Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil

Gustavo Milson Fabrício-Silva,1 Bruno Silva Poschetzky,2 Renata de Mello Perez,2 Ronaldo Carneiro dos Santos,2 Luciana Tricai Cavalini,3 Luís Cristóvão Porto1 1Histocompatibility and Cryopreservation Laboratory, 2Gastroenterology Service, Internal Medicine...

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Autores principales:	Fabrício-Silva GM, Poschetzky BS, Perez RM, Santos RC, Cavalini LT, Porto LC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/89fbfec49f0e49aa9bd1a74623af18a6
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spelling	oai:doaj.org-article:89fbfec49f0e49aa9bd1a74623af18a62021-12-02T06:20:22ZAssociation of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil1179-1535https://doaj.org/article/89fbfec49f0e49aa9bd1a74623af18a62015-11-01T00:00:00Zhttps://www.dovepress.com/association-of-cytokine-gene-polymorphisms-with-hepatitis-c-virus-infe-peer-reviewed-article-HMERhttps://doaj.org/toc/1179-1535Gustavo Milson Fabrício-Silva,1 Bruno Silva Poschetzky,2 Renata de Mello Perez,2 Ronaldo Carneiro dos Santos,2 Luciana Tricai Cavalini,3 Luís Cristóvão Porto1 1Histocompatibility and Cryopreservation Laboratory, 2Gastroenterology Service, Internal Medicine Department, 3Information Technology and Healthy Education Department, Rio de Janeiro State University, Rio de Janeiro, Brazil Background: The host immune response is an important indicator of the outcome of hepatitis C virus (HCV) infection and disease progression. The aim of this study was to explore cytokine gene polymorphisms as a candidate for susceptibility to persistent HCV infection or HCV spontaneous clearance in a population from Rio de Janeiro, Brazil. Methods: Genetic polymorphisms in the cytokines, tumor necrosis factor-alpha (–308), transforming growth factor-beta 1 (codons 10 and 25), interleukin-10 (IL-10; –1082 and –592), IL-6 (–174), and interferon-gamma (+874) were analyzed by polymerase chain reaction sequence-specific primers in 245 patients with chronic hepatitis C (CHC), 41 spontaneous recovery (SR) patients, and 189 healthy volunteers. Further, polymorphisms in IL-28B (rs12979860, rs12980275, and rs8099917) were assessed by real-time polymerase chain reaction in all groups. Results: The IL-28B rs12979860 CC and rs12980275 AA genotypes were significantly associated with SR of HCV infection and response to therapy, whereas the C allele of IL-6 (–174) was associated with protection to CHC. A multivariate analysis showed that the male sex and IL-28B rs12979860 CT and TT and transforming growth factor-beta 1 (codon 10) TC genotypes were factors associated with CHC. Additionally, IL-6 (–174) C allele was increased in SR patients compared with patients with CHC. Conclusion: IL-28B polymorphisms are associated with spontaneous clearance of HCV and response to therapy in a Brazilian population. Also, IL-6 (–174) C allele is involved in SR and decreased inflammation scores. Keywords: interleukin-28, interleukin-6, SNP, inflammation, HCV clearanceFabrício-Silva GMPoschetzky BSPerez RMSantos RCCavalini LTPorto LCDove Medical PressarticleDiseases of the digestive system. GastroenterologyRC799-869ENHepatic Medicine: Evidence and Research, Vol 2015, Iss default, Pp 71-79 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	Diseases of the digestive system. Gastroenterology RC799-869 Fabrício-Silva GM Poschetzky BS Perez RM Santos RC Cavalini LT Porto LC Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil
description	Gustavo Milson Fabrício-Silva,1 Bruno Silva Poschetzky,2 Renata de Mello Perez,2 Ronaldo Carneiro dos Santos,2 Luciana Tricai Cavalini,3 Luís Cristóvão Porto1 1Histocompatibility and Cryopreservation Laboratory, 2Gastroenterology Service, Internal Medicine Department, 3Information Technology and Healthy Education Department, Rio de Janeiro State University, Rio de Janeiro, Brazil Background: The host immune response is an important indicator of the outcome of hepatitis C virus (HCV) infection and disease progression. The aim of this study was to explore cytokine gene polymorphisms as a candidate for susceptibility to persistent HCV infection or HCV spontaneous clearance in a population from Rio de Janeiro, Brazil. Methods: Genetic polymorphisms in the cytokines, tumor necrosis factor-alpha (–308), transforming growth factor-beta 1 (codons 10 and 25), interleukin-10 (IL-10; –1082 and –592), IL-6 (–174), and interferon-gamma (+874) were analyzed by polymerase chain reaction sequence-specific primers in 245 patients with chronic hepatitis C (CHC), 41 spontaneous recovery (SR) patients, and 189 healthy volunteers. Further, polymorphisms in IL-28B (rs12979860, rs12980275, and rs8099917) were assessed by real-time polymerase chain reaction in all groups. Results: The IL-28B rs12979860 CC and rs12980275 AA genotypes were significantly associated with SR of HCV infection and response to therapy, whereas the C allele of IL-6 (–174) was associated with protection to CHC. A multivariate analysis showed that the male sex and IL-28B rs12979860 CT and TT and transforming growth factor-beta 1 (codon 10) TC genotypes were factors associated with CHC. Additionally, IL-6 (–174) C allele was increased in SR patients compared with patients with CHC. Conclusion: IL-28B polymorphisms are associated with spontaneous clearance of HCV and response to therapy in a Brazilian population. Also, IL-6 (–174) C allele is involved in SR and decreased inflammation scores. Keywords: interleukin-28, interleukin-6, SNP, inflammation, HCV clearance
format	article
author	Fabrício-Silva GM Poschetzky BS Perez RM Santos RC Cavalini LT Porto LC
author_facet	Fabrício-Silva GM Poschetzky BS Perez RM Santos RC Cavalini LT Porto LC
author_sort	Fabrício-Silva GM
title	Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil
title_short	Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil
title_full	Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil
title_fullStr	Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil
title_full_unstemmed	Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil
title_sort	association of cytokine gene polymorphisms with hepatitis c virus infection in a population from rio de janeiro, brazil
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/89fbfec49f0e49aa9bd1a74623af18a6
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Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil

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