Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown...

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Autores principales:	Tijana Kolarević, Danijel D. Milinčić, Tatjana Vujović, Uroš M. Gašić, Ljiljana Prokić, Aleksandar Ž. Kostić, Radosav Cerović, Sladjana P. Stanojevic, Živoslav Lj. Tešić, Mirjana B. Pešić
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	open-field plants tissue culture plants in vitro callus culture <i>Rubus</i> subg. <i>Rubus</i> Watson <i>Vaccinium corymbosum</i> L. phenolics Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/8a57349fc30d44f19a87f44ef13447e2
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spelling	oai:doaj.org-article:8a57349fc30d44f19a87f44ef13447e22021-11-25T17:46:57ZPhenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry10.3390/horticulturae71104202311-7524https://doaj.org/article/8a57349fc30d44f19a87f44ef13447e22021-10-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/420https://doaj.org/toc/2311-7524The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA<sub>3</sub> (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-<i>O</i>-glucoside and quercetin-3-<i>O</i>-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS<sup>•<b><i>+</i></b></sup> and DPPH<sup>•</sup> scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS<sup>•<b><i>+</i></b></sup> and ten to seventeen times lower DPPH<sup>•</sup> scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.Tijana KolarevićDanijel D. MilinčićTatjana VujovićUroš M. GašićLjiljana ProkićAleksandar Ž. KostićRadosav CerovićSladjana P. StanojevicŽivoslav Lj. TešićMirjana B. PešićMDPI AGarticleopen-field plantstissue culture plantsin vitro callus culture<i>Rubus</i> subg. <i>Rubus</i> Watson<i>Vaccinium corymbosum</i> L.phenolicsPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 420, p 420 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	open-field plants tissue culture plants in vitro callus culture <i>Rubus</i> subg. <i>Rubus</i> Watson <i>Vaccinium corymbosum</i> L. phenolics Plant culture SB1-1110
spellingShingle	open-field plants tissue culture plants in vitro callus culture <i>Rubus</i> subg. <i>Rubus</i> Watson <i>Vaccinium corymbosum</i> L. phenolics Plant culture SB1-1110 Tijana Kolarević Danijel D. Milinčić Tatjana Vujović Uroš M. Gašić Ljiljana Prokić Aleksandar Ž. Kostić Radosav Cerović Sladjana P. Stanojevic Živoslav Lj. Tešić Mirjana B. Pešić Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
description	The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA<sub>3</sub> (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-<i>O</i>-glucoside and quercetin-3-<i>O</i>-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS<sup>•<b><i>+</i></b></sup> and DPPH<sup>•</sup> scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS<sup>•<b><i>+</i></b></sup> and ten to seventeen times lower DPPH<sup>•</sup> scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.
format	article
author	Tijana Kolarević Danijel D. Milinčić Tatjana Vujović Uroš M. Gašić Ljiljana Prokić Aleksandar Ž. Kostić Radosav Cerović Sladjana P. Stanojevic Živoslav Lj. Tešić Mirjana B. Pešić
author_facet	Tijana Kolarević Danijel D. Milinčić Tatjana Vujović Uroš M. Gašić Ljiljana Prokić Aleksandar Ž. Kostić Radosav Cerović Sladjana P. Stanojevic Živoslav Lj. Tešić Mirjana B. Pešić
author_sort	Tijana Kolarević
title	Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
title_short	Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
title_full	Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
title_fullStr	Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
title_full_unstemmed	Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
title_sort	phenolic compounds and antioxidant properties of field-grown and in vitro leaves, and calluses in blackberry and blueberry
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8a57349fc30d44f19a87f44ef13447e2
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Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

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