Using Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry

(1) Background: Acute promyelocytic leukemia is curable, but bleeding complications still provoke a high early mortality. Therefore, a fast diagnosis is needed for timely starting treatment. We developed a diagnostic algorithm using flow cytometric features for discrimination between acute promyeloc...

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Autores principales:	Vitória Ceni-Silva, Kátia Pagnano, Gislaine Duarte, Marina Pellegrini, Bruno Duarte, Konradin Metze, Irene Lorand-Metze
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	acute promyelocytic leukemia diagnosis flow cytometry Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8a6f91922c1c49ea99cffd384f7a44ce
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spelling	oai:doaj.org-article:8a6f91922c1c49ea99cffd384f7a44ce2021-11-25T17:20:32ZUsing Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry10.3390/diagnostics111119882075-4418https://doaj.org/article/8a6f91922c1c49ea99cffd384f7a44ce2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/1988https://doaj.org/toc/2075-4418(1) Background: Acute promyelocytic leukemia is curable, but bleeding complications still provoke a high early mortality. Therefore, a fast diagnosis is needed for timely starting treatment. We developed a diagnostic algorithm using flow cytometric features for discrimination between acute promyelocytic leukemia (APL) and other types of acute myeloid leukemias (AML). (2) Methods: we analyzed newly diagnosed AMLs where immunophenotyping was performed at diagnosis by an 8-color protocol. The mean fluorescence intensity (MFI) of each antigen used was assessed, and those best separating APL from other types of AML were obtained by a discriminant analysis. Phenotypic characteristics of myeloblasts of normal bone marrow were used as controls. (3) Results: 24 cases of APL and 56 cases of other primary AMLs entered the study. Among non-APL AMLs, 4 had fms-related tyrosine kinase 3 gene internal tandem duplications (FLT3-ITD) mutation, 2 had nucleophosmin (NPM1) and 10 had both mutations. SSC (<i>p</i> < 0.0001), HLA-DR (<i>p</i> < 0.0001), CD13 (<i>p</i> = 0.001), CD64 (<i>p</i> = 0.004) and CD33 (<i>p</i> = 0.002) were differentially expressed, but this was not the case for CD34 (50% of non-APLs had a low expression). In the discriminant analysis, the best differentiation was achieved with SSC and HLA-DR discriminating 91.25% of the patients. (4) Conclusion: MFC could differentiate APL from non-APL AML in the majority of the cases.Vitória Ceni-SilvaKátia PagnanoGislaine DuarteMarina PellegriniBruno DuarteKonradin MetzeIrene Lorand-MetzeMDPI AGarticleacute promyelocytic leukemiadiagnosisflow cytometryMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 1988, p 1988 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acute promyelocytic leukemia diagnosis flow cytometry Medicine (General) R5-920
spellingShingle	acute promyelocytic leukemia diagnosis flow cytometry Medicine (General) R5-920 Vitória Ceni-Silva Kátia Pagnano Gislaine Duarte Marina Pellegrini Bruno Duarte Konradin Metze Irene Lorand-Metze Using Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry
description	(1) Background: Acute promyelocytic leukemia is curable, but bleeding complications still provoke a high early mortality. Therefore, a fast diagnosis is needed for timely starting treatment. We developed a diagnostic algorithm using flow cytometric features for discrimination between acute promyelocytic leukemia (APL) and other types of acute myeloid leukemias (AML). (2) Methods: we analyzed newly diagnosed AMLs where immunophenotyping was performed at diagnosis by an 8-color protocol. The mean fluorescence intensity (MFI) of each antigen used was assessed, and those best separating APL from other types of AML were obtained by a discriminant analysis. Phenotypic characteristics of myeloblasts of normal bone marrow were used as controls. (3) Results: 24 cases of APL and 56 cases of other primary AMLs entered the study. Among non-APL AMLs, 4 had fms-related tyrosine kinase 3 gene internal tandem duplications (FLT3-ITD) mutation, 2 had nucleophosmin (NPM1) and 10 had both mutations. SSC (<i>p</i> < 0.0001), HLA-DR (<i>p</i> < 0.0001), CD13 (<i>p</i> = 0.001), CD64 (<i>p</i> = 0.004) and CD33 (<i>p</i> = 0.002) were differentially expressed, but this was not the case for CD34 (50% of non-APLs had a low expression). In the discriminant analysis, the best differentiation was achieved with SSC and HLA-DR discriminating 91.25% of the patients. (4) Conclusion: MFC could differentiate APL from non-APL AML in the majority of the cases.
format	article
author	Vitória Ceni-Silva Kátia Pagnano Gislaine Duarte Marina Pellegrini Bruno Duarte Konradin Metze Irene Lorand-Metze
author_facet	Vitória Ceni-Silva Kátia Pagnano Gislaine Duarte Marina Pellegrini Bruno Duarte Konradin Metze Irene Lorand-Metze
author_sort	Vitória Ceni-Silva
title	Using Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry
title_short	Using Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry
title_full	Using Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry
title_fullStr	Using Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry
title_full_unstemmed	Using Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry
title_sort	using antigen expression of leukemic cells for a fast screening of acute promyelocytic leukemia by flow cytometry
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8a6f91922c1c49ea99cffd384f7a44ce
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Using Antigen Expression of Leukemic Cells for a Fast Screening of Acute Promyelocytic Leukemia by Flow Cytometry

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