Amphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark

The present study evaluated the wound healing, anthelmintic and antioxidant potentials of crude methanol extracts and fractions (petroleum ether, ethyl acetate and methanol) of the leaves and stem bark of Amphimas pterocarpoides. Wound healing activity was determined by the dermal excision model in...

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Autores principales:	Evelyn Asante-Kwatia, Silas Adjei, Yakubu Jibira, Lord Gyimah, George Adjei-Hinneh, Isaac Kingsley Amponsah, Abraham Yeboah Mensah
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Amphimas pterocarpoides Dermal excision Worms Oxidative stress HPLC Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/8a70d3a4e4904fd895131b4419d1bd42
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spelling	oai:doaj.org-article:8a70d3a4e4904fd895131b4419d1bd422021-12-02T05:02:19ZAmphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark2405-844010.1016/j.heliyon.2021.e08261https://doaj.org/article/8a70d3a4e4904fd895131b4419d1bd422021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021023641https://doaj.org/toc/2405-8440The present study evaluated the wound healing, anthelmintic and antioxidant potentials of crude methanol extracts and fractions (petroleum ether, ethyl acetate and methanol) of the leaves and stem bark of Amphimas pterocarpoides. Wound healing activity was determined by the dermal excision model in rats; anthelmintic activity was evaluated by the adult worm motility test using the adult Indian worm, Pheretima postuma. Total flavonoid, phenolic content and antioxidant activity were assessed by the aluminum chloride colorimetric, Folin Ciocalteu, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and total antioxidant capacity (TAC) assays respectively. HPLC/UV fingerprints were developed for quality control. The maximum amount of phenolics and flavonoids were detected in the methanol fractions of the stem bark (225.0 ± 20.0 mg/g gallic acid equivalent (GAE) and 201.0 ± 1.41 mg/g quercetin equivalent (QCE) respectively) and leaves (84.54 ± 1.36 mg/g GAE and 130.7 ± 1.71 mg/g QCE, respectively). Both leaf and bark displayed remarkable free radical scavenging and TAC with the highest effect given by the methanol fractions. Significant (p < 0.05) wound contraction was achieved by topical application of the leaf (APL) and stem bark (APS) ointments (5–15%) with >90 % wound surface closure for 1% silver sulphadiazine, APS 15% and APL 10% treated groups by day 15. APL and APS demonstrated a concentration- and time-dependent paralysis and mortality of the P. posthuma with APL (6.25 mg/mL) causing worm paralysis at 82.60 min and death at 93 min, better than 10 mg/mL albendazole (paralysis at 76.30 min; death at 117 min). Tannins, triterpenoids, phytosterols, flavonoids, saponins and coumarins were detected in the leaves and bark. The results have proven the potential of A. pterocarpoides as a wound healing and anthelmintic agent, giving scientific credence to its use in traditional medicine.Evelyn Asante-KwatiaSilas AdjeiYakubu JibiraLord GyimahGeorge Adjei-HinnehIsaac Kingsley AmponsahAbraham Yeboah MensahElsevierarticleAmphimas pterocarpoidesDermal excisionWormsOxidative stressHPLCScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08261- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Amphimas pterocarpoides Dermal excision Worms Oxidative stress HPLC Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Amphimas pterocarpoides Dermal excision Worms Oxidative stress HPLC Science (General) Q1-390 Social sciences (General) H1-99 Evelyn Asante-Kwatia Silas Adjei Yakubu Jibira Lord Gyimah George Adjei-Hinneh Isaac Kingsley Amponsah Abraham Yeboah Mensah Amphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark
description	The present study evaluated the wound healing, anthelmintic and antioxidant potentials of crude methanol extracts and fractions (petroleum ether, ethyl acetate and methanol) of the leaves and stem bark of Amphimas pterocarpoides. Wound healing activity was determined by the dermal excision model in rats; anthelmintic activity was evaluated by the adult worm motility test using the adult Indian worm, Pheretima postuma. Total flavonoid, phenolic content and antioxidant activity were assessed by the aluminum chloride colorimetric, Folin Ciocalteu, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and total antioxidant capacity (TAC) assays respectively. HPLC/UV fingerprints were developed for quality control. The maximum amount of phenolics and flavonoids were detected in the methanol fractions of the stem bark (225.0 ± 20.0 mg/g gallic acid equivalent (GAE) and 201.0 ± 1.41 mg/g quercetin equivalent (QCE) respectively) and leaves (84.54 ± 1.36 mg/g GAE and 130.7 ± 1.71 mg/g QCE, respectively). Both leaf and bark displayed remarkable free radical scavenging and TAC with the highest effect given by the methanol fractions. Significant (p < 0.05) wound contraction was achieved by topical application of the leaf (APL) and stem bark (APS) ointments (5–15%) with >90 % wound surface closure for 1% silver sulphadiazine, APS 15% and APL 10% treated groups by day 15. APL and APS demonstrated a concentration- and time-dependent paralysis and mortality of the P. posthuma with APL (6.25 mg/mL) causing worm paralysis at 82.60 min and death at 93 min, better than 10 mg/mL albendazole (paralysis at 76.30 min; death at 117 min). Tannins, triterpenoids, phytosterols, flavonoids, saponins and coumarins were detected in the leaves and bark. The results have proven the potential of A. pterocarpoides as a wound healing and anthelmintic agent, giving scientific credence to its use in traditional medicine.
format	article
author	Evelyn Asante-Kwatia Silas Adjei Yakubu Jibira Lord Gyimah George Adjei-Hinneh Isaac Kingsley Amponsah Abraham Yeboah Mensah
author_facet	Evelyn Asante-Kwatia Silas Adjei Yakubu Jibira Lord Gyimah George Adjei-Hinneh Isaac Kingsley Amponsah Abraham Yeboah Mensah
author_sort	Evelyn Asante-Kwatia
title	Amphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark
title_short	Amphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark
title_full	Amphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark
title_fullStr	Amphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark
title_full_unstemmed	Amphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark
title_sort	amphimas pterocarpoides harms.: an evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8a70d3a4e4904fd895131b4419d1bd42
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Amphimas pterocarpoides harms.: An Evaluation of flavonoid and phenolic contents, wound healing, anthelmintic and antioxidant activities of the leaves and stem bark

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