Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients

Chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF) are chronic, progressive lung ailments that are characterized by distinct pathologies. Early detection biomarkers and disease mechanisms for these debilitating diseases are lacking. Extracellular vesicles (EVs), inc...

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Autores principales:	Gagandeep Kaur, Krishna Prahlad Maremanda, Michael Campos, Hitendra S. Chand, Feng Li, Nikhil Hirani, M. A. Haseeb, Dongmei Li, Irfan Rahman
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	exosomes miRNA COPD biomarker BALF lungs Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/8a4cd57c9b8d4950a48bfc37623a35ff
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spelling	oai:doaj.org-article:8a4cd57c9b8d4950a48bfc37623a35ff2021-11-11T17:16:03ZDistinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients10.3390/ijms2221118301422-00671661-6596https://doaj.org/article/8a4cd57c9b8d4950a48bfc37623a35ff2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11830https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF) are chronic, progressive lung ailments that are characterized by distinct pathologies. Early detection biomarkers and disease mechanisms for these debilitating diseases are lacking. Extracellular vesicles (EVs), including exosomes, are small, lipid-bound vesicles attributed to carry proteins, lipids, and RNA molecules to facilitate cell-to-cell communication under normal and diseased conditions. Exosomal miRNAs have been studied in relation to many diseases. However, there is little to no knowledge regarding the miRNA population of bronchoalveolar lavage fluid (BALF) or the lung-tissue-derived exosomes in COPD and IPF. Here, we determined and compared the miRNA profiles of BALF- and lung-tissue-derived exosomes of healthy non-smokers, smokers, and patients with COPD or IPF in independent cohorts. Results: Exosome characterization using NanoSight particle tracking and TEM demonstrated that the BALF-derived exosomes were ~89.85 nm in size with a yield of ~2.95 × 10<sup>10</sup> particles/mL in concentration. Lung-derived exosomes were larger in size (~146.04 nm) with a higher yield of ~2.38 × 10<sup>11</sup> particles/mL. NGS results identified three differentially expressed miRNAs in the BALF, while there was one in the lung-derived exosomes from COPD patients as compared to healthy non-smokers. Of these, miR-122-5p was three- or five-fold downregulated among the lung-tissue-derived exosomes of COPD patients as compared to healthy non-smokers and smokers, respectively. Interestingly, there were a large number (55) of differentially expressed miRNAs in the lung-tissue-derived exosomes of IPF patients compared to non-smoking controls. Conclusions: Overall, we identified lung-specific miRNAs associated with chronic lung diseases that can serve as potential biomarkers or therapeutic targets.Gagandeep KaurKrishna Prahlad MaremandaMichael CamposHitendra S. ChandFeng LiNikhil HiraniM. A. HaseebDongmei LiIrfan RahmanMDPI AGarticleexosomesmiRNACOPDbiomarkerBALFlungsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11830, p 11830 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	exosomes miRNA COPD biomarker BALF lungs Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	exosomes miRNA COPD biomarker BALF lungs Biology (General) QH301-705.5 Chemistry QD1-999 Gagandeep Kaur Krishna Prahlad Maremanda Michael Campos Hitendra S. Chand Feng Li Nikhil Hirani M. A. Haseeb Dongmei Li Irfan Rahman Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients
description	Chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF) are chronic, progressive lung ailments that are characterized by distinct pathologies. Early detection biomarkers and disease mechanisms for these debilitating diseases are lacking. Extracellular vesicles (EVs), including exosomes, are small, lipid-bound vesicles attributed to carry proteins, lipids, and RNA molecules to facilitate cell-to-cell communication under normal and diseased conditions. Exosomal miRNAs have been studied in relation to many diseases. However, there is little to no knowledge regarding the miRNA population of bronchoalveolar lavage fluid (BALF) or the lung-tissue-derived exosomes in COPD and IPF. Here, we determined and compared the miRNA profiles of BALF- and lung-tissue-derived exosomes of healthy non-smokers, smokers, and patients with COPD or IPF in independent cohorts. Results: Exosome characterization using NanoSight particle tracking and TEM demonstrated that the BALF-derived exosomes were ~89.85 nm in size with a yield of ~2.95 × 10<sup>10</sup> particles/mL in concentration. Lung-derived exosomes were larger in size (~146.04 nm) with a higher yield of ~2.38 × 10<sup>11</sup> particles/mL. NGS results identified three differentially expressed miRNAs in the BALF, while there was one in the lung-derived exosomes from COPD patients as compared to healthy non-smokers. Of these, miR-122-5p was three- or five-fold downregulated among the lung-tissue-derived exosomes of COPD patients as compared to healthy non-smokers and smokers, respectively. Interestingly, there were a large number (55) of differentially expressed miRNAs in the lung-tissue-derived exosomes of IPF patients compared to non-smoking controls. Conclusions: Overall, we identified lung-specific miRNAs associated with chronic lung diseases that can serve as potential biomarkers or therapeutic targets.
format	article
author	Gagandeep Kaur Krishna Prahlad Maremanda Michael Campos Hitendra S. Chand Feng Li Nikhil Hirani M. A. Haseeb Dongmei Li Irfan Rahman
author_facet	Gagandeep Kaur Krishna Prahlad Maremanda Michael Campos Hitendra S. Chand Feng Li Nikhil Hirani M. A. Haseeb Dongmei Li Irfan Rahman
author_sort	Gagandeep Kaur
title	Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients
title_short	Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients
title_full	Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients
title_fullStr	Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients
title_full_unstemmed	Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients
title_sort	distinct exosomal mirna profiles from balf and lung tissue of copd and ipf patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8a4cd57c9b8d4950a48bfc37623a35ff
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Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF Patients

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