Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1

Abstract Marek’s disease (MD), caused by Marek’s disease virus (MDV), is a lymphotropic neoplastic disease. Previous miRNAome analysis showed gga-miR-219b was significantly downregulated in MDV-induced lymphoma, and one of its potential target genes, B-cell chronic lymphocytic /lymphoma 11B (BCL11B)...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chunfang Zhao, Xin Li, Bo Han, Zhen You, Lujiang Qu, Changjun Liu, Jiuzhou Song, Ling Lian, Ning Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89f546ac085e442094a60b612f3db1c8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:89f546ac085e442094a60b612f3db1c8
record_format	dspace
spelling	oai:doaj.org-article:89f546ac085e442094a60b612f3db1c82021-12-02T16:06:06ZGga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB110.1038/s41598-017-04434-w2045-2322https://doaj.org/article/89f546ac085e442094a60b612f3db1c82017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04434-whttps://doaj.org/toc/2045-2322Abstract Marek’s disease (MD), caused by Marek’s disease virus (MDV), is a lymphotropic neoplastic disease. Previous miRNAome analysis showed gga-miR-219b was significantly downregulated in MDV-induced lymphoma, and one of its potential target genes, B-cell chronic lymphocytic /lymphoma 11B (BCL11B) was predicted. In this study, we further investigated the function of gga-miR-219b, and the gain/loss of function assay showed gga-miR-219b inhibited cell migration and reduced cell proliferation by promoting apoptosis not by cell cycle arrest. Gga-miR-219b also suppressed expression of two cell invasion-related genes MMP2 and MMP9. The results indicated suppressive effect of gga-miR-219b on MD tumorigenesis. The gene BCL11B was verified as a direct target gene of gga-miR-219b. RNA interference was performed to block BCL11B. As expected, the effects triggered by BCL11B downregulation were in accordance with that triggered by gga-miR-219b overexpression, suggesting that BCL11B was a stimulative regulator of MD transformation. Moreover, both gga-miR-219b and BCL11B influenced the expression of Meq gene, the most important oncogene in MDV. Additionally, gene expression level of anti-apoptotic genes BCL2 and BCL2L1 was downregulated and pro-apoptotic gene TNFSF10 was upregulated in MSB1 cells with gga-miR-219b overexpression or BCL11B knockdown, which suggested gga-miR-219b promoted cell apoptosis via regulating gene expression in the apoptosis pathways.Chunfang ZhaoXin LiBo HanZhen YouLujiang QuChangjun LiuJiuzhou SongLing LianNing YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chunfang Zhao Xin Li Bo Han Zhen You Lujiang Qu Changjun Liu Jiuzhou Song Ling Lian Ning Yang Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1
description	Abstract Marek’s disease (MD), caused by Marek’s disease virus (MDV), is a lymphotropic neoplastic disease. Previous miRNAome analysis showed gga-miR-219b was significantly downregulated in MDV-induced lymphoma, and one of its potential target genes, B-cell chronic lymphocytic /lymphoma 11B (BCL11B) was predicted. In this study, we further investigated the function of gga-miR-219b, and the gain/loss of function assay showed gga-miR-219b inhibited cell migration and reduced cell proliferation by promoting apoptosis not by cell cycle arrest. Gga-miR-219b also suppressed expression of two cell invasion-related genes MMP2 and MMP9. The results indicated suppressive effect of gga-miR-219b on MD tumorigenesis. The gene BCL11B was verified as a direct target gene of gga-miR-219b. RNA interference was performed to block BCL11B. As expected, the effects triggered by BCL11B downregulation were in accordance with that triggered by gga-miR-219b overexpression, suggesting that BCL11B was a stimulative regulator of MD transformation. Moreover, both gga-miR-219b and BCL11B influenced the expression of Meq gene, the most important oncogene in MDV. Additionally, gene expression level of anti-apoptotic genes BCL2 and BCL2L1 was downregulated and pro-apoptotic gene TNFSF10 was upregulated in MSB1 cells with gga-miR-219b overexpression or BCL11B knockdown, which suggested gga-miR-219b promoted cell apoptosis via regulating gene expression in the apoptosis pathways.
format	article
author	Chunfang Zhao Xin Li Bo Han Zhen You Lujiang Qu Changjun Liu Jiuzhou Song Ling Lian Ning Yang
author_facet	Chunfang Zhao Xin Li Bo Han Zhen You Lujiang Qu Changjun Liu Jiuzhou Song Ling Lian Ning Yang
author_sort	Chunfang Zhao
title	Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1
title_short	Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1
title_full	Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1
title_fullStr	Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1
title_full_unstemmed	Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1
title_sort	gga-mir-219b targeting bcl11b suppresses proliferation, migration and invasion of marek’s disease tumor cell msb1
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/89f546ac085e442094a60b612f3db1c8
work_keys_str_mv	AT chunfangzhao ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1 AT xinli ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1 AT bohan ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1 AT zhenyou ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1 AT lujiangqu ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1 AT changjunliu ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1 AT jiuzhousong ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1 AT linglian ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1 AT ningyang ggamir219btargetingbcl11bsuppressesproliferationmigrationandinvasionofmareksdiseasetumorcellmsb1
_version_	1718385099743952896

Gga-miR-219b targeting BCL11B suppresses proliferation, migration and invasion of Marek’s disease tumor cell MSB1

Ejemplares similares