognita</i>, were investigated. The predatory process of <i>D. flagrans</i> trapping <i>M. incognita</i> was observed using scanning electron microscopy. Gas chromatography-mass spectrometry analysis of the VOCs from <i>D. flagrans</i> led to the identification of 52 metabolites, of which 11 main compounds were tested individually for their activity against <i>M. incognita</i>. Three compounds, cyclohexanamine, cyclohexanone, and cyclohexanol, were toxic to <i>M. incognita</i>. Furthermore, these three VOCs inhibited egg hatching of <i>M. incognita</i>. Cyclohexanamine showed the highest nematicidal activity, which can cause 97.93% mortality of <i>M. incognita</i> at 8.71 µM within 12 h. The number of hatched juveniles per egg mass after 3 days was just 8.44 when treated with 26.14 µM cyclohexanamine. This study is the first to demonstrate the nematicidal activity of VOCs produced by <i>D. flagrans</i> against <i>M. incognita</i>, which indicates that <i>D. flagrans</i> has the potential to biocontrol plant root-knot nematodes.Xiaoyu MeiXin WangGuohong LiMDPI AGarticlenematode-trapping fungi<i>Duddingtonia flagrans</i><i>Meloidogyne incognita</i>VOCscyclohexanamineegg hatchingBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2268, p 2268 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nematode-trapping fungi <i>Duddingtonia flagrans</i> <i>Meloidogyne incognita</i> VOCs cyclohexanamine egg hatching Biology (General) QH301-705.5
spellingShingle	nematode-trapping fungi <i>Duddingtonia flagrans</i> <i>Meloidogyne incognita</i> VOCs cyclohexanamine egg hatching Biology (General) QH301-705.5 Xiaoyu Mei Xin Wang Guohong Li Pathogenicity and Volatile Nematicidal Metabolites from <i>Duddingtonia flagrans</i> against <i>Meloidogyne incognita</i>
description	Plant parasitic nematodes, especially parasitic root-knot nematodes, are one of the most destructive plant pathogens worldwide. The control of plant root-knot nematodes is extremely challenging. <i>Duddingtonia flagrans</i> is a type of nematode-trapping fungi (NTF), which produces three-dimensional adhesive networks to trap nematodes. In this study, the pathogenicity and volatile organic compounds (VOCs) of the NTF <i>D. flagrans</i> against the plant root-knot nematode, <i>Meloidogyne incognita</i>, were investigated. The predatory process of <i>D. flagrans</i> trapping <i>M. incognita</i> was observed using scanning electron microscopy. Gas chromatography-mass spectrometry analysis of the VOCs from <i>D. flagrans</i> led to the identification of 52 metabolites, of which 11 main compounds were tested individually for their activity against <i>M. incognita</i>. Three compounds, cyclohexanamine, cyclohexanone, and cyclohexanol, were toxic to <i>M. incognita</i>. Furthermore, these three VOCs inhibited egg hatching of <i>M. incognita</i>. Cyclohexanamine showed the highest nematicidal activity, which can cause 97.93% mortality of <i>M. incognita</i> at 8.71 µM within 12 h. The number of hatched juveniles per egg mass after 3 days was just 8.44 when treated with 26.14 µM cyclohexanamine. This study is the first to demonstrate the nematicidal activity of VOCs produced by <i>D. flagrans</i> against <i>M. incognita</i>, which indicates that <i>D. flagrans</i> has the potential to biocontrol plant root-knot nematodes.
format	article
author	Xiaoyu Mei Xin Wang Guohong Li
author_facet	Xiaoyu Mei Xin Wang Guohong Li
author_sort	Xiaoyu Mei
title	Pathogenicity and Volatile Nematicidal Metabolites from <i>Duddingtonia flagrans</i> against <i>Meloidogyne incognita</i>
title_short	Pathogenicity and Volatile Nematicidal Metabolites from <i>Duddingtonia flagrans</i> against <i>Meloidogyne incognita</i>
title_full	Pathogenicity and Volatile Nematicidal Metabolites from <i>Duddingtonia flagrans</i> against <i>Meloidogyne incognita</i>
title_fullStr	Pathogenicity and Volatile Nematicidal Metabolites from <i>Duddingtonia flagrans</i> against <i>Meloidogyne incognita</i>
title_full_unstemmed	Pathogenicity and Volatile Nematicidal Metabolites from <i>Duddingtonia flagrans</i> against <i>Meloidogyne incognita</i>
title_sort	pathogenicity and volatile nematicidal metabolites from <i>duddingtonia flagrans</i> against <i>meloidogyne incognita</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/89eff4ea375d460d86738bc5a05b31f1
work_keys_str_mv	AT xiaoyumei pathogenicityandvolatilenematicidalmetabolitesfromiduddingtoniaflagransiagainstimeloidogyneincognitai AT xinwang pathogenicityandvolatilenematicidalmetabolitesfromiduddingtoniaflagransiagainstimeloidogyneincognitai AT guohongli pathogenicityandvolatilenematicidalmetabolitesfromiduddingtoniaflagransiagainstimeloidogyneincognitai
_version_	1718411208840708096

Pathogenicity and Volatile Nematicidal Metabolites from <i>Duddingtonia flagrans</i> against <i>Meloidogyne incognita</i>

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