Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.

Epithelial ovarian cancer is a highly heterogeneous disease and remains the most lethal gynaecological malignancy in the Western world. Therapeutic approaches need to account for inter-patient and intra-tumoural heterogeneity and detailed characterization of in vitro models representing the differen...

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Autores principales:	Corine M Beaufort, Jean C A Helmijr, Anna M Piskorz, Marlous Hoogstraat, Kirsten Ruigrok-Ritstier, Nicolle Besselink, Muhammed Murtaza, Wilfred F J van Ĳcken, Anouk A J Heine, Marcel Smid, Marco J Koudijs, James D Brenton, Els M J J Berns, Jozien Helleman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/89fef8c147544e4ca580af119453cff1
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spelling	oai:doaj.org-article:89fef8c147544e4ca580af119453cff12021-11-25T06:00:15ZOvarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.1932-620310.1371/journal.pone.0103988https://doaj.org/article/89fef8c147544e4ca580af119453cff12014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0103988https://doaj.org/toc/1932-6203Epithelial ovarian cancer is a highly heterogeneous disease and remains the most lethal gynaecological malignancy in the Western world. Therapeutic approaches need to account for inter-patient and intra-tumoural heterogeneity and detailed characterization of in vitro models representing the different histological and molecular ovarian cancer subtypes is critical to enable reliable preclinical testing. There are approximately 100 publicly available ovarian cancer cell lines but their cellular and molecular characteristics are largely undescribed. We have characterized 39 ovarian cancer cell lines under uniform conditions for growth characteristics, mRNA/microRNA expression, exon sequencing, drug response for clinically-relevant therapeutics and collated all available information on the original clinical features and site of origin. We tested for statistical associations between the cellular and molecular features of the lines and clinical features. Of the 39 ovarian cancer cell lines, 14 were assigned as high-grade serous, four serous-type, one low-grade serous and 20 non-serous type. Three morphological subtypes: Epithelial (n = 21), Round (n = 7) and Spindle (n = 12) were identified that showed distinct biological and molecular characteristics, including overexpression of cell movement and migration-associated genes in the Spindle subtype. Comparison with the original clinical data showed association of the spindle-like tumours with metastasis, advanced stage, suboptimal debulking and poor prognosis. In addition, the expression profiles of Spindle, Round and Epithelial morphologies clustered with the previously described C1-stromal, C5-mesenchymal and C4 ovarian subtype expression profiles respectively. Comprehensive profiling of 39 ovarian cancer cell lines under controlled, uniform conditions demonstrates clinically relevant cellular and genomic characteristics. This data provides a rational basis for selecting models to develop specific treatment approaches for histological and molecular subtypes of ovarian cancer.Corine M BeaufortJean C A HelmijrAnna M PiskorzMarlous HoogstraatKirsten Ruigrok-RitstierNicolle BesselinkMuhammed MurtazaWilfred F J van ĲckenAnouk A J HeineMarcel SmidMarco J KoudijsJames D BrentonEls M J J BernsJozien HellemanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e103988 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Corine M Beaufort Jean C A Helmijr Anna M Piskorz Marlous Hoogstraat Kirsten Ruigrok-Ritstier Nicolle Besselink Muhammed Murtaza Wilfred F J van Ĳcken Anouk A J Heine Marcel Smid Marco J Koudijs James D Brenton Els M J J Berns Jozien Helleman Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
description	Epithelial ovarian cancer is a highly heterogeneous disease and remains the most lethal gynaecological malignancy in the Western world. Therapeutic approaches need to account for inter-patient and intra-tumoural heterogeneity and detailed characterization of in vitro models representing the different histological and molecular ovarian cancer subtypes is critical to enable reliable preclinical testing. There are approximately 100 publicly available ovarian cancer cell lines but their cellular and molecular characteristics are largely undescribed. We have characterized 39 ovarian cancer cell lines under uniform conditions for growth characteristics, mRNA/microRNA expression, exon sequencing, drug response for clinically-relevant therapeutics and collated all available information on the original clinical features and site of origin. We tested for statistical associations between the cellular and molecular features of the lines and clinical features. Of the 39 ovarian cancer cell lines, 14 were assigned as high-grade serous, four serous-type, one low-grade serous and 20 non-serous type. Three morphological subtypes: Epithelial (n = 21), Round (n = 7) and Spindle (n = 12) were identified that showed distinct biological and molecular characteristics, including overexpression of cell movement and migration-associated genes in the Spindle subtype. Comparison with the original clinical data showed association of the spindle-like tumours with metastasis, advanced stage, suboptimal debulking and poor prognosis. In addition, the expression profiles of Spindle, Round and Epithelial morphologies clustered with the previously described C1-stromal, C5-mesenchymal and C4 ovarian subtype expression profiles respectively. Comprehensive profiling of 39 ovarian cancer cell lines under controlled, uniform conditions demonstrates clinically relevant cellular and genomic characteristics. This data provides a rational basis for selecting models to develop specific treatment approaches for histological and molecular subtypes of ovarian cancer.
format	article
author	Corine M Beaufort Jean C A Helmijr Anna M Piskorz Marlous Hoogstraat Kirsten Ruigrok-Ritstier Nicolle Besselink Muhammed Murtaza Wilfred F J van Ĳcken Anouk A J Heine Marcel Smid Marco J Koudijs James D Brenton Els M J J Berns Jozien Helleman
author_facet	Corine M Beaufort Jean C A Helmijr Anna M Piskorz Marlous Hoogstraat Kirsten Ruigrok-Ritstier Nicolle Besselink Muhammed Murtaza Wilfred F J van Ĳcken Anouk A J Heine Marcel Smid Marco J Koudijs James D Brenton Els M J J Berns Jozien Helleman
author_sort	Corine M Beaufort
title	Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
title_short	Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
title_full	Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
title_fullStr	Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
title_full_unstemmed	Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
title_sort	ovarian cancer cell line panel (occp): clinical importance of in vitro morphological subtypes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/89fef8c147544e4ca580af119453cff1
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Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.

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