Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment

Abstract As a medicinal plant widely planted in southwest karst of China, the study of adaptation mechanisms of Lonicera confusa, especially to karst calcium-rich environment, can provide important theoretical basis for repairing desertification by genetic engineering. In this study, the Ca2+ imagin...

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Autores principales:	Wenwen Jin, Yan Long, Chunhua Fu, Libin Zhang, Jun Xiang, Baoshan Wang, Maoteng Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a093bde420b42168d7d46d0114bfb0d
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spelling	oai:doaj.org-article:8a093bde420b42168d7d46d0114bfb0d2021-12-02T16:08:13ZCa2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment10.1038/s41598-018-25611-52045-2322https://doaj.org/article/8a093bde420b42168d7d46d0114bfb0d2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25611-5https://doaj.org/toc/2045-2322Abstract As a medicinal plant widely planted in southwest karst of China, the study of adaptation mechanisms of Lonicera confusa, especially to karst calcium-rich environment, can provide important theoretical basis for repairing desertification by genetic engineering. In this study, the Ca2+ imaging in the leaves of L. confusa was explored by LSCM (Laser Scanning Confocal Microscopy) and TEM (Transmission Electron Microscopy), which revealed that the calcium could be transported to gland, epidermal hair and stoma in the leaves of L. confusa in high-Ca2+ environment. In addition, we simulated the growth environment of L. confusa and identified DEGs (Differentially Expressed Genes) under different Ca2+ concentrations by RNA sequencing. Further analysis showed that these DEGs were assigned with some important biological processes. Furthermore, a complex protein-protein interaction network among DEGs in L. Confusa was constructed and some important regulatory genes and transcription factors were identified. Taken together, this study displayed the Ca2+ transport and the accumulation of Ca2+ channels and pools in L. Confusa with high-Ca2+ treatment. Moreover, RNA sequencing provided a global picture of differential gene expression patterns in L. Confusa with high-Ca2+ treatment, which will help to reveal the molecular mechanism of the adaptation of L. confusa to high-Ca2+ environment in the future.Wenwen JinYan LongChunhua FuLibin ZhangJun XiangBaoshan WangMaoteng LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenwen Jin Yan Long Chunhua Fu Libin Zhang Jun Xiang Baoshan Wang Maoteng Li Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment
description	Abstract As a medicinal plant widely planted in southwest karst of China, the study of adaptation mechanisms of Lonicera confusa, especially to karst calcium-rich environment, can provide important theoretical basis for repairing desertification by genetic engineering. In this study, the Ca2+ imaging in the leaves of L. confusa was explored by LSCM (Laser Scanning Confocal Microscopy) and TEM (Transmission Electron Microscopy), which revealed that the calcium could be transported to gland, epidermal hair and stoma in the leaves of L. confusa in high-Ca2+ environment. In addition, we simulated the growth environment of L. confusa and identified DEGs (Differentially Expressed Genes) under different Ca2+ concentrations by RNA sequencing. Further analysis showed that these DEGs were assigned with some important biological processes. Furthermore, a complex protein-protein interaction network among DEGs in L. Confusa was constructed and some important regulatory genes and transcription factors were identified. Taken together, this study displayed the Ca2+ transport and the accumulation of Ca2+ channels and pools in L. Confusa with high-Ca2+ treatment. Moreover, RNA sequencing provided a global picture of differential gene expression patterns in L. Confusa with high-Ca2+ treatment, which will help to reveal the molecular mechanism of the adaptation of L. confusa to high-Ca2+ environment in the future.
format	article
author	Wenwen Jin Yan Long Chunhua Fu Libin Zhang Jun Xiang Baoshan Wang Maoteng Li
author_facet	Wenwen Jin Yan Long Chunhua Fu Libin Zhang Jun Xiang Baoshan Wang Maoteng Li
author_sort	Wenwen Jin
title	Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment
title_short	Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment
title_full	Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment
title_fullStr	Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment
title_full_unstemmed	Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment
title_sort	ca2+ imaging and gene expression profiling of lonicera confusa in response to calcium-rich environment
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8a093bde420b42168d7d46d0114bfb0d
work_keys_str_mv	AT wenwenjin ca2imagingandgeneexpressionprofilingofloniceraconfusainresponsetocalciumrichenvironment AT yanlong ca2imagingandgeneexpressionprofilingofloniceraconfusainresponsetocalciumrichenvironment AT chunhuafu ca2imagingandgeneexpressionprofilingofloniceraconfusainresponsetocalciumrichenvironment AT libinzhang ca2imagingandgeneexpressionprofilingofloniceraconfusainresponsetocalciumrichenvironment AT junxiang ca2imagingandgeneexpressionprofilingofloniceraconfusainresponsetocalciumrichenvironment AT baoshanwang ca2imagingandgeneexpressionprofilingofloniceraconfusainresponsetocalciumrichenvironment AT maotengli ca2imagingandgeneexpressionprofilingofloniceraconfusainresponsetocalciumrichenvironment
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Ca2+ imaging and gene expression profiling of Lonicera Confusa in response to calcium-rich environment

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