Development of an aerogenous Escherichia coli infection model in adult broiler breeders

Abstract Escherichia coli constitutes an immense challenge to the poultry industry due to its devastating effect on productivity, mortality, and carcass condemnations. To aid future studies on disease mechanisms and interventions, an aerogenous infection model was established in adult broiler breede...

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Autores principales:	Sofie Kromann, Rikke Heidemann Olsen, Anders Miki Bojesen, Henrik Elvang Jensen, Ida Thøfner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a43e9aa0440414896535e77bda2f86b
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spelling	oai:doaj.org-article:8a43e9aa0440414896535e77bda2f86b2021-12-02T18:51:28ZDevelopment of an aerogenous Escherichia coli infection model in adult broiler breeders10.1038/s41598-021-98270-82045-2322https://doaj.org/article/8a43e9aa0440414896535e77bda2f86b2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98270-8https://doaj.org/toc/2045-2322Abstract Escherichia coli constitutes an immense challenge to the poultry industry due to its devastating effect on productivity, mortality, and carcass condemnations. To aid future studies on disease mechanisms and interventions, an aerogenous infection model was established in adult broiler breeders. Hens (n = 120) were randomly allocated into six groups receiving either aerosolised E. coli or vehicle, or intratracheal E. coli or vehicle. Replication of aerosol inoculation was performed on distinct days. Alternating euthanasia time points were predetermined in order to evaluate the progression of the disease. All animals were thoroughly necropsied, and bacteriological samples were collected as well as tissues for histopathology. Birds inoculated with E. coli exhibited clinical signs and developed characteristic gross and histopathological lesions of colibacillosis, including splenic fibrinoid necrosis, folliculitis, polyserositis and impaction of parabronchi with fibrinoheterophilic exudate and necrotic debris, as well as positive in situ localisation of intralesional E. coli by immunohistochemistry. This study presents a successful development of a discriminative colibacillosis model through aerosol inoculation of adult broiler breeders. Gross and histopathological lesions characteristic of colibacillosis were established in two independent experiments.Sofie KromannRikke Heidemann OlsenAnders Miki BojesenHenrik Elvang JensenIda ThøfnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner Development of an aerogenous Escherichia coli infection model in adult broiler breeders
description	Abstract Escherichia coli constitutes an immense challenge to the poultry industry due to its devastating effect on productivity, mortality, and carcass condemnations. To aid future studies on disease mechanisms and interventions, an aerogenous infection model was established in adult broiler breeders. Hens (n = 120) were randomly allocated into six groups receiving either aerosolised E. coli or vehicle, or intratracheal E. coli or vehicle. Replication of aerosol inoculation was performed on distinct days. Alternating euthanasia time points were predetermined in order to evaluate the progression of the disease. All animals were thoroughly necropsied, and bacteriological samples were collected as well as tissues for histopathology. Birds inoculated with E. coli exhibited clinical signs and developed characteristic gross and histopathological lesions of colibacillosis, including splenic fibrinoid necrosis, folliculitis, polyserositis and impaction of parabronchi with fibrinoheterophilic exudate and necrotic debris, as well as positive in situ localisation of intralesional E. coli by immunohistochemistry. This study presents a successful development of a discriminative colibacillosis model through aerosol inoculation of adult broiler breeders. Gross and histopathological lesions characteristic of colibacillosis were established in two independent experiments.
format	article
author	Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner
author_facet	Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner
author_sort	Sofie Kromann
title	Development of an aerogenous Escherichia coli infection model in adult broiler breeders
title_short	Development of an aerogenous Escherichia coli infection model in adult broiler breeders
title_full	Development of an aerogenous Escherichia coli infection model in adult broiler breeders
title_fullStr	Development of an aerogenous Escherichia coli infection model in adult broiler breeders
title_full_unstemmed	Development of an aerogenous Escherichia coli infection model in adult broiler breeders
title_sort	development of an aerogenous escherichia coli infection model in adult broiler breeders
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8a43e9aa0440414896535e77bda2f86b
work_keys_str_mv	AT sofiekromann developmentofanaerogenousescherichiacoliinfectionmodelinadultbroilerbreeders AT rikkeheidemannolsen developmentofanaerogenousescherichiacoliinfectionmodelinadultbroilerbreeders AT andersmikibojesen developmentofanaerogenousescherichiacoliinfectionmodelinadultbroilerbreeders AT henrikelvangjensen developmentofanaerogenousescherichiacoliinfectionmodelinadultbroilerbreeders AT idathøfner developmentofanaerogenousescherichiacoliinfectionmodelinadultbroilerbreeders
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Development of an aerogenous Escherichia coli infection model in adult broiler breeders

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