Single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field

Abstract Corticofugal projection neurons are key components in connecting the neocortex and the subcortical regions. In the barrel field, these neurons have various projection targets and play crucial roles in the rodent whisker sensorimotor system. However, the projection features of corticofugal p...

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Autores principales:	Congdi Guo, Jie Peng, Yalun Zhang, Anan Li, Yuxin Li, Jing Yuan, Xiaofeng Xu, Miao Ren, Hui Gong, Shangbin Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a6bff68765943a48b3ef387f93f3ee2
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spelling	oai:doaj.org-article:8a6bff68765943a48b3ef387f93f3ee22021-12-02T15:05:17ZSingle-axon level morphological analysis of corticofugal projection neurons in mouse barrel field10.1038/s41598-017-03000-82045-2322https://doaj.org/article/8a6bff68765943a48b3ef387f93f3ee22017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03000-8https://doaj.org/toc/2045-2322Abstract Corticofugal projection neurons are key components in connecting the neocortex and the subcortical regions. In the barrel field, these neurons have various projection targets and play crucial roles in the rodent whisker sensorimotor system. However, the projection features of corticofugal projection neurons at the single-axon level are far from comprehensive elucidation. Based on a brain-wide positioning system with high-resolution imaging for Thy1-GFP M-line mice brains, we reconstructed and analyzed more than one hundred corticofugal projection neurons in both layer V and VI of barrel cortex. The dual-color imaging made it possible to locate the neurons’ somata, trace their corresponding dendrites and axons and then distinguish the neurons as L5 type I/II or L6 type. The corticofugal projection pattern showed significant diversity across individual neurons. Usually, the L5 type I neurons have greater multi-region projection potential. The thalamus and the midbrain are the most frequent projection targets among the investigated multidirectional projection neurons, and the hypothalamus is particularly unique in that it only appears in multidirectional projection situations. Statistically, the average branch length of apical dendrites in multi-region projection groups is larger than that of single-region projection groups. This study demonstrated a single-axon-level analysis for barrel corticofugal projection neurons, which could provide a micro-anatomical basis for interpreting whisker sensorimotor circuit function.Congdi GuoJie PengYalun ZhangAnan LiYuxin LiJing YuanXiaofeng XuMiao RenHui GongShangbin ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Congdi Guo Jie Peng Yalun Zhang Anan Li Yuxin Li Jing Yuan Xiaofeng Xu Miao Ren Hui Gong Shangbin Chen Single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field
description	Abstract Corticofugal projection neurons are key components in connecting the neocortex and the subcortical regions. In the barrel field, these neurons have various projection targets and play crucial roles in the rodent whisker sensorimotor system. However, the projection features of corticofugal projection neurons at the single-axon level are far from comprehensive elucidation. Based on a brain-wide positioning system with high-resolution imaging for Thy1-GFP M-line mice brains, we reconstructed and analyzed more than one hundred corticofugal projection neurons in both layer V and VI of barrel cortex. The dual-color imaging made it possible to locate the neurons’ somata, trace their corresponding dendrites and axons and then distinguish the neurons as L5 type I/II or L6 type. The corticofugal projection pattern showed significant diversity across individual neurons. Usually, the L5 type I neurons have greater multi-region projection potential. The thalamus and the midbrain are the most frequent projection targets among the investigated multidirectional projection neurons, and the hypothalamus is particularly unique in that it only appears in multidirectional projection situations. Statistically, the average branch length of apical dendrites in multi-region projection groups is larger than that of single-region projection groups. This study demonstrated a single-axon-level analysis for barrel corticofugal projection neurons, which could provide a micro-anatomical basis for interpreting whisker sensorimotor circuit function.
format	article
author	Congdi Guo Jie Peng Yalun Zhang Anan Li Yuxin Li Jing Yuan Xiaofeng Xu Miao Ren Hui Gong Shangbin Chen
author_facet	Congdi Guo Jie Peng Yalun Zhang Anan Li Yuxin Li Jing Yuan Xiaofeng Xu Miao Ren Hui Gong Shangbin Chen
author_sort	Congdi Guo
title	Single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field
title_short	Single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field
title_full	Single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field
title_fullStr	Single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field
title_full_unstemmed	Single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field
title_sort	single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8a6bff68765943a48b3ef387f93f3ee2
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Single-axon level morphological analysis of corticofugal projection neurons in mouse barrel field

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