Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe

Abstract Neutral DNA analogs as probes for the detection of target oligomers on the biosensors based on the field-effect transistor (FET) configuration feature advantages in the enhancement of sensitivity and signal-to-noise ratio. Herein, we used phosphate-methylated nucleotides to synthesize two p...

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Autores principales:	Wen-Pin Hu, Chih-Chin Tsai, Yuh-Shyong Yang, Hardy Wai-Hong Chan, Wen-Yih Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a0c489ed7ad487bb2c0347390cea90e
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spelling	oai:doaj.org-article:8a0c489ed7ad487bb2c0347390cea90e2021-12-02T15:09:10ZSynergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe10.1038/s41598-018-30996-42045-2322https://doaj.org/article/8a0c489ed7ad487bb2c0347390cea90e2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30996-4https://doaj.org/toc/2045-2322Abstract Neutral DNA analogs as probes for the detection of target oligomers on the biosensors based on the field-effect transistor (FET) configuration feature advantages in the enhancement of sensitivity and signal-to-noise ratio. Herein, we used phosphate-methylated nucleotides to synthesize two partially neutralized chimeric DNA products and a fully neutralized DNA sequence and adopted a regular DNA oligomer as probes on the polycrystalline silicon nanowire (NW) FET devices. The sequences of two neutralized chimeric DNAs close to the 5′ end were alternately modified with the phosphate-methylated nucleotides, and all probes were immobilized via their 5′ end on the NW surface. The non-specific-to-specific binding ratio indicated that the two 5′-end partially neutralized chimeric DNAs featured better performance than the regular and fully neutralized DNA oligomers. The partially neutralized probe design reduces the ionic strength needed for hybridization and increases the Debye length of detection, thus promoting the detection sensitivity of FET and achieving the limit of detection of 0.1 fM. By using an appropriate probe design, applying DNA oligomers with embedded phosphate-methylated nucleotides in the FET biosensors is a promising way for gene detection with high sensitivity and specificity.Wen-Pin HuChih-Chin TsaiYuh-Shyong YangHardy Wai-Hong ChanWen-Yih ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wen-Pin Hu Chih-Chin Tsai Yuh-Shyong Yang Hardy Wai-Hong Chan Wen-Yih Chen Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe
description	Abstract Neutral DNA analogs as probes for the detection of target oligomers on the biosensors based on the field-effect transistor (FET) configuration feature advantages in the enhancement of sensitivity and signal-to-noise ratio. Herein, we used phosphate-methylated nucleotides to synthesize two partially neutralized chimeric DNA products and a fully neutralized DNA sequence and adopted a regular DNA oligomer as probes on the polycrystalline silicon nanowire (NW) FET devices. The sequences of two neutralized chimeric DNAs close to the 5′ end were alternately modified with the phosphate-methylated nucleotides, and all probes were immobilized via their 5′ end on the NW surface. The non-specific-to-specific binding ratio indicated that the two 5′-end partially neutralized chimeric DNAs featured better performance than the regular and fully neutralized DNA oligomers. The partially neutralized probe design reduces the ionic strength needed for hybridization and increases the Debye length of detection, thus promoting the detection sensitivity of FET and achieving the limit of detection of 0.1 fM. By using an appropriate probe design, applying DNA oligomers with embedded phosphate-methylated nucleotides in the FET biosensors is a promising way for gene detection with high sensitivity and specificity.
format	article
author	Wen-Pin Hu Chih-Chin Tsai Yuh-Shyong Yang Hardy Wai-Hong Chan Wen-Yih Chen
author_facet	Wen-Pin Hu Chih-Chin Tsai Yuh-Shyong Yang Hardy Wai-Hong Chan Wen-Yih Chen
author_sort	Wen-Pin Hu
title	Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe
title_short	Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe
title_full	Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe
title_fullStr	Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe
title_full_unstemmed	Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe
title_sort	synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized dna as probe
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8a0c489ed7ad487bb2c0347390cea90e
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Synergetic improvements of sensitivity and specificity of nanowire field effect transistor gene chip by designing neutralized DNA as probe

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