The Response of Mitochondrial Respiration and Quantity in Skeletal Muscle and Adipose Tissue to Exercise in Humans with Prediabetes
Background: Mitochondrial dysfunction has been implicated in the pathogenesis of type 2 diabetes, but its contribution to the early stages of dysglycemia remains poorly understood. By collecting a high-resolution stage-based spectrum of dysglycemia, our study fills this gap by evaluating derangement...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/8a13f5dbe8c9450ead54961843855293 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:8a13f5dbe8c9450ead54961843855293 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:8a13f5dbe8c9450ead549618438552932021-11-25T17:10:24ZThe Response of Mitochondrial Respiration and Quantity in Skeletal Muscle and Adipose Tissue to Exercise in Humans with Prediabetes10.3390/cells101130132073-4409https://doaj.org/article/8a13f5dbe8c9450ead549618438552932021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3013https://doaj.org/toc/2073-4409Background: Mitochondrial dysfunction has been implicated in the pathogenesis of type 2 diabetes, but its contribution to the early stages of dysglycemia remains poorly understood. By collecting a high-resolution stage-based spectrum of dysglycemia, our study fills this gap by evaluating derangement in both the function and quantity of mitochondria. We sampled mitochondria in skeletal muscle and subcutaneous adipose tissues of subjects with progressive advancement of dysglycemia under a three-month exercise intervention. Methods: We measured clinical metabolic parameters and gathered skeletal muscle and adipose tissue biopsies before and after the three-month exercise intervention. We then assayed the number of mitochondria via citrate synthase (CS) activity and functional parameters with high-resolution respirometry. Results: In muscle, there were no differences in mitochondrial quantity or function at baseline between normoglycemics and prediabetics. However, the intervention caused improvement in CS activity, implying an increase in mitochondrial quantity. By contrast in adipose tissue, baseline differences in CS activity were present, with the lowest CS activity coincident with impaired fasting glucose and impaired glucose tolerance (IFG + IGT). Finally, CS activity, but few of the functional metrics, improved under the intervention. Conclusions: We show that in prediabetes, no differences in the function or amount of mitochondria (measured by CS activity) in skeletal muscle are apparent, but in adipose tissue of subjects with IFG + IGT, a significantly reduced activity of CS was observed. Finally, metabolic improvements under the exercise correlate to improvements in the amount, rather than function, of mitochondria in both tissues.Lukasz SzczerbinskiMark Alan TaylorUrszula PuchtaPaulina KonopkaAdam PaszkoAnna CitkoKarol SzczerbinskiJoanna GoscikMaria GorskaSteen LarsenAdam KretowskiMDPI AGarticletype 2 diabetesobesitymitochondriamuscleadipose tissuefatBiology (General)QH301-705.5ENCells, Vol 10, Iss 3013, p 3013 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
type 2 diabetes obesity mitochondria muscle adipose tissue fat Biology (General) QH301-705.5 |
| spellingShingle |
type 2 diabetes obesity mitochondria muscle adipose tissue fat Biology (General) QH301-705.5 Lukasz Szczerbinski Mark Alan Taylor Urszula Puchta Paulina Konopka Adam Paszko Anna Citko Karol Szczerbinski Joanna Goscik Maria Gorska Steen Larsen Adam Kretowski The Response of Mitochondrial Respiration and Quantity in Skeletal Muscle and Adipose Tissue to Exercise in Humans with Prediabetes |
| description |
Background: Mitochondrial dysfunction has been implicated in the pathogenesis of type 2 diabetes, but its contribution to the early stages of dysglycemia remains poorly understood. By collecting a high-resolution stage-based spectrum of dysglycemia, our study fills this gap by evaluating derangement in both the function and quantity of mitochondria. We sampled mitochondria in skeletal muscle and subcutaneous adipose tissues of subjects with progressive advancement of dysglycemia under a three-month exercise intervention. Methods: We measured clinical metabolic parameters and gathered skeletal muscle and adipose tissue biopsies before and after the three-month exercise intervention. We then assayed the number of mitochondria via citrate synthase (CS) activity and functional parameters with high-resolution respirometry. Results: In muscle, there were no differences in mitochondrial quantity or function at baseline between normoglycemics and prediabetics. However, the intervention caused improvement in CS activity, implying an increase in mitochondrial quantity. By contrast in adipose tissue, baseline differences in CS activity were present, with the lowest CS activity coincident with impaired fasting glucose and impaired glucose tolerance (IFG + IGT). Finally, CS activity, but few of the functional metrics, improved under the intervention. Conclusions: We show that in prediabetes, no differences in the function or amount of mitochondria (measured by CS activity) in skeletal muscle are apparent, but in adipose tissue of subjects with IFG + IGT, a significantly reduced activity of CS was observed. Finally, metabolic improvements under the exercise correlate to improvements in the amount, rather than function, of mitochondria in both tissues. |
| format |
article |
| author |
Lukasz Szczerbinski Mark Alan Taylor Urszula Puchta Paulina Konopka Adam Paszko Anna Citko Karol Szczerbinski Joanna Goscik Maria Gorska Steen Larsen Adam Kretowski |
| author_facet |
Lukasz Szczerbinski Mark Alan Taylor Urszula Puchta Paulina Konopka Adam Paszko Anna Citko Karol Szczerbinski Joanna Goscik Maria Gorska Steen Larsen Adam Kretowski |
| author_sort |
Lukasz Szczerbinski |
| title |
The Response of Mitochondrial Respiration and Quantity in Skeletal Muscle and Adipose Tissue to Exercise in Humans with Prediabetes |
| title_short |
The Response of Mitochondrial Respiration and Quantity in Skeletal Muscle and Adipose Tissue to Exercise in Humans with Prediabetes |
| title_full |
The Response of Mitochondrial Respiration and Quantity in Skeletal Muscle and Adipose Tissue to Exercise in Humans with Prediabetes |
| title_fullStr |
The Response of Mitochondrial Respiration and Quantity in Skeletal Muscle and Adipose Tissue to Exercise in Humans with Prediabetes |
| title_full_unstemmed |
The Response of Mitochondrial Respiration and Quantity in Skeletal Muscle and Adipose Tissue to Exercise in Humans with Prediabetes |
| title_sort |
response of mitochondrial respiration and quantity in skeletal muscle and adipose tissue to exercise in humans with prediabetes |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/8a13f5dbe8c9450ead54961843855293 |
| work_keys_str_mv |
AT lukaszszczerbinski theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT markalantaylor theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT urszulapuchta theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT paulinakonopka theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT adampaszko theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT annacitko theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT karolszczerbinski theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT joannagoscik theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT mariagorska theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT steenlarsen theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT adamkretowski theresponseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT lukaszszczerbinski responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT markalantaylor responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT urszulapuchta responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT paulinakonopka responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT adampaszko responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT annacitko responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT karolszczerbinski responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT joannagoscik responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT mariagorska responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT steenlarsen responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes AT adamkretowski responseofmitochondrialrespirationandquantityinskeletalmuscleandadiposetissuetoexerciseinhumanswithprediabetes |
| _version_ |
1718412662089449472 |