Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species.
Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the fluorescence...
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Public Library of Science (PLoS)
2012
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oai:doaj.org-article:89f9e54d85784743aba5a44e237e12c92021-11-18T07:07:01ZUltraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species.1932-620310.1371/journal.pone.0044142https://doaj.org/article/89f9e54d85784743aba5a44e237e12c92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952902/?tool=EBIhttps://doaj.org/toc/1932-6203Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the fluorescence characteristics of bovine hemoglobin (BHb) in vitro. Data show that UVI results in fluorescence enhancement of BHb in a dose-dependent manner. Furthermore, UVI-induced fluorescence enhancement is significantly increased when BHb is pretreated with hydrogen peroxide (H(2)O(2)), a type of reactive oxygen species (ROS). Meanwhile, The water-soluble antioxidant vitamin C suppresses this UVI-induced fluorescence enhancement. In contrast, green light irradiation does not lead to fluorescence enhancement of BHb no matter whether H(2)O(2) is acting on the BHb solution or not. Taken together, these results indicate that catalysis of ROS and UVI-dependent irradiation play two key roles in the process of UVI-induced fluorescence enhancement of BHb.Leiting PanXiaoxu WangShuying YangXian WuImshik LeeXinzheng ZhangRomano A RuppJingjun XuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e44142 (2012) |
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Medicine R Science Q |
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Medicine R Science Q Leiting Pan Xiaoxu Wang Shuying Yang Xian Wu Imshik Lee Xinzheng Zhang Romano A Rupp Jingjun Xu Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species. |
| description |
Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the fluorescence characteristics of bovine hemoglobin (BHb) in vitro. Data show that UVI results in fluorescence enhancement of BHb in a dose-dependent manner. Furthermore, UVI-induced fluorescence enhancement is significantly increased when BHb is pretreated with hydrogen peroxide (H(2)O(2)), a type of reactive oxygen species (ROS). Meanwhile, The water-soluble antioxidant vitamin C suppresses this UVI-induced fluorescence enhancement. In contrast, green light irradiation does not lead to fluorescence enhancement of BHb no matter whether H(2)O(2) is acting on the BHb solution or not. Taken together, these results indicate that catalysis of ROS and UVI-dependent irradiation play two key roles in the process of UVI-induced fluorescence enhancement of BHb. |
| format |
article |
| author |
Leiting Pan Xiaoxu Wang Shuying Yang Xian Wu Imshik Lee Xinzheng Zhang Romano A Rupp Jingjun Xu |
| author_facet |
Leiting Pan Xiaoxu Wang Shuying Yang Xian Wu Imshik Lee Xinzheng Zhang Romano A Rupp Jingjun Xu |
| author_sort |
Leiting Pan |
| title |
Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species. |
| title_short |
Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species. |
| title_full |
Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species. |
| title_fullStr |
Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species. |
| title_full_unstemmed |
Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species. |
| title_sort |
ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/89f9e54d85784743aba5a44e237e12c9 |
| work_keys_str_mv |
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| _version_ |
1718423927130161152 |