Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity

Jun-ming Huang, Chen-zhong Wang, Shun-yi Lu, Zhe Wang, Zuo-qin Yan Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai, 200032, People’s Republic of ChinaCorrespondence: Zuo-qin Yan 180 Feng Lin Road, Xuhui District, Shanghai, 200032, People’s Republic of ChinaEmail yan.zuoqin...

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Autores principales:	Huang JM, Wang CZ, Lu SY, Wang Z, Yan ZQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	osteoclast oroxin b mapk nf-κb rankl osteoporosis Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8a1b3af3419142da93113e45e2c3dbf2
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spelling	oai:doaj.org-article:8a1b3af3419142da93113e45e2c3dbf22021-11-30T18:50:36ZOroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity1177-8881https://doaj.org/article/8a1b3af3419142da93113e45e2c3dbf22021-11-01T00:00:00Zhttps://www.dovepress.com/oroxin-b-attenuates-ovariectomy-induced-bone-loss-by-suppressing-osteo-peer-reviewed-fulltext-article-DDDThttps://doaj.org/toc/1177-8881Jun-ming Huang, Chen-zhong Wang, Shun-yi Lu, Zhe Wang, Zuo-qin Yan Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai, 200032, People’s Republic of ChinaCorrespondence: Zuo-qin Yan 180 Feng Lin Road, Xuhui District, Shanghai, 200032, People’s Republic of ChinaEmail yan.zuoqin@zs-hospital.sh.cnBackground: Osteoclasts are the major players in bone resorption and have always been studied in the prevention and treatment of osteoporosis. Previous studies have confirmed that a variety of flavonoids inhibit osteoporosis and improve bone health mainly through inhibiting osteoclastogenesis. Oroxin B (OB) is a flavonoid compound extracted from traditional Chinese herbal medicine Oroxylum indicum (L.) Vent, exerts potent antitumor and anti-inflammation effect, but its effect on osteoclastogensis remains unknown.Methods: We comprehensively evaluated the effect of OB on the formation and function of osteoclasts and the underling mechanism by bone marrow-derived macrophage in vitro. In vivo, we used mice ovariectomized model to verify the protective effect of OB.Results: OB was found to inhibit osteoclast formation and bone resorption function in vitro, in a dose-dependent manner and the increased osteoclastic-related genes induced by RANKL (NFATc1, c-fos, cathepsin K, RANK, MMP9 and TRAP) were also attenuated following OB treatment. Mechanistical investigation showed OB abrogated the increased phosphorylation level of MAPK and NF-κB pathway, and diminished the expression of the vital transcription factors for osteoclastogenesis. OB also prevented ovariectomy (OVX)-induced bone loss by inhibiting osteoclast formation and activity in mice.Conclusion: Our study demonstrated that OB may act as an anti-osteoporosis agent by inhibiting osteoclast maturation and attenuating bone resorption.Keywords: osteoclast, Oroxin B, MAPK, NF-κB, RANKL, osteoporosisHuang JMWang CZLu SYWang ZYan ZQDove Medical Pressarticleosteoclastoroxin bmapknf-κbranklosteoporosisTherapeutics. PharmacologyRM1-950ENDrug Design, Development and Therapy, Vol Volume 15, Pp 4811-4825 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osteoclast oroxin b mapk nf-κb rankl osteoporosis Therapeutics. Pharmacology RM1-950
spellingShingle	osteoclast oroxin b mapk nf-κb rankl osteoporosis Therapeutics. Pharmacology RM1-950 Huang JM Wang CZ Lu SY Wang Z Yan ZQ Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity
description	Jun-ming Huang, Chen-zhong Wang, Shun-yi Lu, Zhe Wang, Zuo-qin Yan Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai, 200032, People’s Republic of ChinaCorrespondence: Zuo-qin Yan 180 Feng Lin Road, Xuhui District, Shanghai, 200032, People’s Republic of ChinaEmail yan.zuoqin@zs-hospital.sh.cnBackground: Osteoclasts are the major players in bone resorption and have always been studied in the prevention and treatment of osteoporosis. Previous studies have confirmed that a variety of flavonoids inhibit osteoporosis and improve bone health mainly through inhibiting osteoclastogenesis. Oroxin B (OB) is a flavonoid compound extracted from traditional Chinese herbal medicine Oroxylum indicum (L.) Vent, exerts potent antitumor and anti-inflammation effect, but its effect on osteoclastogensis remains unknown.Methods: We comprehensively evaluated the effect of OB on the formation and function of osteoclasts and the underling mechanism by bone marrow-derived macrophage in vitro. In vivo, we used mice ovariectomized model to verify the protective effect of OB.Results: OB was found to inhibit osteoclast formation and bone resorption function in vitro, in a dose-dependent manner and the increased osteoclastic-related genes induced by RANKL (NFATc1, c-fos, cathepsin K, RANK, MMP9 and TRAP) were also attenuated following OB treatment. Mechanistical investigation showed OB abrogated the increased phosphorylation level of MAPK and NF-κB pathway, and diminished the expression of the vital transcription factors for osteoclastogenesis. OB also prevented ovariectomy (OVX)-induced bone loss by inhibiting osteoclast formation and activity in mice.Conclusion: Our study demonstrated that OB may act as an anti-osteoporosis agent by inhibiting osteoclast maturation and attenuating bone resorption.Keywords: osteoclast, Oroxin B, MAPK, NF-κB, RANKL, osteoporosis
format	article
author	Huang JM Wang CZ Lu SY Wang Z Yan ZQ
author_facet	Huang JM Wang CZ Lu SY Wang Z Yan ZQ
author_sort	Huang JM
title	Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity
title_short	Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity
title_full	Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity
title_fullStr	Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity
title_full_unstemmed	Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity
title_sort	oroxin b attenuates ovariectomy-induced bone loss by suppressing osteoclast formation and activity
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/8a1b3af3419142da93113e45e2c3dbf2
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Oroxin B Attenuates Ovariectomy-Induced Bone Loss by Suppressing Osteoclast Formation and Activity

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