Functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy

Lei Liu,1,* Hong-Jun Xie,2,* Li-Min Mu,1 Rui Liu,1 Zhan-Bo Su,1 Yi-Nuo Cui,1 Ying Xie,1 Wan-Liang Lu1 1Beijing Key Laboratory of Molecular Pharmaceutics and New Drug System, State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China; 2...

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Autores principales:	Liu L, Xie HJ, Mu LM, Liu R, Su ZB, Cui YN, Xie Y, Lu WL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Functional chlorin gold nanorods PTT/PDT cellular uptake cytotoxicity apoptosis breast cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8a3ed6f1c5dd4c58bf527dc90b5da9bd
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spelling	oai:doaj.org-article:8a3ed6f1c5dd4c58bf527dc90b5da9bd2021-12-02T06:20:18ZFunctional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy1178-2013https://doaj.org/article/8a3ed6f1c5dd4c58bf527dc90b5da9bd2018-11-01T00:00:00Zhttps://www.dovepress.com/functional-chlorin-gold-nanorods-enable-to-treat-breast-cancer-by-phot-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lei Liu,1,* Hong-Jun Xie,2,* Li-Min Mu,1 Rui Liu,1 Zhan-Bo Su,1 Yi-Nuo Cui,1 Ying Xie,1 Wan-Liang Lu1 1Beijing Key Laboratory of Molecular Pharmaceutics and New Drug System, State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China; 2Department of Medicament, College of Medicine, Tibet University, Lhasa, China *These authors contributed equally to this work Background: The existing chemo/radiotherapy fail to eliminate cancer cells due to the restriction of either drug resistance or radio tolerance. The predicament urges researchers to continuously explore alternative strategy for achieving a potent curative effect. Methods: Functional chlorin gold nanorods (Ce6-AuNR@SiO2-d-CPP) were fabricated aiming at treating breast cancer by photothermal/photodynamic therapy (PTT/PDT). The nanostructure was developed by synthesizing Au nanorods as the photothermal conversion material, and by coating the pegylated mesoporous SiO2 as the shell for entrapping photosensitizer Ce6 and for linking the D-type cell penetrating peptide (d-CPP). The function of Ce6-AuNR@SiO2-d-CPP was verified on human breast cancer MCF-7 cells and MCF-7 cells xenografts in nude mice. Results: Under combinational treatment of PTT and PDT, Ce6-AuNR@SiO2-d-CPP demonstrated a strong cytotoxicity and apoptosis inducing effects in breast cancer cells in vitro, and a robust treatment efficacy in breast cancer-bearing nude mice. The uptake mechanism involved the energy-consuming caveolin-mediated endocytosis, and Ce6-AuNR@SiO2-d-CPP in PTT/PDT mode could induce apoptosis by multiple pathways in breast cancer cells. Conclusion: Ce6-AuNR@SiO2-d-CPP demonstrated a robust efficacy in the treatment of breast cancer by photothermal/photodynamic therapy. Therefore, the present study could offer a new promising strategy to treat the refractory breast cancer. Keywords: functional chlorin gold nanorods, cell penetrating peptide, PTT/PDT, cellular uptake, cytotoxicity, apoptosisLiu LXie HJMu LMLiu RSu ZBCui YNXie YLu WLDove Medical PressarticleFunctional chlorin gold nanorodsPTT/PDTcellular uptakecytotoxicityapoptosisbreast cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 8119-8135 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Functional chlorin gold nanorods PTT/PDT cellular uptake cytotoxicity apoptosis breast cancer Medicine (General) R5-920
spellingShingle	Functional chlorin gold nanorods PTT/PDT cellular uptake cytotoxicity apoptosis breast cancer Medicine (General) R5-920 Liu L Xie HJ Mu LM Liu R Su ZB Cui YN Xie Y Lu WL Functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy
description	Lei Liu,1,* Hong-Jun Xie,2,* Li-Min Mu,1 Rui Liu,1 Zhan-Bo Su,1 Yi-Nuo Cui,1 Ying Xie,1 Wan-Liang Lu1 1Beijing Key Laboratory of Molecular Pharmaceutics and New Drug System, State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China; 2Department of Medicament, College of Medicine, Tibet University, Lhasa, China *These authors contributed equally to this work Background: The existing chemo/radiotherapy fail to eliminate cancer cells due to the restriction of either drug resistance or radio tolerance. The predicament urges researchers to continuously explore alternative strategy for achieving a potent curative effect. Methods: Functional chlorin gold nanorods (Ce6-AuNR@SiO2-d-CPP) were fabricated aiming at treating breast cancer by photothermal/photodynamic therapy (PTT/PDT). The nanostructure was developed by synthesizing Au nanorods as the photothermal conversion material, and by coating the pegylated mesoporous SiO2 as the shell for entrapping photosensitizer Ce6 and for linking the D-type cell penetrating peptide (d-CPP). The function of Ce6-AuNR@SiO2-d-CPP was verified on human breast cancer MCF-7 cells and MCF-7 cells xenografts in nude mice. Results: Under combinational treatment of PTT and PDT, Ce6-AuNR@SiO2-d-CPP demonstrated a strong cytotoxicity and apoptosis inducing effects in breast cancer cells in vitro, and a robust treatment efficacy in breast cancer-bearing nude mice. The uptake mechanism involved the energy-consuming caveolin-mediated endocytosis, and Ce6-AuNR@SiO2-d-CPP in PTT/PDT mode could induce apoptosis by multiple pathways in breast cancer cells. Conclusion: Ce6-AuNR@SiO2-d-CPP demonstrated a robust efficacy in the treatment of breast cancer by photothermal/photodynamic therapy. Therefore, the present study could offer a new promising strategy to treat the refractory breast cancer. Keywords: functional chlorin gold nanorods, cell penetrating peptide, PTT/PDT, cellular uptake, cytotoxicity, apoptosis
format	article
author	Liu L Xie HJ Mu LM Liu R Su ZB Cui YN Xie Y Lu WL
author_facet	Liu L Xie HJ Mu LM Liu R Su ZB Cui YN Xie Y Lu WL
author_sort	Liu L
title	Functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy
title_short	Functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy
title_full	Functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy
title_fullStr	Functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy
title_full_unstemmed	Functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy
title_sort	functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/8a3ed6f1c5dd4c58bf527dc90b5da9bd
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_version_	1718399946956210176

Functional chlorin gold nanorods enable to treat breast cancer by photothermal/photodynamic therapy

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