The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule

Abstract Several anti-cancer therapies target the epidermal growth factor receptor (EGFR). Radionuclide imaging of EGFR expression in tumours may aid in selection of optimal cancer therapy. The 111In-labelled DOTA-conjugated ZEGFR:2377 Affibody molecule was successfully used for imaging of EGFR-expr...

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Autores principales:	Javad Garousi, Ken G. Andersson, Johan H. Dam, Birgitte B. Olsen, Bogdan Mitran, Anna Orlova, Jos Buijs, Stefan Ståhl, John Löfblom, Helge Thisgaard, Vladimir Tolmachev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a636f58f2cd44c59a45fa82c0cdd9f2
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spelling	oai:doaj.org-article:8a636f58f2cd44c59a45fa82c0cdd9f22021-12-02T11:52:24ZThe use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule10.1038/s41598-017-05700-72045-2322https://doaj.org/article/8a636f58f2cd44c59a45fa82c0cdd9f22017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05700-7https://doaj.org/toc/2045-2322Abstract Several anti-cancer therapies target the epidermal growth factor receptor (EGFR). Radionuclide imaging of EGFR expression in tumours may aid in selection of optimal cancer therapy. The 111In-labelled DOTA-conjugated ZEGFR:2377 Affibody molecule was successfully used for imaging of EGFR-expressing xenografts in mice. An optimal combination of radionuclide, chelator and targeting protein may further improve the contrast of radionuclide imaging. The aim of this study was to evaluate the targeting properties of radiocobalt-labelled DOTA-ZEGFR:2377. DOTA-ZEGFR:2377 was labelled with 57Co (T1/2 = 271.8 d), 55Co (T1/2 = 17.5 h), and, for comparison, with the positron-emitting radionuclide 68Ga (T1/2 = 67.6 min) with preserved specificity of binding to EGFR-expressing A431 cells. The long-lived cobalt radioisotope 57Co was used in animal studies. Both 57Co-DOTA-ZEGFR:2377 and 68Ga-DOTA-ZEGFR:2377 demonstrated EGFR-specific accumulation in A431 xenografts and EGFR-expressing tissues in mice. Tumour-to-organ ratios for the radiocobalt-labelled DOTA-ZEGFR:2377 were significantly higher than for the gallium-labelled counterpart already at 3 h after injection. Importantly, 57Co-DOTA-ZEGFR:2377 demonstrated a tumour-to-liver ratio of 3, which is 7-fold higher than the tumour-to-liver ratio for 68Ga-DOTA-ZEGFR:2377. The results of this study suggest that the positron-emitting cobalt isotope 55Co would be an optimal label for DOTA-ZEGFR:2377 and further development should concentrate on this radionuclide as a label.Javad GarousiKen G. AnderssonJohan H. DamBirgitte B. OlsenBogdan MitranAnna OrlovaJos BuijsStefan StåhlJohn LöfblomHelge ThisgaardVladimir TolmachevNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Javad Garousi Ken G. Andersson Johan H. Dam Birgitte B. Olsen Bogdan Mitran Anna Orlova Jos Buijs Stefan Ståhl John Löfblom Helge Thisgaard Vladimir Tolmachev The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule
description	Abstract Several anti-cancer therapies target the epidermal growth factor receptor (EGFR). Radionuclide imaging of EGFR expression in tumours may aid in selection of optimal cancer therapy. The 111In-labelled DOTA-conjugated ZEGFR:2377 Affibody molecule was successfully used for imaging of EGFR-expressing xenografts in mice. An optimal combination of radionuclide, chelator and targeting protein may further improve the contrast of radionuclide imaging. The aim of this study was to evaluate the targeting properties of radiocobalt-labelled DOTA-ZEGFR:2377. DOTA-ZEGFR:2377 was labelled with 57Co (T1/2 = 271.8 d), 55Co (T1/2 = 17.5 h), and, for comparison, with the positron-emitting radionuclide 68Ga (T1/2 = 67.6 min) with preserved specificity of binding to EGFR-expressing A431 cells. The long-lived cobalt radioisotope 57Co was used in animal studies. Both 57Co-DOTA-ZEGFR:2377 and 68Ga-DOTA-ZEGFR:2377 demonstrated EGFR-specific accumulation in A431 xenografts and EGFR-expressing tissues in mice. Tumour-to-organ ratios for the radiocobalt-labelled DOTA-ZEGFR:2377 were significantly higher than for the gallium-labelled counterpart already at 3 h after injection. Importantly, 57Co-DOTA-ZEGFR:2377 demonstrated a tumour-to-liver ratio of 3, which is 7-fold higher than the tumour-to-liver ratio for 68Ga-DOTA-ZEGFR:2377. The results of this study suggest that the positron-emitting cobalt isotope 55Co would be an optimal label for DOTA-ZEGFR:2377 and further development should concentrate on this radionuclide as a label.
format	article
author	Javad Garousi Ken G. Andersson Johan H. Dam Birgitte B. Olsen Bogdan Mitran Anna Orlova Jos Buijs Stefan Ståhl John Löfblom Helge Thisgaard Vladimir Tolmachev
author_facet	Javad Garousi Ken G. Andersson Johan H. Dam Birgitte B. Olsen Bogdan Mitran Anna Orlova Jos Buijs Stefan Ståhl John Löfblom Helge Thisgaard Vladimir Tolmachev
author_sort	Javad Garousi
title	The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule
title_short	The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule
title_full	The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule
title_fullStr	The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule
title_full_unstemmed	The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule
title_sort	use of radiocobalt as a label improves imaging of egfr using dota-conjugated affibody molecule
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8a636f58f2cd44c59a45fa82c0cdd9f2
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The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule

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