Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis

Paulina Mazur,1 Iwona Skiba-Kurek,2 Paulina Mrowiec,2 Elżbieta Karczewska,2 Ryszard Drożdż1 1Department of Medical Diagnostics, Faculty of Pharmacy, Jagiellonian University Collegium Medicum, Cracow, Poland; 2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Col...

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Autores principales:	Mazur P, Skiba-Kurek I, Mrowiec P, Karczewska E, Drożdż R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	gentamicin silver nanoparticles reactive oxygen species multidrug-resistant staphylococcus epidermidis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8a191da83dbf450e8fe9b99f332dbd21
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spelling	oai:doaj.org-article:8a191da83dbf450e8fe9b99f332dbd212021-12-02T10:04:44ZSynergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis1178-2013https://doaj.org/article/8a191da83dbf450e8fe9b99f332dbd212020-05-01T00:00:00Zhttps://www.dovepress.com/synergistic-ros-associated-antimicrobial-activity-of-silver-nanopartic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Paulina Mazur,1 Iwona Skiba-Kurek,2 Paulina Mrowiec,2 Elżbieta Karczewska,2 Ryszard Drożdż1 1Department of Medical Diagnostics, Faculty of Pharmacy, Jagiellonian University Collegium Medicum, Cracow, Poland; 2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Collegium Medicum, Cracow, PolandCorrespondence: Ryszard DrożdżFaculty of Pharmacy, Department of Medical Diagnostics, Jagiellonian University, Cracow, PolandTel/Fax +48 12 6205985Email ryszard.drozdz@uj.edu.plIntroduction: Increasing bacteria resistance to antibiotics is a major problem of healthcare system. There is a need for solutions that broaden the spectrum of bactericidal agents improving the efficacy of commonly used antibiotics. One of the promising directions of search are silver nanoparticles (obtained by different methods and displaying diversified physical and chemical properties), and their combination with antibiotics.Purpose: In this study, we tested the role of reactive oxygen species in the mechanism of synergistic antibacterial activity of gentamicin and Tween-stabilized silver nanoparticles against gentamicin-resistant clinical strains of Staphylococcus epidermidis.Methods: Synergistic bactericidal activity of gentamicin and silver nanoparticles stabilized with non-ionic detergent (Tween 80) was tested by the checkerboard titration method on microtiter plates. Detection of reactive oxygen species was based on the chemiluminescence of luminol.Results: Hydrophilic non-ionic surface functionalization of silver nanoparticles enabled the existence of non-aggregated active nanoparticles in a complex bacterial culture medium. Tween-stabilized silver nanoparticles in combination with gentamicin exhibited bactericidal activity against multidrug-resistant biofilm forming clinical strains of Staphylococcus epidermidis. A synergistic effect significantly decreased the minimal inhibitory concentration of gentamicin (the antibiotic with numerous undesirable effects). Gentamicin significantly enhanced the generation of reactive oxygen species by silver nanoparticles.Conclusion: Generation of reactive oxygen species by Tween-coated metallic silver nanoparticles was significantly enhanced by gentamicin, confirming the hypothesis of oxidative-associated mechanism of the synergistic antibacterial effect of the gentamicin-silver nanoparticles complex.Keywords: gentamicin, silver nanoparticles, reactive oxygen species, multidrug-resistant, Staphylococcus epidermidisMazur PSkiba-Kurek IMrowiec PKarczewska EDrożdż RDove Medical Pressarticlegentamicinsilver nanoparticlesreactive oxygen speciesmultidrug-resistantstaphylococcus epidermidisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3551-3562 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	gentamicin silver nanoparticles reactive oxygen species multidrug-resistant staphylococcus epidermidis Medicine (General) R5-920
spellingShingle	gentamicin silver nanoparticles reactive oxygen species multidrug-resistant staphylococcus epidermidis Medicine (General) R5-920 Mazur P Skiba-Kurek I Mrowiec P Karczewska E Drożdż R Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis
description	Paulina Mazur,1 Iwona Skiba-Kurek,2 Paulina Mrowiec,2 Elżbieta Karczewska,2 Ryszard Drożdż1 1Department of Medical Diagnostics, Faculty of Pharmacy, Jagiellonian University Collegium Medicum, Cracow, Poland; 2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Collegium Medicum, Cracow, PolandCorrespondence: Ryszard DrożdżFaculty of Pharmacy, Department of Medical Diagnostics, Jagiellonian University, Cracow, PolandTel/Fax +48 12 6205985Email ryszard.drozdz@uj.edu.plIntroduction: Increasing bacteria resistance to antibiotics is a major problem of healthcare system. There is a need for solutions that broaden the spectrum of bactericidal agents improving the efficacy of commonly used antibiotics. One of the promising directions of search are silver nanoparticles (obtained by different methods and displaying diversified physical and chemical properties), and their combination with antibiotics.Purpose: In this study, we tested the role of reactive oxygen species in the mechanism of synergistic antibacterial activity of gentamicin and Tween-stabilized silver nanoparticles against gentamicin-resistant clinical strains of Staphylococcus epidermidis.Methods: Synergistic bactericidal activity of gentamicin and silver nanoparticles stabilized with non-ionic detergent (Tween 80) was tested by the checkerboard titration method on microtiter plates. Detection of reactive oxygen species was based on the chemiluminescence of luminol.Results: Hydrophilic non-ionic surface functionalization of silver nanoparticles enabled the existence of non-aggregated active nanoparticles in a complex bacterial culture medium. Tween-stabilized silver nanoparticles in combination with gentamicin exhibited bactericidal activity against multidrug-resistant biofilm forming clinical strains of Staphylococcus epidermidis. A synergistic effect significantly decreased the minimal inhibitory concentration of gentamicin (the antibiotic with numerous undesirable effects). Gentamicin significantly enhanced the generation of reactive oxygen species by silver nanoparticles.Conclusion: Generation of reactive oxygen species by Tween-coated metallic silver nanoparticles was significantly enhanced by gentamicin, confirming the hypothesis of oxidative-associated mechanism of the synergistic antibacterial effect of the gentamicin-silver nanoparticles complex.Keywords: gentamicin, silver nanoparticles, reactive oxygen species, multidrug-resistant, Staphylococcus epidermidis
format	article
author	Mazur P Skiba-Kurek I Mrowiec P Karczewska E Drożdż R
author_facet	Mazur P Skiba-Kurek I Mrowiec P Karczewska E Drożdż R
author_sort	Mazur P
title	Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis
title_short	Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis
title_full	Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis
title_fullStr	Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis
title_full_unstemmed	Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis
title_sort	synergistic ros-associated antimicrobial activity of silver nanoparticles and gentamicin against staphylococcus epidermidis
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/8a191da83dbf450e8fe9b99f332dbd21
work_keys_str_mv	AT mazurp synergisticrosassociatedantimicrobialactivityofsilvernanoparticlesandgentamicinagainststaphylococcusepidermidis AT skibakureki synergisticrosassociatedantimicrobialactivityofsilvernanoparticlesandgentamicinagainststaphylococcusepidermidis AT mrowiecp synergisticrosassociatedantimicrobialactivityofsilvernanoparticlesandgentamicinagainststaphylococcusepidermidis AT karczewskae synergisticrosassociatedantimicrobialactivityofsilvernanoparticlesandgentamicinagainststaphylococcusepidermidis AT drozdzr synergisticrosassociatedantimicrobialactivityofsilvernanoparticlesandgentamicinagainststaphylococcusepidermidis
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Synergistic ROS-Associated Antimicrobial Activity of Silver Nanoparticles and Gentamicin Against Staphylococcus epidermidis

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