Label-Free detection of antibodies using functionalised long period grating optical fibre sensors

Immunoglobulin A (IgA), IgG, and IgM immunosensors based on long period gratings (LPG) are developed in this work. An LPG has the advantages of being lightweight and small size, which provide possibility to design miniaturized immunosensors for point of care antibody tests. Poly (allylamine hydrochl...

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Autores principales:	Peizhou Wu, Liangliang Liu, Stephen P. Morgan, Ricardo Correia, Serhiy Korposh
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Optical fibre biosensor Long period grating IgA IgG IgM Optics. Light QC350-467
Acceso en línea:	https://doaj.org/article/8a44a50eff0941d985b1939c0be39ea1
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spelling	oai:doaj.org-article:8a44a50eff0941d985b1939c0be39ea12021-11-04T04:43:43ZLabel-Free detection of antibodies using functionalised long period grating optical fibre sensors2666-950110.1016/j.rio.2021.100172https://doaj.org/article/8a44a50eff0941d985b1939c0be39ea12021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666950121001188https://doaj.org/toc/2666-9501Immunoglobulin A (IgA), IgG, and IgM immunosensors based on long period gratings (LPG) are developed in this work. An LPG has the advantages of being lightweight and small size, which provide possibility to design miniaturized immunosensors for point of care antibody tests. Poly (allylamine hydrochloride) (PAH) and silica nanoparticles (SiNPs) are deposited on the surface of an LPG using a layer-by-layer method. The PAH/SiNPs matrix on the surface of LPG provides a porous surface structure, which is used to immobilise bio-receptors. Anti-IgA, anti-IgG, and anti-IgM are immobilised on the surface of three LPGs to fabricate IgA, IgG, and IgM sensors respectively. The refractive index (RI) on the surface of the LPG increases with antibody concentration, which induces wavelength shifts of the LPG’s attenuation bands. The performance tests of IgA, IgG, and IgM sensors are carried out in the ranges of 10–200 μg/ml, 1–90 μg/ml, and 10–320 μg/ml respectively, and the minimum detected concentrations achieved for all the three sensors are 10 μg/ml. The selectivity of the platform is investigated based on an IgM sensor. Preliminary results obtained in this work demonstrate that LPG-based immunosensors can achieve sufficient selectivity and suitable detection range for human serum testing.Peizhou WuLiangliang LiuStephen P. MorganRicardo CorreiaSerhiy KorposhElsevierarticleOptical fibre biosensorLong period gratingIgAIgGIgMOptics. LightQC350-467ENResults in Optics, Vol 5, Iss , Pp 100172- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Optical fibre biosensor Long period grating IgA IgG IgM Optics. Light QC350-467
spellingShingle	Optical fibre biosensor Long period grating IgA IgG IgM Optics. Light QC350-467 Peizhou Wu Liangliang Liu Stephen P. Morgan Ricardo Correia Serhiy Korposh Label-Free detection of antibodies using functionalised long period grating optical fibre sensors
description	Immunoglobulin A (IgA), IgG, and IgM immunosensors based on long period gratings (LPG) are developed in this work. An LPG has the advantages of being lightweight and small size, which provide possibility to design miniaturized immunosensors for point of care antibody tests. Poly (allylamine hydrochloride) (PAH) and silica nanoparticles (SiNPs) are deposited on the surface of an LPG using a layer-by-layer method. The PAH/SiNPs matrix on the surface of LPG provides a porous surface structure, which is used to immobilise bio-receptors. Anti-IgA, anti-IgG, and anti-IgM are immobilised on the surface of three LPGs to fabricate IgA, IgG, and IgM sensors respectively. The refractive index (RI) on the surface of the LPG increases with antibody concentration, which induces wavelength shifts of the LPG’s attenuation bands. The performance tests of IgA, IgG, and IgM sensors are carried out in the ranges of 10–200 μg/ml, 1–90 μg/ml, and 10–320 μg/ml respectively, and the minimum detected concentrations achieved for all the three sensors are 10 μg/ml. The selectivity of the platform is investigated based on an IgM sensor. Preliminary results obtained in this work demonstrate that LPG-based immunosensors can achieve sufficient selectivity and suitable detection range for human serum testing.
format	article
author	Peizhou Wu Liangliang Liu Stephen P. Morgan Ricardo Correia Serhiy Korposh
author_facet	Peizhou Wu Liangliang Liu Stephen P. Morgan Ricardo Correia Serhiy Korposh
author_sort	Peizhou Wu
title	Label-Free detection of antibodies using functionalised long period grating optical fibre sensors
title_short	Label-Free detection of antibodies using functionalised long period grating optical fibre sensors
title_full	Label-Free detection of antibodies using functionalised long period grating optical fibre sensors
title_fullStr	Label-Free detection of antibodies using functionalised long period grating optical fibre sensors
title_full_unstemmed	Label-Free detection of antibodies using functionalised long period grating optical fibre sensors
title_sort	label-free detection of antibodies using functionalised long period grating optical fibre sensors
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8a44a50eff0941d985b1939c0be39ea1
work_keys_str_mv	AT peizhouwu labelfreedetectionofantibodiesusingfunctionalisedlongperiodgratingopticalfibresensors AT liangliangliu labelfreedetectionofantibodiesusingfunctionalisedlongperiodgratingopticalfibresensors AT stephenpmorgan labelfreedetectionofantibodiesusingfunctionalisedlongperiodgratingopticalfibresensors AT ricardocorreia labelfreedetectionofantibodiesusingfunctionalisedlongperiodgratingopticalfibresensors AT serhiykorposh labelfreedetectionofantibodiesusingfunctionalisedlongperiodgratingopticalfibresensors
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Label-Free detection of antibodies using functionalised long period grating optical fibre sensors

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