Fractionated photothermal antitumor therapy with multidye nanoparticles

Luke G Gutwein1, Amit K Singh2, Megan A Hahn2, Michael C Rule3, Jacquelyn A Knapik4, Brij M Moudgil2, Scott C Brown2, Stephen R Grobmyer11Division of Surgical Oncology, Department of Surgery, College of Medicine, 2Particle Engineering Research Center, 3Cell and Tissue Analysis Core, McKnight Brain I...

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Autores principales:	Gutwein LG, Singh AK, Hahn MA, Rule MC, Knapik JA, Moudgil BM, Brown SC, Grobmyer SR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8a60b936b3044511a216ac132c1420e2
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spelling	oai:doaj.org-article:8a60b936b3044511a216ac132c1420e22021-12-02T07:20:23ZFractionated photothermal antitumor therapy with multidye nanoparticles1176-91141178-2013https://doaj.org/article/8a60b936b3044511a216ac132c1420e22012-01-01T00:00:00Zhttp://www.dovepress.com/fractionated-photothermal-antitumor-therapy-with-multidye-nanoparticle-a9105https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Luke G Gutwein1, Amit K Singh2, Megan A Hahn2, Michael C Rule3, Jacquelyn A Knapik4, Brij M Moudgil2, Scott C Brown2, Stephen R Grobmyer11Division of Surgical Oncology, Department of Surgery, College of Medicine, 2Particle Engineering Research Center, 3Cell and Tissue Analysis Core, McKnight Brain Institute, 4Department of Pathology, University of Florida, Gainesville, FL, USAPurpose: Photothermal therapy is an emerging cancer treatment paradigm which involves highly localized heating and killing of tumor cells, due to the presence of nanomaterials that can strongly absorb near-infrared (NIR) light. In addition to having deep penetration depths in tissue, NIR light is innocuous to normal cells. Little is known currently about the fate of nanomaterials post photothermal ablation and the implications thereof. The purpose of this investigation was to define the intratumoral fate of nanoparticles (NPs) after photothermal therapy in vivo and characterize the use of novel multidye theranostic NPs (MDT-NPs) for fractionated photothermal antitumor therapy.Methods: The photothermal and fluorescent properties of MDT-NPs were first characterized. To investigate the fate of nanomaterials following photothermal ablation in vivo, novel MDT-NPs and a murine mammary tumor model were used. Intratumoral injection of MDT-NPs and real-time fluorescence imaging before and after fractionated photothermal therapy was performed to study the intratumoral fate of MDT-NPs. Gross tumor and histological changes were made comparing MDT-NP treated and control tumor-bearing mice.Results: The dual dye-loaded mesoporous NPs (ie, MDT-NPs; circa 100 nm) retained both their NIR absorbing and NIR fluorescent capabilities after photoactivation. In vivo MDT-NPs remained localized in the intratumoral position after photothermal ablation. With fractionated photothermal therapy, there was significant treatment effect observed macroscopically (P = 0.026) in experimental tumor-bearing mice compared to control treated tumor-bearing mice.Conclusion: Fractionated photothermal therapy for cancer represents a new therapeutic paradigm enabled by the application of novel functional nanomaterials. MDT-NPs may advance clinical treatment of cancer by enabling fractionated real-time image guided photothermal therapy.Keywords: fluorescence, mesoporous silica, biodistribution, photothermal ablation, live animal imaging, near-infrared nanoparticle, breast cancerGutwein LGSingh AKHahn MARule MCKnapik JAMoudgil BMBrown SCGrobmyer SRDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 351-357 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Gutwein LG Singh AK Hahn MA Rule MC Knapik JA Moudgil BM Brown SC Grobmyer SR Fractionated photothermal antitumor therapy with multidye nanoparticles
description	Luke G Gutwein1, Amit K Singh2, Megan A Hahn2, Michael C Rule3, Jacquelyn A Knapik4, Brij M Moudgil2, Scott C Brown2, Stephen R Grobmyer11Division of Surgical Oncology, Department of Surgery, College of Medicine, 2Particle Engineering Research Center, 3Cell and Tissue Analysis Core, McKnight Brain Institute, 4Department of Pathology, University of Florida, Gainesville, FL, USAPurpose: Photothermal therapy is an emerging cancer treatment paradigm which involves highly localized heating and killing of tumor cells, due to the presence of nanomaterials that can strongly absorb near-infrared (NIR) light. In addition to having deep penetration depths in tissue, NIR light is innocuous to normal cells. Little is known currently about the fate of nanomaterials post photothermal ablation and the implications thereof. The purpose of this investigation was to define the intratumoral fate of nanoparticles (NPs) after photothermal therapy in vivo and characterize the use of novel multidye theranostic NPs (MDT-NPs) for fractionated photothermal antitumor therapy.Methods: The photothermal and fluorescent properties of MDT-NPs were first characterized. To investigate the fate of nanomaterials following photothermal ablation in vivo, novel MDT-NPs and a murine mammary tumor model were used. Intratumoral injection of MDT-NPs and real-time fluorescence imaging before and after fractionated photothermal therapy was performed to study the intratumoral fate of MDT-NPs. Gross tumor and histological changes were made comparing MDT-NP treated and control tumor-bearing mice.Results: The dual dye-loaded mesoporous NPs (ie, MDT-NPs; circa 100 nm) retained both their NIR absorbing and NIR fluorescent capabilities after photoactivation. In vivo MDT-NPs remained localized in the intratumoral position after photothermal ablation. With fractionated photothermal therapy, there was significant treatment effect observed macroscopically (P = 0.026) in experimental tumor-bearing mice compared to control treated tumor-bearing mice.Conclusion: Fractionated photothermal therapy for cancer represents a new therapeutic paradigm enabled by the application of novel functional nanomaterials. MDT-NPs may advance clinical treatment of cancer by enabling fractionated real-time image guided photothermal therapy.Keywords: fluorescence, mesoporous silica, biodistribution, photothermal ablation, live animal imaging, near-infrared nanoparticle, breast cancer
format	article
author	Gutwein LG Singh AK Hahn MA Rule MC Knapik JA Moudgil BM Brown SC Grobmyer SR
author_facet	Gutwein LG Singh AK Hahn MA Rule MC Knapik JA Moudgil BM Brown SC Grobmyer SR
author_sort	Gutwein LG
title	Fractionated photothermal antitumor therapy with multidye nanoparticles
title_short	Fractionated photothermal antitumor therapy with multidye nanoparticles
title_full	Fractionated photothermal antitumor therapy with multidye nanoparticles
title_fullStr	Fractionated photothermal antitumor therapy with multidye nanoparticles
title_full_unstemmed	Fractionated photothermal antitumor therapy with multidye nanoparticles
title_sort	fractionated photothermal antitumor therapy with multidye nanoparticles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/8a60b936b3044511a216ac132c1420e2
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Fractionated photothermal antitumor therapy with multidye nanoparticles

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