Ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model

Abstract Emerging evidence has highlighted the connection between exposure to air pollution and the increased risk of obesity, metabolic syndrome, and comorbidities. Given the recent interest in studying the effects of ultrafine particle (UFP) on the health of obese individuals, this study examined...

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Autores principales: Kundi Yang, Mengyang Xu, Jingyi Cao, Qi Zhu, Monica Rahman, Britt A. Holmén, Naomi K. Fukagawa, Jiangjiang Zhu
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:8a09d933febe4ff79febb6c240bf8caa2021-12-02T11:45:02ZUltrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model10.1038/s41598-021-85784-42045-2322https://doaj.org/article/8a09d933febe4ff79febb6c240bf8caa2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85784-4https://doaj.org/toc/2045-2322Abstract Emerging evidence has highlighted the connection between exposure to air pollution and the increased risk of obesity, metabolic syndrome, and comorbidities. Given the recent interest in studying the effects of ultrafine particle (UFP) on the health of obese individuals, this study examined the effects of gastrointestinal UFP exposure on gut microbial composition and metabolic function using an in vivo murine model of obesity in both sexes. UFPs generated from light-duty diesel engine combustion of petrodiesel (B0) and a petrodiesel/biodiesel fuel blend (80:20 v/v, B20) were administered orally. Multi-omics approaches, including liquid chromatography–mass spectrometry (LC–MS) based targeted metabolomics and 16S rRNA gene sequence analysis, semi-quantitatively compared the effects of 10-day UFP exposures on obese C57B6 mouse gut microbial population, changes in diversity and community function compared to a phosphate buffer solution (PBS) control group. Our results show that sex-specific differences in the gut microbial population in response to UFP exposure can be observed, as UFPs appear to have a differential impact on several bacterial families in males and females. Meanwhile, the alteration of seventy-five metabolites from the gut microbial metabolome varied significantly (ANOVA p < 0.05) across the PBS control, B0, and B20 groups. Multivariate analyses revealed that the fuel-type specific disruption to the microbial metabolome was observed in both sexes, with stronger disruptive effects found in females in comparison to male obese mice. Metabolic signatures of bacterial cellular oxidative stress, such as the decreased concentration of nucleotides and lipids and increased concentrations of carbohydrate, energy, and vitamin metabolites were detected. Furthermore, blood metabolites from the obese mice were differentially affected by the fuel types used to generate the UFPs (B0 vs. B20).Kundi YangMengyang XuJingyi CaoQi ZhuMonica RahmanBritt A. HolménNaomi K. FukagawaJiangjiang ZhuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Kundi Yang
Mengyang Xu
Jingyi Cao
Qi Zhu
Monica Rahman
Britt A. Holmén
Naomi K. Fukagawa
Jiangjiang Zhu
Ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model
description Abstract Emerging evidence has highlighted the connection between exposure to air pollution and the increased risk of obesity, metabolic syndrome, and comorbidities. Given the recent interest in studying the effects of ultrafine particle (UFP) on the health of obese individuals, this study examined the effects of gastrointestinal UFP exposure on gut microbial composition and metabolic function using an in vivo murine model of obesity in both sexes. UFPs generated from light-duty diesel engine combustion of petrodiesel (B0) and a petrodiesel/biodiesel fuel blend (80:20 v/v, B20) were administered orally. Multi-omics approaches, including liquid chromatography–mass spectrometry (LC–MS) based targeted metabolomics and 16S rRNA gene sequence analysis, semi-quantitatively compared the effects of 10-day UFP exposures on obese C57B6 mouse gut microbial population, changes in diversity and community function compared to a phosphate buffer solution (PBS) control group. Our results show that sex-specific differences in the gut microbial population in response to UFP exposure can be observed, as UFPs appear to have a differential impact on several bacterial families in males and females. Meanwhile, the alteration of seventy-five metabolites from the gut microbial metabolome varied significantly (ANOVA p < 0.05) across the PBS control, B0, and B20 groups. Multivariate analyses revealed that the fuel-type specific disruption to the microbial metabolome was observed in both sexes, with stronger disruptive effects found in females in comparison to male obese mice. Metabolic signatures of bacterial cellular oxidative stress, such as the decreased concentration of nucleotides and lipids and increased concentrations of carbohydrate, energy, and vitamin metabolites were detected. Furthermore, blood metabolites from the obese mice were differentially affected by the fuel types used to generate the UFPs (B0 vs. B20).
format article
author Kundi Yang
Mengyang Xu
Jingyi Cao
Qi Zhu
Monica Rahman
Britt A. Holmén
Naomi K. Fukagawa
Jiangjiang Zhu
author_facet Kundi Yang
Mengyang Xu
Jingyi Cao
Qi Zhu
Monica Rahman
Britt A. Holmén
Naomi K. Fukagawa
Jiangjiang Zhu
author_sort Kundi Yang
title Ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model
title_short Ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model
title_full Ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model
title_fullStr Ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model
title_full_unstemmed Ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model
title_sort ultrafine particles altered gut microbial population and metabolic profiles in a sex-specific manner in an obese mouse model
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/8a09d933febe4ff79febb6c240bf8caa
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