Long non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model

Abstract Long non-coding RNAs (lncRNAs) have critical regulatory roles in protein-coding gene expression. Aberrant expression profiles of lncRNAs have been observed in various human diseases. In this study, we investigated transcriptome profiles in lung tissues of chronic cigarette smoke (CS)-induce...

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Autores principales:	Haiyun Zhang, Dejun Sun, Defu Li, Zeguang Zheng, Jingyi Xu, Xue Liang, Chenting Zhang, Sheng Wang, Jian Wang, Wenju Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8a7252ef18474be9b2a1bd0fa497bad5
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spelling	oai:doaj.org-article:8a7252ef18474be9b2a1bd0fa497bad52021-12-02T15:08:17ZLong non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model10.1038/s41598-018-25702-32045-2322https://doaj.org/article/8a7252ef18474be9b2a1bd0fa497bad52018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25702-3https://doaj.org/toc/2045-2322Abstract Long non-coding RNAs (lncRNAs) have critical regulatory roles in protein-coding gene expression. Aberrant expression profiles of lncRNAs have been observed in various human diseases. In this study, we investigated transcriptome profiles in lung tissues of chronic cigarette smoke (CS)-induced COPD mouse model. We found that 109 lncRNAs and 260 mRNAs were significantly differential expressed in lungs of chronic CS-induced COPD mouse model compared with control animals. GO and KEGG analyses indicated that differentially expressed lncRNAs associated protein-coding genes were mainly involved in protein processing of endoplasmic reticulum pathway, and taurine and hypotaurine metabolism pathway. The combination of high throughput data analysis and the results of qRT-PCR validation in lungs of chronic CS-induced COPD mouse model, 16HBE cells with CSE treatment and PBMC from patients with COPD revealed that NR_102714 and its associated protein-coding gene UCHL1 might be involved in the development of COPD both in mouse and human. In conclusion, our study demonstrated that aberrant expression profiles of lncRNAs and mRNAs existed in lungs of chronic CS-induced COPD mouse model. From animal models perspective, these results might provide further clues to investigate biological functions of lncRNAs and their potential target protein-coding genes in the pathogenesis of COPD.Haiyun ZhangDejun SunDefu LiZeguang ZhengJingyi XuXue LiangChenting ZhangSheng WangJian WangWenju LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haiyun Zhang Dejun Sun Defu Li Zeguang Zheng Jingyi Xu Xue Liang Chenting Zhang Sheng Wang Jian Wang Wenju Lu Long non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model
description	Abstract Long non-coding RNAs (lncRNAs) have critical regulatory roles in protein-coding gene expression. Aberrant expression profiles of lncRNAs have been observed in various human diseases. In this study, we investigated transcriptome profiles in lung tissues of chronic cigarette smoke (CS)-induced COPD mouse model. We found that 109 lncRNAs and 260 mRNAs were significantly differential expressed in lungs of chronic CS-induced COPD mouse model compared with control animals. GO and KEGG analyses indicated that differentially expressed lncRNAs associated protein-coding genes were mainly involved in protein processing of endoplasmic reticulum pathway, and taurine and hypotaurine metabolism pathway. The combination of high throughput data analysis and the results of qRT-PCR validation in lungs of chronic CS-induced COPD mouse model, 16HBE cells with CSE treatment and PBMC from patients with COPD revealed that NR_102714 and its associated protein-coding gene UCHL1 might be involved in the development of COPD both in mouse and human. In conclusion, our study demonstrated that aberrant expression profiles of lncRNAs and mRNAs existed in lungs of chronic CS-induced COPD mouse model. From animal models perspective, these results might provide further clues to investigate biological functions of lncRNAs and their potential target protein-coding genes in the pathogenesis of COPD.
format	article
author	Haiyun Zhang Dejun Sun Defu Li Zeguang Zheng Jingyi Xu Xue Liang Chenting Zhang Sheng Wang Jian Wang Wenju Lu
author_facet	Haiyun Zhang Dejun Sun Defu Li Zeguang Zheng Jingyi Xu Xue Liang Chenting Zhang Sheng Wang Jian Wang Wenju Lu
author_sort	Haiyun Zhang
title	Long non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model
title_short	Long non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model
title_full	Long non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model
title_fullStr	Long non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model
title_full_unstemmed	Long non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model
title_sort	long non-coding rna expression patterns in lung tissues of chronic cigarette smoke induced copd mouse model
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8a7252ef18474be9b2a1bd0fa497bad5
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Long non-coding RNA expression patterns in lung tissues of chronic cigarette smoke induced COPD mouse model

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